Micro-strains in the extracellular matrix induce angiogenesis

Sewell-Loftin, Mary Kathryn; Katz, Joshua B.; George, Steven C.; Longmore, Gregory D.

doi:10.1039/d0lc00145g

Cited by 23 publications

(30 citation statements)

References 69 publications

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“…In this study, sustained local lactate release from biodegradation of PLA and PLGA lead to reparative angiogenesis in superficial and ischemic wounds, and a similar effect may be at synergistic play in this scenario. In addition, mechanical perturbations and microstresses induced by the penetration and/or presence of flock fibers in the wound may trigger fiber density-dependent angiogenesis [ 58 , 59 ]. Granulation tissue formation, a hallmark in wound healing, is often subjectively measured [ 60 , 61 ].…”

Section: Resultsmentioning

confidence: 99%

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

McCarthy

Avegnon

Holubeck

et al. 2021

Materials Today Bio

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Electrostatic flocking is a textile technology that employs a Coulombic driving force to launch short fibers from a charging source towards an adhesive-covered substrate, resulting in a dense array of aligned fibers perpendicular to the substrate. However, electrostatic flocking of insulative polymeric fibers remains a challenge due to their insufficient charge accumulation. We report a facile method to flock electrostatically insulative poly(ε-caprolactone) (PCL) microfibers (MFs) and electrospun PCL nanofiber yarns (NFYs) by incorporating NaCl during pre-flock processing. Both MF and NFY were evaluated for flock functionality, mechanical properties, and biological responses. To demonstrate this platform's diverse applications, standalone flocked NFY and MF scaffolds were synthesized and evaluated as scaffold for cell growth. Employing the same methodology, scaffolds made from poly(glycolide-co- l -lactide) (PGLA) (90:10) MFs were evaluated for their wound healing capacity in a diabetic mouse model. Further, a flock-reinforced polydimethylsiloxane (PDMS) disc was fabricated to create an anisotropic artificial vertebral disc (AVD) replacement potentially used as a treatment for lumbar degenerative disc disease. Overall, a salt-based flocking method is described with MFs and NFYs, with wound healing and AVD repair applications presented.

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Section: Resultsmentioning

confidence: 99%

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

McCarthy

Avegnon

Holubeck

et al. 2021

Materials Today Bio

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show abstract

“…Subsequently, the HLECs were stimulated by IL-6 to secrete VEGF, which caused the HUVECs to grow toward the cancer cell clusters near the lymph channel as shown in Figures 4 , 5 . Even though the LTB chip showed recruitment of blood vessel cells in the chip, morphology of HUVECs in the angiogenesis process has to form the microvascular structure (Ko et al, 2019 ; Sewell-Loftin et al, 2020 ). It may happen by forming a small niche between the hydrogel block and bottom substrate during the gelation process.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic System to Analyze the Effects of Interleukin 6 on Lymphatic Breast Cancer Metastasis

Cho

Choi

Kim

et al. 2021

Front. Bioeng. Biotechnol.

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Metastasis is the primary cause of a large number of cancer-associated deaths. By portraying the precise environment of the metastasis process in vitro, the microfluidic system provides useful insights on the mechanisms underlying cancer cell migration, invasion, colonization, and the procurement of supplemental nutrients. However, current in vitro metastasis models are biased in studying blood vessel-based metastasis pathways and thus the understanding of lymphatic metastasis is limited which is also closely related to the inflammatory system. To understand the effects of inflammatory cytokines in lymphatic metastasis, we developed a three-channel microfluidic system by mimicking the lymph vessel-tissue-blood vessel (LTB) structure. Based on the LTB chip, we successfully confirmed the inflammatory cytokine, interleukin 6 (IL-6), -mediated intercellular communication in the tumor microenvironment during lymphatic metastasis. The IL-6 exposure to different subtypes of breast cancer cells was induced epithelial-mesenchymal transition (EMT) and improved tissue invasion property (8-fold). And the growth of human vein endothelial cells toward the lymph vessel channel was observed by VEGF secretion from human lymphatic endothelial cells with IL-6 treatment. The proposed LTB chip can be applied to analyze the intercellular communication during the lymphatic metastasis process and be a unique tool to understand the intercellular communication in the cancer microenvironment under various extracellular stimuli such as inflammatory cytokines, stromal reactions, hypoxia, and nutrient deficiency.

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“…Therefore, this model cannot reflect vital structures and microenvironments that usually occur under specific physiological or pathological conditions. For example, in tumor development, microenvironmental factors such as blood and lymphatic system ( Mazzone and Bergers, 2019 ), surrounding fibroblasts ( Hussain et al, 2020 ), and mechanical stimulation ( Sewell-Loftin et al, 2020 ) affect the behavior and molecular characteristics of cancer cells and thus change the invasive ability of cancer, resistance to chemotherapy, and growth in vivo . Given these main limitations, traditional cell line disease models often misestimate the disease development and treatment response in vivo , which seriously limits the application of cell line models in experimental research.…”

Section: Introductionmentioning

confidence: 99%

Research Progress, Challenges, and Breakthroughs of Organoids as Disease Models

Huang

Tang

et al. 2021

Front. Cell Dev. Biol.

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Traditional cell lines and xenograft models have been widely recognized and used in research. As a new research model, organoids have made significant progress and development in the past 10 years. Compared with traditional models, organoids have more advantages and have been applied in cancer research, genetic diseases, infectious diseases, and regenerative medicine. This review presented the advantages and disadvantages of organoids in physiological development, pathological mechanism, drug screening, and organ transplantation. Further, this review summarized the current situation of vascularization, immune microenvironment, and hydrogel, which are the main influencing factors of organoids, and pointed out the future directions of development.

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Micro-strains in the extracellular matrix induce angiogenesis

Abstract:
A microtissue platform with independent control over biomechanical parameters is described and used to demonstrate how cell-generated forces drive angiogenesis.

Cited by 23 publications

References 69 publications

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

Microfluidic System to Analyze the Effects of Interleukin 6 on Lymphatic Breast Cancer Metastasis

Research Progress, Challenges, and Breakthroughs of Organoids as Disease Models

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Micro-strains in the extracellular matrix induce angiogenesis

Abstract: A microtissue platform with independent control over biomechanical parameters is described and used to demonstrate how cell-generated forces drive angiogenesis.

Cited by 23 publications

References 69 publications

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

Electrostatic flocking of salt-treated microfibers and nanofiber yarns for regenerative engineering

Microfluidic System to Analyze the Effects of Interleukin 6 on Lymphatic Breast Cancer Metastasis

Research Progress, Challenges, and Breakthroughs of Organoids as Disease Models

Contact Info

Product

Resources

About

Abstract:
A microtissue platform with independent control over biomechanical parameters is described and used to demonstrate how cell-generated forces drive angiogenesis.