Microalgal culture in animal cell waste medium for sustainable ‘cultured food’ production

Haraguchi, Yuji

doi:10.1007/s00203-021-02509-x

Cited by 14 publications

(10 citation statements)

References 37 publications

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“…Therefore, seawater or euryhaline microalgae 18 rather than freshwater microalgae are suitable for this system. In this study, seawater microalgae, C. littorale , were selected based on previous data (Haraguchi and Shimizu 2021 ). Because mammalian cells and microalgae hardly consume inorganic salts, such as sodium, calcium, and potassium, during their cultivation (Haraguchi and Shimizu 2021 ), these inorganic salts need not be added until several cycles of CCC are completed.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously reported that mammalian cells can be cultivated using nutrients extracted from microalgae (Okamoto et al 2020 and 2022 ). In addition, we have also demonstrated successful microalgal culture in mammalian cell waste medium (Haraguchi and Shimizu 2021 ). In this study, we aimed to demonstrate an unprecedented circular cell culture (CCC) system that combines mammalian muscle cell culture with microalgal nutrients and microalgal culture with mammalian muscle cell waste medium as a more advanced and sustainable technology for cultured meat production.…”

Section: Introductionmentioning

confidence: 93%

“…The second issue associated with culture media is the mode of waste treatment. While mammalian myoblasts vigorously deplete the glucose and glutamine in the culture media, only 26% of other amino acids are consumed (Haraguchi and Shimizu 2021 ). In addition, the amounts of pyruvate, vitamins, and inorganic salts in the waste media after the culturing step remain unchanged (Haraguchi and Shimizu 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…While mammalian myoblasts vigorously deplete the glucose and glutamine in the culture media, only 26% of other amino acids are consumed (Haraguchi and Shimizu 2021 ). In addition, the amounts of pyruvate, vitamins, and inorganic salts in the waste media after the culturing step remain unchanged (Haraguchi and Shimizu 2021 ). In contrast, the amount of ammonia, which adversely affects mammalian cells, increases after culturing (Schneider et al 1996 ; Price 2017 ; Haraguchi and Shimizu 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the amounts of pyruvate, vitamins, and inorganic salts in the waste media after the culturing step remain unchanged (Haraguchi and Shimizu 2021 ). In contrast, the amount of ammonia, which adversely affects mammalian cells, increases after culturing (Schneider et al 1996 ; Price 2017 ; Haraguchi and Shimizu 2021 ). Generally, the waste medium is discarded and replaced with a new culture medium because of the reduction in nutrients and accumulation of waste products.…”

Section: Introductionmentioning

confidence: 99%

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A circular cell culture system using microalgae and mammalian myoblasts for the production of sustainable cultured meat

2022

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For sustainable production of cultured meat, we propose a novel circular cell culture (CCC) system in which microalgae are used as nutrient supply for the mammalian cell culture and as a waste-medium recycler. Chlorococcum littorale , RL34 hepatocytes, and C2C12 myoblasts were used as cell sources for microalgae, growth factor-producing cells, and muscle cells, respectively. In the first cycle, C2C12 cells were amplified 4.0-fold after 48 h of culture in an RL34 cell-conditioned medium. In the second cycle, C2C12 cells were cultured in the C. littorale culture waste medium to which the C. littorale -derived nutrients were added. The proliferation rates of C. littorale and C2C12 and the nutrient extraction efficiency from C. littorale were the same in the first and second cycles. Therefore, this CCC system, which works without additional grain-derived nutrients and animal sera, will help drastically reduce environmental load, resource/energy consumption, and costs in future cultured meat production. Supplementary Information The online version contains supplementary material available at 10.1007/s00203-022-03234-9.

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Section: Discussionmentioning

confidence: 99%