Micro gel column technique is fit for detecting mixed fields post ABO incompatible hematopoietic stem cell transplantation

Analysis of ABO chimera from peripheral red cells and reticulocytes by flow cytometry and micro gel column technique in patients post‐ABO‐incompatible HSCT

“…ABO chimera is a natural phenomenon in ABOi HSCT, 10 which accompany with complex and dynamic alteration including A/B antigen and the corresponding IgG or IgM anti-A/B. 6,10 In current, how to choose appropriate methods for diagnosing ABO chimera is very important to not only assess the true status of erythroid engraftment but also achieve personalized safety transfusion. By analysis of different concentration of artificial mixed ABO chimera, we found 5% and 2% positive reactive type A RBC could be accurately detected by MGCT and FCM, respectively.…”

“…ABO chimera is a natural phenomenon in ABOi HSCT, which accompany with complex and dynamic alteration including A/B antigen and the corresponding IgG or IgM anti‐A/B . In current, how to choose appropriate methods for diagnosing ABO chimera is very important to not only assess the true status of erythroid engraftment but also achieve personalized safety transfusion.…”

“…Type A RBCs in A/O mixtures were detected at 10% by both tube method and MGCT, whereas MGCT displayed an indisputable mixed field when it was present. 5,6 Even though flow cytometry (FCM) allows the detection of less than 0.1% of positive cells in artificial mixtures, 7 it is not yet recommended to act as a reference method or available for routine detection. In fact, phenotypic reference method is still highly variable across some institutions.…”

“…Till now, the most popular phenotypic methods for ABO blood typing are classic tube method and microgel column technique (MGCT). Type A RBCs in A/O mixtures were detected at 10% by both tube method and MGCT, whereas MGCT displayed an indisputable mixed field when it was present . Even though flow cytometry (FCM) allows the detection of less than 0.1% of positive cells in artificial mixtures, it is not yet recommended to act as a reference method or available for routine detection.…”