Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing

Litowczenko, Jagoda; Wychowaniec, Jacek K.; Załęski, Karol; Marczak, Łukasz; Edwards-Gayle, Charlotte J.C.; Tadyszak, Krzysztof; Maciejewska, Barbara M.

doi:10.1016/j.bioadv.2023.213653

Cited by 9 publications

(1 citation statement)

References 58 publications

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“…Indeed, once the effect of 2D materials on the players involved in nerve regeneration is unravelled, it would be possible to integrate the materials onto a biopolymer support to realize a planar polymeric structure rolled to form tubular nerve conduits. In the literature there are many examples of 3D tubes prepared from a planar scaffold that can be simply rolled along the lengthwise edge and taped, 122 implanted and wrapped directly around the nerve stumps 123 or prepared using self-rolling films. 124 Similar examples are also between FDA-approved nerve grafts, such as NeuraWrap™ (Integra LifeSciences Co.) that is used by wrapping around the injured nerves, or NeuroMend™ (Collagen Matric, Inc.) that can be unrolled and self-curled to match the dimensions of the injured tissue.…”

Section: Discussionmentioning

confidence: 99%

Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Convertino,

Nencioni,

Russo

et al. 2024

Nanoscale

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Section: Discussionmentioning

confidence: 99%

Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Convertino,

Nencioni,

Russo

et al. 2024

Nanoscale

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Application of Adipose Extracellular Matrix and Reduced Graphene Oxide Nanocomposites for Spinal Cord Injury Repair

Verstappen,

Bieler,

Barroca

et al. 2024

Adv Healthcare Materials

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Graphene‐based materials (GBMs) hold strong promise to restore the spinal cord microenvironment and promote functional recovery after spinal cord injury (SCI). Nanocomposites consisting of reduced graphene oxide (rGO) and adipose tissue‐derived extracellular matrix (adECM) are known to promote neuronal growth in vitro and to evoke a biocompatible response in vivo when implanted on top of the intact spinal cord. In this study, pristine adECM and adECM‐rGO nanocomposites are implanted directly after hemisection SCI in rats. Scaffolds composed of collagen type I (COL) are applied as negative control, based on evidence that COL triggers integrin‐mediated astrogliosis. However, COL scaffolds induce orthotopic bone formation in the lesion site and are therefore excluded from further analyses. Compared to pristine adECM, adECM‐rGO nanocomposites completely restore spinal cord integrity. Macrophage‐mediated uptake and clearance of rGO remnants is observed as early as 3 weeks post‐implantation. Nanocomposites show an elevated presence of βIII‐tubulin‐positive axons in the host‐material interface after 8 weeks, yet scaffold penetration by axons is only occasionally observed. This is partially due to an increased expression of chondroitin sulfate proteoglycans (CSPGs) within the nanocomposites, even though reactive astrogliosis is unaltered. Despite the complete restoration of tissue architecture, adECM‐rGO treatment does not significantly improve functional recovery.

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Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

Ozcicek,

Aysit,

Balcikanli

et al. 2024

Macromolecular Bioscience

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Spinal cord injuries are very common worldwide, leading to permanent nerve function loss with devastating effects in the affected patients. The challenges and inadequate results in the current clinical treatments are leading scientists to innovative neural regenerative research. Advances in nanoscience and neural tissue engineering have opened new avenues for SCI treatment. In order for designed nerve guidance conduit to be functionally useful, it must have ideal scaffold properties and topographic features that promote the linear orientation of damaged axons. In this study, we aimed to develop channeled polycaprolactone (PCL)/Poly‐D,L‐lactic‐co‐glycolic acid (PLGA) hybrid film scaffolds, modify their surfaces by IKVAV pentapeptide/gold nanoparticles (AuNPs) or polypyrrole (PPy) and investigate the behavior of motor neurons on the designed scaffold surfaces in vitro under static/bioreactor conditions. We also examined their potential to promote neural regeneration after implantation into the rat spinal cord injury by shaping the film scaffolds modified with neural factors into a tubular form. We show that channeled groups decorated with AuNPs highly promote neurite orientation under bioreactor conditions and also the developed optimal nerve guidance conduit (PCL/PLGA G1‐IKVAV/BDNF/NGF‐AuNP50) highly regenerates spinal cord injury. The results indicated that the designed scaffold could be an ideal candidate for spinal cord regeneration.This article is protected by copyright. All rights reserved

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Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing

Cited by 9 publications

References 58 publications

Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Application of Adipose Extracellular Matrix and Reduced Graphene Oxide Nanocomposites for Spinal Cord Injury Repair

Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

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Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing

Cited by 9 publications

References 58 publications

Interaction of graphene and WS2with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Interaction of graphene and WS2with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Application of Adipose Extracellular Matrix and Reduced Graphene Oxide Nanocomposites for Spinal Cord Injury Repair

Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

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Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration

Interaction of graphene and WS₂with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration