MicroRNA‐145 regulates disabled‐2 and Wnt3a expression in cardiomyocytes under hyperglycaemia

Abstract

Aims: MicroRNA-145 (miR-145) could protect cardiomyocyte apoptosis against oxidative stress and repair infarcted myocardium. Angiotensin II (Ang II), a proinflammatory cytokine could modulate myocardial remodelling. However, the role of hyperglycaemia on miR-145 expression in cardiomyocyte or diabetes is not known. The effect of Ang II on miR-145 expression under hyperglycaemia in cardiomyocytes remains unknown. We sought to investigate the effect of hyperglycaemia and Ang II on miR-145 expression in cardiomyocytes. Methods: Rat cardiomyocytes were cultured under high glucose concentration (25 mmol/L), and streptozotocin-induced diabetic rats were established. TaqMan

Long non-coding RNA MEG3 serves as a ceRNA for microRNA-145 to induce apoptosis of AC16 cardiomyocytes under high glucose condition

“…In this study, the results of bioinformatics analysis predicted that miR-145 might contain the binding sites with MEG3, and this prediction was further proved by the experimental validation. Consistent with the findings of Wang et al [17], we also observed the reduction of miR-145 expression in HG-treated AC16 cells, and this reduction was overturned by MEG3 knockdown.…”

MicroRNA-377 Inhibits Atherosclerosis by Regulating Triglyceride Metabolism Through the DNA Methyltransferase 1 in Apolipoprotein E-Knockout Mice

“…Recently, miR-145 was reported to be associated with hyperglycemia in diabetes. 36 Thus, reduced plasma miR-145 levels observed in our patient samples may be related to increased fasting blood glucose levels. In contrast, miR-410 may be more closely related to plasma cholesterol level.…”

The complex role of Wnt ligands in type 2 diabetes mellitus and related complications

“…Compared to its homologue, Wnt3a is known to regulate the pathogenesis of T2DM from several processes but its role is controversial. Under the similar hyperglycaemia condition in STZ‐induced diabetic rat, Wnt3a/β‐catenin pathway was found to be inhibited in cardiomyocytes, 43 but swimming exercise was proved to alleviate insulin resistance through inhibiting the overactivated Wnt3a/β‐catenin pathway in skeletal muscles. 44 Wnt3a is an important modulator of the differentiation and maturation of β‐cells, overexpressing Wnt3a promotes the proliferation of porcine pancreatic stem cells (PSCs), which are valuable in transplantation application of T2DM.…”