Microalgae fiber optic biosensors for herbicide monitoring using sol–gel technology

Peña‐Vázquez, Elena; Maneiro, Emilia; Pérez-Conde, C.; Moreno‐Bondi, María C.; Costas, Eduardo

doi:10.1016/j.bios.2009.05.013

Cited by 65 publications

(37 citation statements)

References 31 publications

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“…For instance Podola et al 1 demonstrated that the identication of a specic herbicide is in principle possible by employing an array chip algal biosensor based on nine different microalgal strains. Altamirano et al 2 and Peña-Vázquez et al 3 further suggested the use of mutants sensitive/resistant to a given toxicant to further increase the sensitivity and to improve the specicity of response patterns. During the last years, advances in cell immobilization strategies have provided the possibility to position different cells in microarrays by using various deposition and printing techniques, 4,5 such as micro-contact printing (microstamping) 6,7 and non-contact printing like ink-jet printing.…”

Section: Introductionmentioning

confidence: 99%

“…Microalgae are frequently used in bioassays as they are very sensitive towards different pollutants 35 such as heavy metals 36-38 and herbicides. 3,30,39 In the present study, the triazine herbicide atrazine was used as model substrate for short-term toxicity testing in aquatic samples. The phytotoxic action of this herbicide is based on the interference with the electron transport in the photosystem II (PSII) of photosynthetic organisms.…”

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confidence: 99%

“…Inhibition data (N ¼ 6) were used to model concentrationresponse curves using log-logistic analysis according eqn (3).…”

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confidence: 99%

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Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca 2+ -ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the potential of the hybrid matrix for the design of cell-based detection systems. The herbicide atrazine as well as copper ions have been used as model toxicants.Short-term toxicity tests (exposure time: 1 h) have been carried out using atrazine and changes in chlorophyll a (Chl a) fluorescence were measured by imaging pulse amplitude modulated-fluorometry (Imaging-PAM). C. vulgaris cells immobilized within alginate/silica hydrogels demonstrated a highly reproducible response pattern and compared well to freely suspended cells. Activity and response sensitivity of immobilized cells to atrazine was largely maintained for up to 8 weeks, especially when stored under cool conditions in the dark. Furthermore, immobilized cells could be repeatingly used for short-term toxicity tests as atrazine produces a reversible inhibition of photosynthesis.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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“…Multiple analytes (microcystin-Lr and trinitrotoluene) were detected using monoclonal antibodies against them on fiber optic cable (Long et al 2010 (Kumar et al 2006). Microalgae immobilized in solgel membranes coupled with bifurcated fiber optic cable and spectrofluorometer was used to detect herbicide simazine (Peña-Vázquez et al 2009). Verma et al (2010) used Bacillus badius whole cells with phenol red as an indicator coimmobilized onto circular plastic discs with sol-gel approach and a fiber optic transducer system to determine cadmium in milk by inhibition of urease produced by the bacteria.…”

Section: Immunoassay Basedmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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“…Operative specific herbicide biosensors were obtained by immobilizing microalgae strains on silicone dishes (Cellon TM ) (Orellana et al, 2007; or by encapsulating in silicate sol-gel matrices (Peña-Vázquez et al, 2009;. Such procedures allow us long term stability of cells as determined by a constant F'm values for at least 3 weeks.…”

Section: Operative Microalgae Biosensormentioning

confidence: 99%

Highly Specific Biosensors to Herbicides, Based on Sensitive- and Resistant-Mutants of Microalgae

Hernández-Allica¹,

Carrera-Martínez²,

López‐Rodas³

et al. 2011

Herbicides and Environment

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Microalgae fiber optic biosensors for herbicide monitoring using sol–gel technology

Cited by 65 publications

References 31 publications

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Optical biosensors for food quality and safety assurance—a review

Highly Specific Biosensors to Herbicides, Based on Sensitive- and Resistant-Mutants of Microalgae

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