Micro-Raman spectroscopic study of thyroid tissues

Neto, Lázaro Pinto Medeiros; Carvalho, Luis Felipe das Chagas e Silva de; Santos, Laurita dos; Soto, Claudio A. Telléz; Canevari, Renata de Azevedo; Santos, André Bandiera de Oliveira; Mello, Evandro Sobroza de; Pereira, Marina Aparecida; Cernea, Cláudio Roberto; Brandão, Lenine Garcia; Martin, Aírton Abrahão

doi:10.1016/j.pdpdt.2016.11.018

Cited by 16 publications

(15 citation statements)

References 40 publications

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“…The patients and control subjects in our study were selected from nonsmoker volunteers, with no history of occupational or environmental exposure to known genotoxic chemicals, which may affect CBMN-cyt assay parameters and plasma 8-OHdG levels. Similarly, secondary factors, such as elevated TSH, may be involved in the development of multinodular goiters [30, 31]. However, we found that there was a poor association between apoptotic cells from CBMN-cyt assay parameters and TSH in patients with multinodular goiter.…”

Section: Discussionmentioning

confidence: 60%

“…These findings are potentially significant, as it is currently not known whether the benign nodular thyroid progresses, resulting in malignancy. Furthermore, accumulation of DNA mutations (higher replication rate or failure of mutation repair) may contribute to the pathogenesis of thyroid cancer [9, 11, 30–32]. Therefore, our results may indicate that monitoring of MN frequency and 8-OHdG levels is part of the necessary follow-up of patients with multinodular goiter in order to monitor the increased malignancy risk.…”

Section: Discussionmentioning

confidence: 98%

“…It is known that thyroid hormone synthesis is associated with increased H 2 O 2 production and free radical formation, which may damage genomic DNA and cause mutations [30, 31]. Therefore, oxidative stress and oxidative base adducts could contribute to the development of thyroid cancer [30, 32, 33]. However, the chromosomal and oxidative DNA damage, cytotoxicity, and the cytostasis status of patients with multinodular goiter remain unclear.…”

Section: Discussionmentioning

confidence: 99%

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Increased Chromosomal and Oxidative DNA Damage in Patients with Multinodular Goiter and Their Association with Cancer

Dönmez-Altuntaş

Bayram

Bıtgen

et al. 2017

International Journal of Endocrinology

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Thyroid nodules are a common clinical problem worldwide. Although thyroid cancer accounts for a small percentage of thyroid nodules, the majority are benign. 8-Hydroxy-2′-deoxyguanosine (8-OHdG) levels are a marker of oxidative stress and play a key role in the initiation and development of a range of diseases and cancer types. This study evaluates cytokinesis-block micronucleus cytome (CBMN-cyt) assay parameters and plasma 8-OHdG levels and their association with thyroid nodule size and thyroid hormones in patients with multinodular goiter. The study included 32 patients with multinodular goiter and 18 age- and sex-matched healthy controls. CBMN-cyt assay parameters in peripheral blood lymphocytes of patients with multinodular goiter and controls were evaluated, and plasma 8-OHdG levels were measured. The micronucleus (MN) frequency (chromosomal DNA damage), apoptotic and necrotic cells (cytotoxicity), and plasma 8-OHdG levels (oxidative DNA damage) were significantly higher among patients with multinodular goiter. Our study is the first report of increased chromosomal and oxidative DNA damage in patients with multinodular goiter, which may predict an increased risk of thyroid cancer in these patients. MN frequency and plasma 8-OHdG levels may be markers of the carcinogenic potential of multinodular goiters and could be used for early detection of different cancer types, including thyroid cancer.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Increased Chromosomal and Oxidative DNA Damage in Patients with Multinodular Goiter and Their Association with Cancer

Dönmez-Altuntaş

Bayram

Bıtgen

et al. 2017

International Journal of Endocrinology

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“…In a previous study by Medeiros‐Neto et al, in which normal and pathological tissues of the thyroid gland were evaluated using confocal Raman spectroscopy, 78.3% discrimination was observed in the comparison between normal and cancerous tissues by the LDA technique with cross‐validation. Sixty‐five percent was obtained in comparison between normal and goiter tissue, whereas 75% discrimination was found between goiter and cancer.…”

Section: Resultsmentioning

confidence: 92%

“…Sixty‐five percent was obtained in comparison between normal and goiter tissue, whereas 75% discrimination was found between goiter and cancer. In order to verify these values, the binary logistic regression test was performed on the same data, providing a concordance value, that is, a discrimination of 89.2% for the comparison between normal and cancer, of 80.6% between normal and goiter and of 85.7% for the comparison goiter and cancer tissues . Subsequently, we evaluated the same samples using the IR technique in order to compare and complement the results.…”

Section: Resultsmentioning

confidence: 99%

Infrared and confocal Raman spectroscopy to differentiate changes in the protein secondary structure in normal and abnormal thyroid tissues

Soto

Medeiros-Neto

Santos

et al. 2018

J Raman Spectroscopy

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Thirty thyroid samples of normal and abnormal tissues were analyzed by infrared and confocal Raman Spectroscopy. We studied the Amide I (1,720–1,580 cm−1) spectral region in order to determine different components of the proteins' secondary structure in the samples. Peak positions of the Amide I bands were determined using the second derivative and Fourier self‐deconvolution of infrared and Raman spectra. We obtained band areas corresponding to β‐turn, 310‐helix, α‐helix, β‐sheet, β‐turns, and side chains for the infrared and confocal Raman spectra by Gaussian fitting. Using the second derivative of the infrared and Raman spectra, we demonstrated the consistency of the vibrational assignments because compounds of low symmetry without an inversion center present coincident bands that differ only in intensity; hence, the infrared procedures to identify and assign the bands of the proteins' secondary structure in the Raman spectra are valid. The results obtained are supported by vibrational theory and indicate that infrared and confocal Raman spectroscopy are important, useful, and fast tools to identify changes in the proteins' secondary structure in normal tissues, goiter, and follicular and papillary thyroid carcinomas.

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Raman spectroscopy with an improved support vector machine for discrimination of thyroid and parathyroid tissues

Hu,

Xing,

Shao

et al. 2024

Journal of Biophotonics

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The objective of this study was to discriminate thyroid and parathyroid tissues using Raman spectroscopy combined with an improved support vector machine (SVM) algorithm. In thyroid surgery, there is a risk of inadvertently removing the parathyroid glands. At present, there is a lack of research on using Raman spectroscopy to discriminate parathyroid and thyroid tissues. In this article, samples were obtained from 43 individuals with thyroid and parathyroid tissues for Raman spectroscopy analysis. This study employed partial least squares (PLS) to reduce dimensions of data, and three optimization algorithms are used to improve the classification accuracy of SVM algorithm model in spectral analysis. The results show that PLS‐GA‐SVM algorithm has higher diagnostic accuracy and better reliability. The sensitivity of this algorithm is 94.67% and the accuracy is 94.44%. It can be concluded that Raman spectroscopy combined with the PLS‐GA‐SVM diagnostic algorithm has significant potential for discriminating thyroid and parathyroid tissues.

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Micro-Raman spectroscopic study of thyroid tissues

Cited by 16 publications

References 40 publications

Increased Chromosomal and Oxidative DNA Damage in Patients with Multinodular Goiter and Their Association with Cancer

Increased Chromosomal and Oxidative DNA Damage in Patients with Multinodular Goiter and Their Association with Cancer

Infrared and confocal Raman spectroscopy to differentiate changes in the protein secondary structure in normal and abnormal thyroid tissues

Raman spectroscopy with an improved support vector machine for discrimination of thyroid and parathyroid tissues

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