Micro Propagation and Plant Strengthening of Tissue Cultured Plants, Inoculated with Several Bacterial Strains

Panigrahi, Sunitha; Lakshmi, K. S.; Mir, Nida

doi:10.15373/22778179/aug2013/7

Cited by 3 publications

(2 citation statements)

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“…The macro and micro nutrient analysis was determined by Perkin-Elmer Analyst, 300 single beam Atomic Absorption Spectrophotometer and the data was obtained and documented [10] .…”

Section: Nutrient Analysismentioning

confidence: 99%

Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content

Panigrahi

Lakshmi

Venkateshwarulu

et al. 2014

SpringerBriefs in Applied Sciences and Technology

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The ability to grow plant tissues in vitro and to control their development forms the basis of many practical applications in agriculture, horticulture and industrial chemistry. Establishment of a cell, tissue or organ culture and regeneration of plantlets under in vitro conditions has opened up new avenues in the area of plant biotechnology. Micropropagation is one such method where the plants are grown under in vitro conditions to provide a better survival rate and mass propagation in a short time, it is achieved through the establishment of the explants and their initial growth in vitro, followed by transferring them onto the field or in a greenhouse. This has triggered a great interest in understanding plant physiology and performing a comprehensive study regarding the change in physiology and morphology under influence of various useful bacterial combinations. In this review, plant growth promoting rizobacteria (PGPR) were used in the bio priming of the micro propagated plants (banana), these plants showed increased growth of the root length; shoot length, internode diameter and number of leaves. All parts of the banana plant have medicinal applications: the flowers in bronchitis and dysentery and on ulcers; cooked flowers are given to diabetics; the astringent plant sap in cases of hysteria, epilepsy, leprosy, fevers, hemorrhages, acute dysentery and diarrhea, and it is applied on hemorrhoids, insect and other stings and bites; young leaves are placed as poultices on burns and other skin afflictions;

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“…The macro and micro nutrient analysis was determined by Perkin-Elmer Analyst, 300 single beam Atomic Absorption Spectrophotometer and the data was obtained and documented [10] .…”

Section: Nutrient Analysismentioning

confidence: 99%

Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content

Panigrahi

Lakshmi

Venkateshwarulu

et al. 2014

SpringerBriefs in Applied Sciences and Technology

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“…Currently, there are numerous methods utilized for detection of the genetic fidelity of micropropagated plants [13]. Molecular markers like SSR and ISSR are powerful techniques for identification of genetic variation [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Genetic Identity and Fidelity of Banana Genotype Musa acuminata through Molecular Fingerprinting

More¹,

Janvale²,

Wagh

et al. 2023

AJAHR

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Micropropagation for extensive production of elite planting materials is an effective and superior alternative to suckers and conventional means of propagation of Musa spp. The present investigation was carried out to evaluate the genetic identity of different micropropagated banana genotypes Grand Naine, Shrimantee and Basrai. Further micropropagated banana genotypes Grand Naine comparing with mother plant during different stages of subculture using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR). Moreover, for micropropagation explants (suckers) were inoculated on initiation medium containing MS (Murashige and Skoog) supplemented with 5 mg/l BAP (Benzyl amino purine) and 39 mg/l AdSO4 (Adenine sulphate). The second, third and fourth subcultures were carried out to separate all multiple shoots. Furthermore, for rooting MS media fortified with growth hormones viz., 2 mg/l BAP, 1mg/l IBA (Indole-3-butyric Acid) and 200mg/l activated charcoal were used under present investigation. Findings revealed that 90 % and 75 % plantlets were successfully established during primary hardening in controlled and natural field condition. Further, 26 primers were screened from that 20 primers (ISSR and SSR) showed 146 clear, reproducible and scorable bands ranging from 100-2000 base pairs produced. The banding pattern of these randomly selected plants was monomorphic with mother plant of all selected genotype that confirmed the clonal uniformity.

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Biopriming of Micropropagated Plantlets Using Various Bacterial Strains in Different Combinations-Induces the Systemic Resistance

Panigrahi¹,

Lakshmi

Mir³

et al. 2015

Next Generation DNA Led Technologies

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Micro Propagation and Plant Strengthening of Tissue Cultured Plants, Inoculated with Several Bacterial Strains

Cited by 3 publications

References 0 publications

Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content

Biohardening of Micropropagated Plants with PGPR and Endophytic Bacteria Enhances the Protein Content

Deciphering the Genetic Identity and Fidelity of Banana Genotype Musa acuminata through Molecular Fingerprinting

Biopriming of Micropropagated Plantlets Using Various Bacterial Strains in Different Combinations-Induces the Systemic Resistance

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