Micro-Incubator Protocol for Testing a CO2 Sensor for Early Warning of Spontaneous Combustion

Abstract

A protocol for detecting the potential occurrence of spontaneous combustion (SC) in stored cottonseeds and peanuts using a micro-incubator is described. The protocol indicates how to quantify CO2 production rates and final CO2 levels in wet versus dry cottonseed and peanut samples, which can provide crucial data for the early detection of SC risk in storage facilities. The experimental design utilizes a micro-incubator to simulate conditions found in large bulk crop storage. Parameters monitored include CO2 concentration, temperature, and relative humidity. The protocol includes preparation methods, experimental procedures for both control (dry) and wet seed tests, and test termination criteria that allow for safe experimentation of likely pathogenic fungi. The protocol has three replicates for wet and dry conditions. The protocol is intended to facilitate future experimental studies and ultimately contribute to the development of a consistently reliable early warning fire detection system for SC in cottonseed and peanut warehouse facilities. A consistently reliable fire detection system would address a critical need in the cotton and peanut industry for improved fire risk management and insurability of storage facilities.