Microaerophilic environments improve the productivity of medium chain length polyhydroxyalkanoate biosynthesis from fatty acids in Pseudomonas putida LS46

Blunt, Warren; Dartiailh, Christopher; Sparling, Richard; Gapes, Daniel J.; Levin, David B.; Çiçek, Nazim

doi:10.1016/j.procbio.2017.04.028

Cited by 18 publications

(24 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strain used in this study was Pseudomonas putida LS46 [35]. Strain culturing and maintenance procedures were as specified previously [27]. Ramsay's minimal medium [36] was used in all studies.…”

Section: Micro-organism Medium and Substratesmentioning

confidence: 99%

“…A 7 L autoclavable glass reactor was used (Applikon, Foster City, CA, USA) with an initial working volume of 3 L. The reactor setup, sensor calibration, and sterilization procedures were identical to those previously described [27]. Experiments were initiated with the addition of a 5% (vol vol −1 ) inoculum from an overnight culture of Ramsay's medium with 20 mM octanoic acid (9.5 mL into 3 L) as the carbon source.…”

Section: Reactor Setup and Operationmentioning

confidence: 99%

“…The carbon balance was performed assuming that all consumed carbon could be accounted for through measured CO 2 , PHA biomass (X PHA ), and non-PHA residual cell mass (X r ). The mass balance followed the same approach described previously [26,27].…”

Section: Measurement Of Co 2 and Mass Balancingmentioning

confidence: 99%

“…Previously, we have shown that in the production of mcl-PHAs from medium and long chain fatty acids, imposing O 2 limitation on the culture results in a significant redirection of carbon flux toward PHA synthesis [26,27]. However, the aforementioned studies used batch or simple fed-batch strategies as proof-of-concept, and overall productivities were relatively low.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46

et al. 2019

Self Cite

View full text Add to dashboard Cite

High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements as feedback variables) was used to control an oxygen-limited fed-batch process for improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset of oxygen limitation half-way through the process (14 h post inoculation), 28.8 ± 3.9 g L−1 total biomass (with PHA content up to 61 ± 8% cell dry mass) was reliably achieved within 27 h using octanoic acid as the carbon source in a bench-scale (7 L) bioreactor operated under atmospheric conditions. This resulted in a final volumetric productivity of 0.66 ± 0.14 g L−1 h−1. Delivering carbon to the bioreactor as a continuous drip feed process (a proactive feeding strategy compared to pulse feeding) made little difference on the final volumetric productivity of 0.60 ± 0.04 g L−1 h−1. However, the drip feed strategy favored production of non-PHA residual biomass during the growth phase, while pulse feeding favored a higher rate of mcl-PHA synthesis and yield during the storage phase. Overall, it was shown that the inherent O2-limitation brought about by HCD cultures can be used as a simple and effective control strategy for mcl-PHA synthesis from fatty acids. Furthermore, the pulse feed strategy appears to be a relatively easy and reliable method for rapid optimization of fed-batch processes, particularly when using toxic substrates like octanoic acid.

show abstract

Section: Micro-organism Medium and Substratesmentioning

confidence: 99%

Section: Reactor Setup and Operationmentioning

confidence: 99%

Section: Measurement Of Co 2 and Mass Balancingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The exploitation of polymers production was assessed and described in the last two papers of this special section. Blunt et al optimized the conditions of oxidative synthesis of medium chain length polyhydroxyalkanoate from fatty acids [15]. Yim et al developed a new type of eco-friendly and sustainable fabric by using tea as nitrogen source for bacterial cellulose production [16].…”

mentioning

confidence: 99%

Foreword: Advances in “Polymer/Fibre Biotechnology”

Silva

Cavaco-Paulo

2017

Process Biochemistry

View full text Add to dashboard Cite

Biotechnology has been applied as a valuable tool to produce useful bio-based products from non-petrochemical renewable resources. Biologically produced polymers present advantages related with the biodegradability, performance, cheapness of substrate and defined structural variability. Beyond that, enzymes, the most proficient catalysts, continue to offer the most competitive processes compared with the chemical ones. Advances in protein engineering technology and the environmental and economic requirements contribute for the continuous search of acceptable biotechnological solutions for the areas, including, polymer and textile, medical, pharmaceutical, among others. New trends and strategies have been highlighted in recent studies leading to significant advances in enzyme biocatalysis.

show abstract

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Vermeer

Depaz

Berg

et al. 2022

Biotech & Bioengineering

View full text Add to dashboard Cite

Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On the basis of analysis of the metabolic pathways, the hypothesis was formulated that mcl-PHA production is more favorable under oxygen-limited conditions than short-chain-length PHA (scl-PHA). This hypothesis was confirmed by bioreactor experiments showing that oxygen limitation during the PHA accumulation experiments resulted in a higher fraction of mcl-PHA over scl-PHA (i.e., a PHA content

show abstract

Microaerophilic environments improve the productivity of medium chain length polyhydroxyalkanoate biosynthesis from fatty acids in Pseudomonas putida LS46

Cited by 18 publications

References 48 publications

Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46

Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46

Foreword: Advances in “Polymer/Fibre Biotechnology”

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Contact Info

Product

Resources

About