Micro‐pillar array columns (µPAC): An efficient tool for comparing tissue and cultured cells of glioblastoma

Berg, Henriette Engen; Halldórsson, Skarphéðinn; Bakketeig, Eris Aas; Thiede, Bernd; Sandberg, Cecilie; Lundanes, Elsa; Vik-Mo, Einar O.; Wilson, Steven Ray

doi:10.1016/j.jcoa.2022.100047

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…μPACs have, given their intrinsic suitability for miniaturization, found widespread use in the area of proteomics in the years that passed since their initial development. − Next to this, μPACs have also already been frequently used in metabolomics to benefit from the large plate numbers offered by the longest μPACs. , …”

Section: Introductionmentioning

confidence: 99%

On-Chip Comparison of the Performance of First- and Second-Generation Micropillar Array Columns

Vankeerberghen,

Op de Beeck,

Desmet

2023

Anal. Chem.

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Because of its dimensions, the recently introduced micropillar array columns are most suited for high-efficiency liquid chromatography separations in proteomics. Unlike the packed bed columns and capillary-based column formats, the micropillar array concept still has significant room to progress in terms of the reduction of its characteristic size (i.e., pillar diameter and interpillar distance) to open the road to even higher-efficiency separations and their applications. We report here on the on-chip comparison between first-generation (Gen 1) and second-generation (Gen 2) micropillar array columns wherein the pillar and interpillar size have been halved. Because of the on-chip measurements, the observed plate heights H represent the fundamental band broadening, devoid of any extra-column band-broadening effects. The observed reduction of H with a factor of 2 around the u opt-velocity and with a factor of 4 in the C-term dominated regime of the van Deemter-curve is in full agreement with the theoretically expected gain. This shows the pillar and interpillar size reduction could be effectuated without affecting the theoretical separation potential of the micropillar arrays. Compared to Gen 1, Gen 2 offers a 4-fold reduction of the required analysis time around the optimal velocity and about a 16-fold reduction in the C-term-dominated range.

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Section: Introductionmentioning

confidence: 99%

On-Chip Comparison of the Performance of First- and Second-Generation Micropillar Array Columns

Vankeerberghen,

Op de Beeck,

Desmet

2023

Anal. Chem.

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“…The same holds for the multi-capillary column with diffusive bridging concept recently introduced by Parmentier [18]. One other approach, based on using photolithography and plasma etching to produce silicon-based micro-pillar array columns has made it to commercialization in the area of nano-LC for proteomics [8,[19][20][21][22][23]. Although the induced order and the enlarged flow resistance of the micro-pillar array columns indeed lead to groundbreaking performances, these columns are silicon-based and currently offer a very limited choice of stationary phases.…”

Section: Introductionmentioning

confidence: 93%

Structured microgroove columns as a potential solution to obtain perfectly ordered particle beds

Vankeerberghen

Verloy

Jimidar

et al. 2023

Journal of Chromatography A

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“…μPACs are fabricated from silicon wafers using standard lithographic patterning and etching . Because of the difficulties to maintain a uniform interpillar distance when etching too deep (so-called aspect ratio problem), μPACs have a limited depth and are therefore in their current format most suited for nano- and microflow liquid chromatography. − In this area, μPACs are now getting more and more widespread used in a broad variety of proteomics and metabolomics subdisciplines. − Their miniaturized format also makes them inherently suited for single cell studies. , …”

Section: Introductionmentioning

confidence: 99%

Column-Only Band Broadening in a Porous Shell Radially Elongated Pillar Array Column

Vankeerberghen,

Op de Beeck,

Desmet

2024

Anal. Chem.

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In the quest for better performing separation media for liquid chromatography, micropillar array columns have received great interest over the past years. While previous research was mainly focused around micropillar array columns (μPACs) filled with cylindrical pillars, this contribution discusses μPACs with rectangular pillars, which, for the first time, have been anodized and hence carry a mesoporous shell. We report on a series of on-chip measurements of the band broadening and flow permeability in a μPAC with very wide radially elongated pillars (3•75 μm) and with an interpillar distance (2 μm) between that of the first (2.5 μm) and second generation (1.25 μm) of cylindrical μPACs. Because of the extreme flow path tortuosity, this type of μPAC can produce very large plate numbers over a short distance. Despite the relatively large interpillar distance, we obtain H min = 0.26 μm for a nearly unretained component (phase retention factor, k′ ≈ 0.24) and H min = 0.79 μm for a retained component with k′ ≈ 3. The kinetic performance in terms of separation impedance (E i = 19) is considerably improved compared to cylindrical pillar μPACs (E i in range 40−50) and is in excellent agreement with the theoretical value for an open tubular channel with a rectangular cross-section (E i = 18). This shows that rectangular μPACs can be represented as a parallel bundle of interconnected open-tubular channels.

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Micro‐pillar array columns (µPAC): An efficient tool for comparing tissue and cultured cells of glioblastoma

Cited by 9 publications

References 33 publications

On-Chip Comparison of the Performance of First- and Second-Generation Micropillar Array Columns

On-Chip Comparison of the Performance of First- and Second-Generation Micropillar Array Columns

Structured microgroove columns as a potential solution to obtain perfectly ordered particle beds

Column-Only Band Broadening in a Porous Shell Radially Elongated Pillar Array Column

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