Micro-Scale Photofluorometric Determination of "Free Erythrocyte Porphyrin" (Protoporphyrin IX)

Chisolm, J. Julian; Brown, Dean H.

doi:10.1093/clinchem/21.11.1669

Cited by 125 publications

(31 citation statements)

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“…ALAD activity was measured <5 hr after blood sampling (blood was stored at 48C) using the European standardized method [Berlin and Schaller, 1974]. EP was measured by spectrofluorometric method [Chisolm and Brown, 1975] and the accuracy controlled by the laboratory's regular participation in the proficiency testing program for protoporphyrin in blood (Centers for Disease Control and Prevention, Atlanta, GA). Hct was measured by standard hematologic method.…”

Section: Blood Sampling and Analysesmentioning

confidence: 99%

Lead effect on blood pressure in moderately lead‐exposed male workers

Telišman

Pizent

Jurasović

et al. 2004

American J Industrial Med

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Section: Blood Sampling and Analysesmentioning

confidence: 99%

Lead effect on blood pressure in moderately lead‐exposed male workers

Telišman

Pizent

Jurasović

et al. 2004

American J Industrial Med

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“…Erythrocytes and plasma from the subjects were assayed for PP IX according to the method of Chisolm and Brown (1975). After incubation and spectrophotometric measurements, the supernatant of the samples was also assayed for PP IX to determine if any leaked from the erythrocytes.…”

Section: Protoporphyrin IX Determinationmentioning

confidence: 99%

Light‐dependent Generation of Superoxide From Human Erythrocytes

Bynoe

Gottsch

Pou

et al. 1992

Photochem & Photobiology

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Using the cytochrome c reduction method, we investigated light-dependent erythrocytic superoxide production. After 4 h light and dark exposure of erythrocytes from eight healthy human subjects, an average of 18.6% more superoxide was generated by erythrocytes exposed to light. Pretreatment of erythrocytes with the superoxide dismutase inhibitor N,N-diethyldithiocarbamate increased detection of superoxide while pretreatment with the anion channel blocker 4-acetamido-4'-isothiocyano-2,2'-disulfonic acid stilbene decreased detection. These findings indicate that substantially more spontaneously generated superoxide is produced and escapes from normal erythrocytes at ambient oxygen tensions on exposure to light. This excess generation and escape of superoxide from erythrocytes exposed to light may result in tissue photosensitization, especially in the retina of the eye, where high oxygen tension, blood and chronic light exposure occur simultaneously.

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“…Blood lead levels were measured against aqueous standards prepared from aliquots of the lead stock solution diluted in 0.5% (v/v) HN03 [Fernandez, 19751. Protoporphyrin Determination FEP assay. FEP levels were determined by the method of Chisolm and Brown [1977]. Triplicate 20 pl aliquots of whole blood were extracted with an ethyl acetate (ACS Spectroanalyzed, Fisher Scientific Co., Springfield, NJ)-glacial acetic acid (reagent grade, Fisher Scientific Co.) solution (3: 1 by volume), which solubilized the porphyrins.…”

Section: Lead Determinationmentioning

confidence: 99%

Effect of lead on blood protoporphyrin levels of a group of ring teal ducks (Callonetta leucophrys)

et al. 1989

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This research determined the relationships between blood lead level and zinc protoporphyrin (ZnPROTO), protoporphyrin IX (PROTO), and free erythrocyte protoporphyrin (FEP) levels in a group of 18 ring teal ducks. Blood samples were drawn from two groups of ring teal ducks as part of the routine health maintenance program of the New York Zoological Park. One group of six teals had been exposed to what is believed to be lead-contaminated dust, while the second group of twelve teals was unexposed. Blood samples were analyzed for lead by flameless atomic absorption and for protoporphyrins by fluorescence. Blood lead level and log blood lead level had positive correlations with each of the protoporphyrins: the logarithmic correlations were better than the nonlogarithmic correlations, and PROTO correlated better than ZnPROTO. With one exception, PROTO levels were higher than ZnPROTO levels. The results suggest that PROTO, FEP, or ZnPROTO could serve as a biological indicator of lead poisoning in ring teal ducks.

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Micro-Scale Photofluorometric Determination of "Free Erythrocyte Porphyrin" (Protoporphyrin IX)

Cited by 125 publications

References 0 publications

Lead effect on blood pressure in moderately lead‐exposed male workers

Lead effect on blood pressure in moderately lead‐exposed male workers

Light‐dependent Generation of Superoxide From Human Erythrocytes

Effect of lead on blood protoporphyrin levels of a group of ring teal ducks (Callonetta leucophrys)

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