Microalgae harvesting using ozoflotation: Effect on lipid and FAME recoveries

Velásquez-Orta, Sharon B.; García-Estrada, R.; Monje–Ramírez, Ignacio; Harvey, Adam P.; Ledesma, María Teresa Orta

doi:10.1016/j.biombioe.2014.08.022

Cited by 44 publications

(19 citation statements)

References 23 publications

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“…The authors suggested that the increase in lipids could partially be due to the CTAB promoting in situ cell lysis by solubilizing the phospholipid bilayer, thus increasing the amount of extractable lipid. Similar findings were demonstrated by Velasquez-Orta et al [107] when using ozoflotation to harvest microalgae. They reported that the amount of lipid extracted and FAME recovered from the biomass doubled when compared to biomass harvested by centrifugation.…”

Section: Flotationsupporting

confidence: 83%

“…They reported that the amount of lipid extracted and FAME recovered from the biomass doubled when compared to biomass harvested by centrifugation. In addition, the oxidative stability of biodiesel was enhanced by the effect that ozone had in the degree of FAME saturation [107]. In theory, flotation can be used to harvest and as a cell disruption method, for the improved extraction of lipids.…”

Section: Flotationmentioning

confidence: 99%

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Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants

et al. 2015

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Section: Flotationsupporting

confidence: 83%

Section: Flotationmentioning

confidence: 99%

Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants

et al. 2015

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“…and 0.14 mg O3/mg of biomass. Velasquez-Orta et al [41] obtained a biomass recovery of 79%, using higher doses of ozone (0.23 mg O3/mg of biomass) and lower microalgal concentration (420 mg SST/L) than in this study (0.16 mg O3/mg of dry biomass and a 500 mg SST /L biomass concentration).…”

Section: Microalgal Harvestingcontrasting

confidence: 38%

The effect of bubble size distribution on the release of microalgae proteins by ozone-flotation

Oliveira

Monje–Ramírez

Carissimi

et al. 2019

Separation and Purification Technology

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Interest in sustainable energy production is one of the most important resources in the development of technologies, and one of the promising sources is the use of microalgae as a raw material. These are obtained in nature, do not need good conditions to develop, occupy a small area for growth and replace the use of food products for the production of biofuel. However, one of the biggest hurdle is the biomass harvest and one of the innovative techniques being proposed is ozone-flotation. Flotation processes are influenced by the physical characteristics of bubbles and particles, and therefore it is necessary to investigate the size distribution of generated bubbles, their action in microalgal cells and the oxidation and release of proteins that act as biosurfactants. In this work the effect of ozone bubble size in a three-phase system (ozonewastewatermicroalgae) and size of microalgal particles separated by ozone-flotation were evaluated. Three ozone flows were used (0.2, 0.6 and 1 L/min). Best results were obtained with a flow of 0.6 L/min achieving 88.5% efficiency, increased biomass oxidation, destabilization of microalgal cells (zeta potential 3.17 mV), highest protein release (46.7 mg/L) and, a demonstrated efficiency of proteins in reducing bubble coalescence.Regarding the latter, no differences in size or distribution of the ozone bubbles were obtained between the 3 evaluated heights of the column (bottom, middle and top). At this flow the mean bubble diameters were maintained at 1910, 2028 and 2071 μm for bottom, middle and top, respectively, with approximately 50% of the bubbles concentrated up to 2000 μm. In addition, due to the higher action of ozone with microalgal cells, smaller particle sizes in the column were found due to the oxidizing action of ozone.

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“…cultivation in the treatment of wastewater for nutrient removal was evaluated. After cultivation, two techniques of harvesting were compared, coagulation-flocculation which is a very efficient and proven technique for harvesting microalgae (Das et al, 2016;Reyes and Labra, 2016;Vandamme et al, 2013) and ozone-flotation, which is currently being developed (Cheng et al, 2011;Komolafe et al, 2014;Valeriano González et al, 2016;Velasquez-Orta et al, 2014;Orta Ledesma de Velásquez et al, 2017). The evaluation for both processes was performed by determining nutrient removal at different water processing stages: 1) after treatment by Scenedesmus, and 2) after recovering the biomass.…”

Section: Introductionmentioning

confidence: 99%

Comparison between coagulation-flocculation and ozone-flotation for Scenedesmus microalgal biomolecule recovery and nutrient removal from wastewater in a high-rate algal pond

Oliveira

Carissimi

Monje–Ramírez

et al. 2018

Bioresource Technology

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The removal of nutrients by Scenedesmus sp. in a high-rate algal pond, and subsequent algal separation by coagulation-flocculation or flotation with ozone to recover biomolecules, were evaluated. Cultivation of Scenedesmus sp. in wastewater resulted in complete NH-H removal, plus 93% total nitrogen and 61% orthophosphate removals. Ozone-flotation obtained better water quality results than coagulation-flocculation for most parameters (NH-N, NTK, nitrate and nitrite) except orthophosphate. Ozone-flotation, also produced the highest recovery of lipids, carbohydrates and proteins which were 0.32 ± 0.03, 0.33 ± 0.025 and 0.58 ± 0.014 mg/mg of biomass, respectively. In contrast, there was a low lipid extraction of 0.21 mg of lipids/mg of biomass and 0.12-0.23 mg of protein/mg of biomass in the coagulation-flocculation process. In terms of biomolecule recovery and water quality, ozone showed better results than coagulation-flocculation.

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Microalgae harvesting using ozoflotation: Effect on lipid and FAME recoveries

Abstract: This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License ePrints-Newcastle University ePrints http://eprint.ncl.ac.uk

Cited by 44 publications

References 23 publications

Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants

Harvesting of microalgae within a biorefinery approach: A review of the developments and case studies from pilot-plants

The effect of bubble size distribution on the release of microalgae proteins by ozone-flotation

Comparison between coagulation-flocculation and ozone-flotation for Scenedesmus microalgal biomolecule recovery and nutrient removal from wastewater in a high-rate algal pond

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