Microalgae-based carbon sequestration: growth of spirulina platensis with flue gas feeding

Arata, S.; Strazza, Carlo; Lodi, Alessandra; Borghi, Adriana Del

doi:10.1016/j.jbiotec.2010.09.201

Cited by 1 publication

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“…Using CO 2 as a main source of carbon and light as sources of energy, microalgae are the most efficient photosynthetic organism among the green lineage. In addition, they can be cultivated on non‐arable land (such as deserts) and in variable climates, in non‐potable water, and can be harvested daily . Algal lipid content varies from 1 to 70% of DCW (dry cell weight), but can reach 90% under stress conditions (such as nitrogen deficiency, high salinity, intense luminosity) .…”

Section: Introductionmentioning

confidence: 99%

Rapid analysis and quantification of major neutral lipid species and free fatty acids by HPLC‐ELSD from microalgae

Donot

Strub

Fontana

et al. 2016

Euro J Lipid Sci & Tech

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A method based on normal-phase high performance liquid chromatography combined with evaporative light-scattering detector was developed to quantify major neutral lipids from microalgae. A binary gradient enabled a good resolution of hydrocarbons and major neutral lipids by classes in less than 13 min. A good linearity was observed from 0.9 to 30 mg. Limits of detection (LOD) and quantification (LOQ) was from 3 to 64 ng depending on the analyte. The intra-and inter-run precision (% RSD) of analysis was found less than 10%. The method was successfully applied to microalgae lipid extracts from Botryococcus braunii, Chlorella vulgaris, Spirulina platensis, and Porphyridium cruentum. B. braunii was distinguished by the highest levels of lipids, triacylglycerol (TAGs), and diacylglycerol (DAGs) being the major compounds.Practical applications: Neutral lipids produced by the microalgae are an interesting way for the development of biofuel from renewable carbon. A simple quantification of these lipids is important in the implementation of oleaginous microalgae screening as well as in the bioprocess optimization of algal lipid production. Besides, determining quantitatively the major lipid classes provides information in the understanding of the biosynthetic pathways and the accumulation of these lipids in relation with algal culture conditions.

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Section: Introductionmentioning

confidence: 99%