Micro‐sequence analysis of peptides and proteins using 4‐NN‐dimethylaminoazobenzene 4′‐isothiocyanate/phenylisothiocyanate double coupling method

The complete amino‐acid sequence of anglerfish somatostatin‐28 II

“…The exact biological function of this Secondly, the peptide was digested with endolysine which generates four fragments corresponding to cleavage at Lysr4, Lys18, LYS~~ and the fragments were analyzed for their N-terminal end by three successive cycles of the Chang double coupling method [13]. Ser', Ala15, Asnrg, Gly24 then Va12, Gly16, Phe2' and Phe25, then Asp3, Tyr21 and Thr26 were found in the successive cycles.…”

Atypical human liver alcohol dehydrogenase: the β2‐Bern subunit has an amino acid exchange that is identical to the one in the β2‐Oriental chain

“…Manual sequence degradations were performed by the DABITC-Edman method [20] utilizing byproducts to assist the identifications [2 11. Liquid phase sequencer degradataions were performed in a Beckman 890D sequencer with a 0.1 M Quadrol peptide program and with glycine-precycled polybrene as carrier [22].…”

Identification of the active site cysteine and of the disulfide bonds in the N‐terminal part of the molecule of bovine spleen cathepsin B

“…The N-terminal amino acids were determined as dansyl or DABTH derivatives [ 12,13]. Manual degradation was by the DABITC-Edman procedure [ 13 ].…”

“…Manual degradation was by the DABITC-Edman procedure [ 13 ]. The automatic version of the Edman degradation [14] was effected in Beckman 890 C Amino Acid Sequenator.…”