Micro-lightguide spectrophotometry assessment of hepatic and intestinal microcirculation in endotoxemic rats during intravenous treatment with angiotensin II

Schmidt, Götz; Pitz, Laurenz; Markmann, Melanie; Schneck, Emmanuel; Sander, Michael; Koch, Christian; Edinger, Fabian

doi:10.1016/j.ejps.2023.106588

Cited by 2 publications

(5 citation statements)

References 29 publications

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“…Animals treated with V-V ECMO showed increased heart rates. The same results were seen by comparing sham and LPS animals without ECMO in our previous studies [ 13 ]. However, another study by Fujii et al, focusing on V-V ECMO in rats without septic shock, reported no differences between sham and ECMO animals regarding the heart rate [ 18 ].…”

Section: Discussionsupporting

confidence: 87%

“…Since several patients develop abdominal complications during V-V ECMO therapy and the measurement of the intestinal microcirculation is complicated in critically ill patients, the topic of this study is of great interest [ 12 ]. Our working group established the measurement of the hepatic and intestinal microcirculation by micro-lightguide spectrophotometry in a rat model of septic shock without V-V ECMO therapy [ 13 ]. Compared to healthy sham animals, reduced intestinal SO 2 and blood flow were measured during septic shock.…”

Section: Discussionmentioning

confidence: 99%

“…Compared to healthy sham animals, reduced intestinal SO 2 and blood flow were measured during septic shock. Furthermore, while hepatic SO 2 and blood flow did not differ, a reduced relative hemoglobin level was observed during septic shock [ 13 ]. In contrast to this study, the animals were monitored for 3 h, and the microcirculatory measurements were analyzed as a percentage of the baseline.…”

Section: Discussionmentioning

confidence: 99%

“…It cannot be neglected that the probe in this study was placed on the outside of the intestine rather than on the interesting mucosa inside the intestine. However, it must be underlined that the probes of the micro-lightguide spectrophotometry device are designed to measure oxygenation at a depth of between 2 and 4 mm [ 13 ]. Therefore, the results can be interpreted as a surrogate for intestinal microcirculation.…”

Section: Discussionmentioning

confidence: 99%

“…The amount of light absorption induced by hemoglobin was analyzed and expressed in relative hemoglobin arbitrary units (RUs). The second light source emitted laser light (820 nm, 30 mW) determining erythrocyte velocity and consecutive relative blood flow [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

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New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the Rat

Edinger,

Zajonz,

Holtz

et al. 2024

IJMS

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Treatment of critically ill patients with venovenous (V-V) extracorporeal membrane oxygenation (ECMO) has gained wide acceptance in the last few decades. However, the use of V-V ECMO in septic shock remains controversial. The effect of ECMO-induced inflammation on the microcirculation of the intestine, liver, and critically damaged lungs is unknown. Therefore, the aim of this study was to measure the hepatic and intestinal microcirculation and pulmonary inflammatory response in a model of V-V ECMO and septic shock in the rat. Twenty male Lewis rats were randomly assigned to receive V-V ECMO therapy or a sham procedure. Hemodynamic data were measured by a pressure-volume catheter in the left ventricle and a catheter in the lateral tail artery. Septic shock was induced by the intravenous infusion of lipopolysaccharide (1 mg/kg). During V-V ECMO therapy, rats received lung-protective ventilation. The hepatic and intestinal microcirculation was assessed by micro-lightguide spectrophotometry after median laparotomy for 2 h. Systemic and pulmonary inflammation was measured by enzyme-linked immunosorbent assays of plasma and bronchoalveolar lavage (BAL), respectively, which included tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), IL-10, C-X-C motif ligand 2 (CXCL2), and CXCL5. Reduced oxygen saturation and relative hemoglobin concentration were measured in the hepatic and intestinal microcirculation during treatment with V-V ECMO. These animals also showed increased systolic, mean, and diastolic blood pressures. While no differences in left ventricular ejection fraction were observed, animals in the V-V ECMO group presented an increased heart rate, stroke volume, and cardiac output. Blood gas analysis showed dilutional anemia during V-V ECMO, whereas plasma analysis revealed a decreased concentration of IL-10 during V-V ECMO therapy, and BAL measurements showed increased concentrations of TNF-α, CXCL2, and CXCL5. Rats treated with V-V ECMO showed impaired microcirculation of the intestine and liver during septic shock despite increased blood pressure and cardiac output. Despite lung-protective ventilation, increased pulmonary inflammation was recognized during V-V ECMO therapy in septic shock.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the Rat

Edinger,

Zajonz,

Holtz

et al. 2024

IJMS

Self Cite

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New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Veno-Arterial Extracorporeal Membrane Oxygenation in the Rat

Edinger,

Holtz,

Schmidt

et al. 2024

IJMS

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Despite significant efforts toward improving therapy for septic shock, mortality remains high. Applying veno-arterial (V-A) extracorporeal membrane oxygenation (ECMO) in this context remains controversial. Since the cannulation of the femoral artery for V-A ECMO return leads to lower body hyperoxia, this study investigated the impact of V-A ECMO therapy on the intestinal and hepatic microcirculation during septic shock in a rodent model. Thirty male Lewis rats were randomly assigned to receive V-A ECMO therapy with low (60 mL/kg/min) or high (90 mL/kg/min) blood flow or a sham procedure. Hemodynamic data were collected through a pressure-volume catheter in the left ventricle and a catheter in the lateral tail artery. Septic shock was induced by intravenous administration of lipopolysaccharide (1 mg/kg). The rats received lung-protective ventilation during V-A ECMO therapy. The hepatic and intestinal microcirculation was measured by micro-lightguide spectrophotometry after median laparotomy for two hours. Systemic and pulmonary inflammation was detected via enzyme-linked immunosorbent assays (ELISA) of the plasma and bronchoalveolar lavage (BAL), respectively, measuring tumor necrosis factor-alpha (TNF-α), interleukins 6 (IL-6) and 10 (IL-10), and C-X-C motif ligands 2 (CXCL2) and 5 (CXCL5). Oxygen saturation and relative hemoglobin concentration were reduced in the hepatic and intestinal microcirculation during V-A ECMO therapy, independent of the blood flow rate. Further, rats treated with V-A ECMO therapy also presented elevated systolic, diastolic, and mean arterial blood pressure and increased stroke volume, cardiac output, and left ventricular end-diastolic volume. However, left ventricular end-diastolic pressure was only elevated during high-flow V-A ECMO therapy. Blood gas analysis revealed a dilutional anemia during V-A ECMO therapy. ELISA analysis showed an elevated plasma CXCL2 concentration only during high-flow V-A ECMO therapy and elevated BAL CXCL2 and CXCL5 concentrations only during low-flow V-A ECMO therapy. Rats undergoing V-A ECMO therapy exhibited impaired microcirculation of the intestine and liver during septic shock despite increased blood pressure and cardiac output. Increased pulmonary inflammation was detected only during low-flow V-A ECMO therapy in septic shock.

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Micro-lightguide spectrophotometry assessment of hepatic and intestinal microcirculation in endotoxemic rats during intravenous treatment with angiotensin II

Cited by 2 publications

References 29 publications

New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the Rat

New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the Rat

New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Veno-Arterial Extracorporeal Membrane Oxygenation in the Rat

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