Micro-mechanical properties of corneal scaffolds from two different bio-models obtained by an efficient chemical decellularization

Marin-Tapia, Hugo Arian; Romero-Salazar, L.; Arteaga‐Arcos, Juan Carlos; Rosales-Ibáñez, Raúl; Mayorga-Rojas, M.

doi:10.1016/j.jmbbm.2021.104510

Cited by 12 publications

(15 citation statements)

References 48 publications

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“…Surfactants used for corneal decellularization have mainly been ionic (such as sodium dodecyl sulfate, SDS) and (Triton X-100) 40 . Similarly, zwitterionic surfactants, like the most widely used agent of this type, 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS) has also been effective in isolating the corneal ECM from cellular components 41 . Other reports have shown that neutral hydrophilic groups of zwitterionic detergents can protect native proteins during decellularization 1 .…”

Section: Discussionmentioning

confidence: 99%

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Pantić

Cumic

Valjarević

et al. 2023

Preprint

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Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking native tissue and reducing the risk of immune rejection post-transplantation. Despite great success in achieving acellular scaffolds, little consensus exists regarding the quality of the decellularized extracellular matrix (ECM). Metrics used to evaluate ECM performance are study-specific, subjective, and semi-quantitative. Thus, this work focused on developing a computational method to examine the effectiveness of corneal decellularization. We combined conventional semi-quantitative histological assessments and automated scaffold evaluations based on textual image analyses to assess decellularization efficiency. Our study highlights that it is possible to develop contemporary artificial intelligence (AI) models based on random forests and support vector machine algorithms, which can identify regions of interest in acellularized corneal stromal tissue with relatively high accuracy. These results provide a platform for developing AI biosensing systems for evaluating fine morphological changes in decellularized scaffolds crucial for evaluating their functionality.

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Section: Discussionmentioning

confidence: 99%

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Pantić

Cumic

Valjarević

et al. 2023

Preprint

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“…However, the decellularization process may significantly alter the structure of the tissue ECM and, thus, the biomechanical properties of the decellularized scaffolds, which could alter the phenotype, proliferation capability, and differentiation potential of the cells cultured on this scaffold and modify cell behavior and tissue regeneration [51]. In fact, it has been demonstrated that decellularized corneal xenografts vary their biomechanical properties after decellularization [52]. Therefore, a thorough analysis of the biomechanical properties of the DL generated in the present work is in need.…”

Section: Discussionmentioning

confidence: 99%

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

et al. 2021

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Patients with severe limbal damage and limbal stem cell deficiency are a therapeutic challenge. We evaluated four decellularization protocols applied to the full-thickness and half-thickness porcine limbus, and we used two cell types to recellularize the decellularized limbi. The results demonstrated that all protocols achieved efficient decellularization. However, the method that best preserved the transparency and composition of the limbus extracellular matrix was the use of 0.1% SDS applied to the half-thickness limbus. Recellularization with the limbal epithelial cell line SIRC and human adipose-derived mesenchymal stem cells (hADSCs) was able to generate a stratified epithelium able to express the limbal markers p63, pancytokeratin, and crystallin Z from day 7 in the case of SIRC and after 14–21 days of induction when hADSCs were used. Laminin and collagen IV expression was detected at the basal lamina of both cell types at days 14 and 21 of follow-up. Compared with control native limbi, tissues recellularized with SIRC showed adequate picrosirius red and alcian blue staining intensity, whereas limbi containing hADSCs showed normal collagen staining intensity. These preliminary results suggested that the limbal substitutes generated in this work share important similarities with the native limbus and could be potentially useful in the future.

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“… 68 Its reduced permeating qualities limit CHAPS’ ability to remove nuclear DNA, thereby making CHAPS more applicable for decellularization of thin tissues. 68 – 70 CHAPS also highly retains the biomechanical properties of native tissue, making it ideal for tissues, which must be capable of contracting with ease such as heart or lung. 70 Other zwitterionic detergents Sulfobetaine 10 and Sulfobetaine 16 (SB10 and SB16, respectively) induce cell apoptosis, resulting in improved cell removal, which eliminates the need for vigorous washing steps.…”

Section: Decellularization Techniquesmentioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Micro-mechanical properties of corneal scaffolds from two different bio-models obtained by an efficient chemical decellularization

Cited by 12 publications

References 48 publications

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

Decellularization for the retention of tissue niches

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