Micro<scp>RNA</scp> biogenesis factor <scp>DRB</scp>1 is a phosphorylation target of mitogen activated protein kinase <scp>MPK</scp>3 in both rice and Arabidopsis

Badmi, Raghuram; Sheikh, Arsheed Hussain; Rustagi, Yashika; Sinha, Alok

doi:10.1111/febs.13159

Cited by 74 publications

(76 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After this initial complex is formed, HYL1 and RCF3 might join the complex, a step dependent on both proteins' phosphorylation status, and anchor the precursors by specific RNA and protein interactions (3,26). Both HYL1 and RCF3 are recruited to the complex in their hypophosphorylated isoforms: states that are reached due to the CPL1/2 phosphatase activity and antagonized by MPK3 (3,26,28). Localization experiments revealed that CPL1 activity is required for the correct localization of RCF3 and HYL1 (3,21).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1

Karlsson

Christie

Seymour

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).

show abstract

Section: Discussionmentioning

confidence: 99%

“…The miRNA biogenesis cofactor HYL1 is subject to phosphorylation, which regulates its activity (3,28). HYL1 phosphorylation depends on CPL1, CPL2, and MITOGEN-ACTIVATED PROTEIN KINASE 3 (MPK3).…”

Section: Rcf3 Regulates Mirna Biogenesis Predominantly In Specific Plantmentioning

confidence: 99%

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1

Karlsson

Christie

Seymour

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…RNA extraction and Real time expression analysis was performed41. 2 −ΔΔC T values were calculated by method described in ref.…”

Section: Methodsmentioning

confidence: 99%

Functional Involvement of a Mitogen Activated Protein Kinase Module, OsMKK3-OsMPK7-OsWRK30 in Mediating Resistance against Xanthomonas oryzae in Rice

Jalmi

Sinha

2016

Sci Rep

View full text Add to dashboard Cite

Mitogen-activated protein kinases (MAPKs) are highly conserved signaling modules in eukaryotes, transmitting signals from upstream receptor to downstream target by phosphorelay mechanism. Here we report involvement of a poorly characterized group C MAPK of rice namely, OsMPK7 along with its upstream MAPK kinase, OsMKK3 and downstream target, OsWRKY30 during Xanthomonas oryzae infection, a causal agent of leaf blight disease in rice. X. oryzae infection resulted in induction of OsMPK7 and OsMKK3. OsMKK3 was found to physically interact and phosphorylate OsMPK7. Overexpression of OsMPK7 and OsMKK3, individually and in combinations resulted in inhibition of disease symptoms caused by X. oryzae, however silencing of OsMPK7 resulted in disease susceptibility. Furthermore, OsWRKY30 was identified as downstream target of OsMPK7 through protein-protein interaction techniques and was found to be a positive regulator of defence response against X. oryzae pathogen. The overexpression of OsMKK3-OsMPK7 upregulated genes involved in pathogenesis, cell wall structure maintenance and cell metabolism indicating possible mechanism of disease resistance. These leaves also showed restricted movement of the pathogen from the point of infection to uninfected area. Taken together, this work suggests a positive involvement of OsMKK3-OsMPK7-OsWRKY30 module in imparting disease resistance against X. oryzae infection in rice.

show abstract

“…Protein modifications also regulate the activity of the DCL1 complex. For example, HYL1 activity is controlled by phosphorylation and dephosphorylation (30,31). Furthermore, the expression levels of the DCL1 complex are regulated at transcriptional and posttranscriptional levels to influence miRNA accumulation (32)(33)(34).…”

mentioning

confidence: 99%

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Liu

Zhang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis. A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using primiR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5′ arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.

show abstract

MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase MPK3 in both rice and Arabidopsis

Cited by 74 publications

References 31 publications

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1

Functional Involvement of a Mitogen Activated Protein Kinase Module, OsMKK3-OsMPK7-OsWRK30 in Mediating Resistance against Xanthomonas oryzae in Rice

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Contact Info

Product

Resources

About