Micro-RNA–Regulated Proangiogenic Signaling in Arteriovenous Loops in Patients with Combined Vascular and Soft-Tissue Reconstructions: Revisiting the Nutrient Flap Concept

Henn, Dominic; Abu‐Halima, Masood; Falkner, Frank; Wermke, Dominik; Meme, Lilian G; Kühner, Clemens; Keller, Andreas; Kneser, Ulrich; Meese, Eckart

doi:10.1097/prs.0000000000004750

Cited by 11 publications

(16 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative abundance level for each miRNA was calculated using the 2 −ΔΔCT equation [23]. RNU6B small nuclear RNA (snRNA) was used an endogenous reference control for normalization purpose as previously described [12,13,22,[24][25][26][27] and because of its minimum abundance variance between the TS patients and HVs as observed by DataAssist™Software (Applied Biosystems). Correlations were computed using Spearman's regression coefficient and the difference between the groups was analyzed using a Mann-Whitney-U test using GraphPad Prism 7.0.…”

Section: Discussionmentioning

confidence: 99%

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Turner syndrome (TS) is a chromosomal disorder, in which a female is partially or entirely missing one of the two X chromosomes, with a prevalence of 1:2500 live female births. The present study aims to identify a circulating microRNA (miRNA) signature for TS patients with and without congenital heart disease (CHD). Methods Microarray platform interrogating 2549 miRNAs were used to detect the miRNA abundance levels in the blood of 33 TS patients and 14 age-matched healthy volunteer controls (HVs). The differentially abundant miRNAs between the two groups were further validated by RT-qPCR. Results We identified 60 differentially abundant miRNA in the blood of TS patients compared to HVs, from which, 41 and 19 miRNAs showed a higher and a lower abundance levels in TS patients compared to HVs, respectively. RT-qPCR confirmed the significantly higher abundance levels of eight miRNAs namely miR-374b-5p, miR-199a-5p, miR-340-3p, miR-125b-5p, miR-30e-3p, miR-126-3p, miR-5695, and miR-26b-5p in TS patients as compared with the HVs. The abundance level of miR-5695 was higher in TS patients displaying CHD as compared to TS patients without CHD (p = 0.0265; log2-fold change 1.99); whereas, the abundance level of miR-126-3p was lower in TS patients with congenital aortic valve disease (AVD) compared to TS patients without BAV (p = 0.0139, log2-fold change 1.52). The clinical feature statistics revealed that miR-126-3p had a significant correlation with sinotubular junction Z-score (r = 0.42; p = 0.0154).

show abstract

Section: Discussionmentioning

confidence: 99%

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…MicroRNAs (miRNAs/miRs) are non-coding single- stranded small molecule RNAs that exert their biological function by inhibiting the transcription and translation of target genes, via binding to the 3′ non-coding region of the target mRNA (8,9). Previous studies have demonstrated that miRNAs are closely associated with the occurrence and development of numerous tumors, including ovarian cancer, liver cancer, stomach cancer and breast cancer (10–13).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of miR‑26b‑5p on thyroid cancer

et al. 2019

View full text Add to dashboard Cite

In order to examine the inhibitory effects of microRNA (miR)-26b-5p on thyroid cancer (TC), the clinicopathological features and pathological tissues of 67 patients were collected. The expression levels of miR-26b-5p were detected in TC and paracarcinoma tissues by quantitative polymerase chain reaction, and the association between miR-26b-5p expression and the clinicopathological features of the patients was analyzed using t-test or one-way analysis of variance. In addition, B-CPAP TC cells were infected with a lentivirus to induce miR-26b-5p overexpression and proliferation was detected by Cell Counting kit-8. Subsequently, migration and invasion were detected by Transwell and Matrigel assays, respectively, and the molecular mechanism of action was investigated by western blotting. The results demonstrated that the expression levels of miR-26b-5p were significantly lower in TC tissues compared with paracarcinoma tissues (P<0.01), and miR-26b-5p was associated with lymph node metastasis (P<0.05). In addition, overexpression of miR-26b-5p inhibited the proliferation, invasion and migration of B-CPAP cells. Western blot analysis demonstrated that the protein expression levels of phosphorylated glycogen synthase kinase-3β (pGsk-3β) were decreased, and the expression of β-catenin was decreased in B-CPAP cells overexpressing miR-26b-5p. These results demonstrated that miR-26b-5p may exert antitumor activity. In addition, at the molecular level, these effects may be associated with the Gsk-3β/β-catenin pathway.

show abstract

“…< 1.5 in the peripheral blood and the vascular wall of AV loops (POD 10 after loop placement). The complete miRNA expression profiles in vascular AV loop tissue were previously published 10 . ( c,d ) Correlation between miRNA expression profiles in blood and vascular tissue reveals 5 concordantly up-regulated miRNAs ( d , upper right quadrant), 2 concordantly down-regulated miRNAs ( d, lower left quadrant), and 4 discordantly deregulated miRNAs ( d , upper left and lower right quadrants).…”

Section: Resultsmentioning

confidence: 99%

“…The goal of our study was to determine the impact of two-stage AV loop reconstructions on the expression levels of circulating miRNAs and the serum levels of NT-proBNP and copeptin as established cardiac biomarkers, in order to provide guidance for risk stratification, selection of operative technique and timing in microsurgical reconstructions with AV loops. Moreover, we correlated the miRNA signatures from peripheral blood of AV loop patients with previously determined miRNA expression profiles from human venous AV loop tissue 10 to identify systemic perturbations of miRNA expression after AV loop placement across different tissue types.…”

Section: Introductionmentioning

confidence: 99%

A multivariable miRNA signature delineates the systemic hemodynamic impact of arteriovenous shunt placement in a pilot study

Henn

Abu‐Halima

Kahraman

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Arteriovenous (AV) fistulas for hemodialysis can lead to cardiac volume loading and increased serum brain natriuretic peptide (BNP) levels. Whether short-term AV loop placement in patients undergoing microsurgery has an impact on cardiac biomarkers and circulating microRNAs (miRNAs), potentially indicating an increased hemodynamic risk, remains elusive. Fifteen patients underwent AV loop placement with delayed free flap anastomosis for microsurgical reconstructions of lower extremity soft-tissue defects. N-terminal pro-BNP (NT-proBNP), copeptin (CT-proAVP), and miRNA expression profiles were determined in the peripheral blood before and after AV loop placement. MiRNA expression in the blood was correlated with miRNA expression from AV loop vascular tissue. Serum NT-proBNP and copeptin levels exceeded the upper reference limit after AV loop placement, with an especially strong NT-proBNP increase in patients with preexistent cardiac diseases. A miRNA signature of 4 up-regulated (miR-3198, miR-3127-5p, miR-1305, miR-1288-3p) and 2 down-regulated miRNAs (miR30a-5p, miR-145-5p) which are related to cardiovascular physiology, showed a significant systemic deregulation in blood and venous tissue after AV loop placement. AV loop placement causes serum elevations of NT-proBNP, copeptin as well as specific circulating miRNAs, indicating a potentially increased hemodynamic risk for patients with cardiovascular comorbidities, if free flap anastomosis is delayed.

show abstract

Micro-RNA–Regulated Proangiogenic Signaling in Arteriovenous Loops in Patients with Combined Vascular and Soft-Tissue Reconstructions: Revisiting the Nutrient Flap Concept

Cited by 11 publications

References 52 publications

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients

Inhibitory effects of miR‑26b‑5p on thyroid cancer

A multivariable miRNA signature delineates the systemic hemodynamic impact of arteriovenous shunt placement in a pilot study

Contact Info

Product

Resources

About