MicroRNA‐155‐5p is associated with oral squamous cell carcinoma metastasis and poor prognosis

Abstract

Background Abnormal miRNA expression was recently implicated in the metastasis of oral squamous cell carcinoma (OSCC) and with a poor prognosis. The initiation of the invasion‐metastasis cascade involves epithelial‐mesenchymal transition (EMT). Our aim was to clarify how miRNA, especially miR‐155‐5p misexpression contributes to OSCC metastasis through EMT. Methods We collected tumor samples from 73 subjects with OSCC. The samples were analyzed by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR), and correlations between miR‐155‐5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by miRNA microarray and by transfection with a miR‐155‐5p mimic or inhibitor, followed by proliferation and wound‐healing migration assays. qRT‐PCR analyses of EMT makers in cells transfected with miR‐155‐5p inhibitor were performed. Results We found high miR‐155‐5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC‐3 cells also strongly expressed miR‐155‐5p. The epithelial marker E‐cadherin was strongly expressed in HSC‐3 cells transfected with miR‐155‐5p inhibitor, and we observed elevated SOCS1 and decreased STAT3 expression in these cells. Conclusions Our results suggest that miR‐155‐5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC.

miR-155-5p Promotes Progression of Acute Respiratory Distress Syndrome by Inhibiting Differentiation of Bone Marrow Mesenchymal Stem Cells to Alveolar Type II Epithelial Cells

“…Baba et al [22] confirmed that microRNA-155-5p is upregulated in oral squamous cell carcinoma and is correlated with tumor malignancy. It promotes epithelial mesenchymal transformation (EMT) by increasing SOCS1 and decreasing STAT3 expressions, which in turn promote tumor metastasis.…”

MicroRNA expression patterns in oral squamous cell carcinoma: hsa‐mir‐99b‐3p and hsa‐mir‐100‐5p as novel prognostic markers for oral cancer

“…16,24,69,81,82 Interestingly, Lerner et al and Scapoli et al found hsa-mir-155-5p to be downregulated in HNSCC and OSCC tissue. OS of oral squamous cell carcinoma was analyzed using Kaplan-Meier method.…”

“…OS of oral squamous cell carcinoma was analyzed using Kaplan-Meier method. 15,16,24,85 Furthermore, Rather et al have demonstrated that hsa-mir-155-5p overexpression is related to larger OSCC in a mouse model. HRs were calculated using a univariate cox regression hazard model, and P values were calculated using a log-rank test.…”

“…HRs were calculated using a univariate cox regression hazard model, and P values were calculated using a log-rank test. 24,25,86 Interestingly, Shi et al found that the expression levels of hsa-mir-155-5p are also increased in inflammatory areal that typically surrounds the tumor and that this overexpression is also correlated with a poorer survival. HR, hazard ratio; miRNA, MicroRNA; OS, Overall survival; TCGA, The Cancer Genome Atlas [Color figure can be viewed at wileyonlinelibrary.com] 4.8 | hsa-mir-155-5p hsa-mir-155-5p is one of the most frequently described miRNAs in OSCC and HNSCC cohorts.…”

“…[15][16][17][18] In addition, the upregulation of hsa-mir-21-5p and of hsa-mir-155-5p have been described as diagnostic and prognostic markers in non-European HNSCC cohorts. [19][20][21][22][23][24][25] However, to the best of our knowledge, the prognostic impact of hsa-mir-21-5p and hsa-mir-155-5p in European OSCC cohorts is still unexplored.…”