Microalgal diversity fosters stable biomass productivity in open ponds treating wastewater

Cho, Dae‐Hyun; Choi, Jung-Woon; Kang, Zion; Kim, Byung-Hyuk; Oh, Hee‐Mock; Kim, Hee‐Sik; Ramanan, Rishiram

doi:10.1038/s41598-017-02139-8

Cited by 68 publications

(40 citation statements)

References 41 publications

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“…In particular, large outdoor cultures of algae exhibit sudden catastrophic population “crashes” due to environmental fluctuations, disease, pests, and invasive species. Several studies have demonstrated the feasibility of growing algal polycultures in open outdoor ponds (Beyter et al., ; Bhattacharjee & Siemann, ; Cho et al., ; Sturm, Peltier, Smith, & deNoyelles, ), but because these studies did not simultaneously evaluate the performance for each of those same species when grown as monocultures, it is not possible to isolate the effect of species richness (as opposed to species identity) on the performance of the cultures. As a result, the hypothesis that biodiversity improves several aspects of algal biofuel cultivation remains untested under outdoor conditions.…”

Section: Introductionmentioning

confidence: 99%

Biodiversity improves the ecological design of sustainable biofuel systems

et al. 2018

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For algal biofuels to become a commercially viable and sustainable means of decreasing greenhouse gas emissions, growers are going to need to design feedstocks that achieve at least three characteristics simultaneously as follows: attain high yields; produce high quality biomass; and remain stable through time. These three qualities have proven difficult to achieve simultaneously under the ideal conditions of the laboratory, much less under field conditions (e.g., outdoor culture ponds) where feedstocks are exposed to highly variable conditions and the crop is vulnerable to invasive species, disease, and grazers. Here, we show that principles from ecology can be used to improve the design of feedstocks and to optimize their potential for "multifunctionality." We performed a replicated experiment to test these predictions under outdoor conditions. Using 80 ponds of 1,100 L each, we tested the hypotheses that polycultures would outperform monocultures in terms of the following functions: biomass production, yield of biocrude from biomass, temporal stability, resisting population crashes, and resisting invasions by unwanted species. Overall, species richness improved stability, biocrude yield, and resistance to invasion. While this suggests that polycultures could outperform monocultures on average, invasion resistance was the only function where polycultures outperformed the best single species in the experiment. Due to tradeoffs among different functions that we measured, no species or polyculture was able to maximize all functions simultaneously. However, diversity did enhance the potential for multifunctionality-the most diverse polyculture performed more functions at higher levels than could any of the monocultures. These results are a key finding for ecological design of sustainable biofuel systems because they show that while a monoculture may be the optimal choice for maximizing short-term biomass production, polycultures can offer a more stable crop of the desired species over longer periods of time.

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Section: Introductionmentioning

confidence: 99%

Biodiversity improves the ecological design of sustainable biofuel systems

et al. 2018

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“…The determinations were corroborated by the Hach kits (Hach, Loveland, CO, USA), per standard methods 10127, 8039 and 8507 for the determination total phosphorus, nitrates and nitrites, respectively. Ammonium was measured using Hach kits (Hach, Loveland, CO, USA), as per standard methods 8155. pH was measured using a Fisher-Scientific probe ( Ben et al 2008 , Cho et al 2017 , Colombo et al 2017 , Stauch-White et al 2017 )…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Roque¹,

Pérez-Luna²,

Acosta³

et al. 2018

BDJ

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As Chiapas state, México, counts on an extensive hydrography with diverse nutrimental and climatic characteristics, it therefore allows isolating and identifying microalgae with bioenergetics potential.For this purpose, samples from 8 locations were collected, corresponding to 6 rivers, a wastewater and a springwater. The isolation of microalgae was developed for 4 weeks with 12:12 light/dark cycles. We demonstrated that the most efficient means for the isolation of microalgae of the hydrographic areas evaluated was the medium BG11 with 80.53% effectiveness. Of the microalgal consortium identified, 90% are composed of microalgae belonging to the class Chlorophycear. It was shown that another factor favouring the richness of morphotypes identified in the Santo Domingo River is associated with adequate concentrations of macroelements such as nitrates, nitrites, ammonium, phosphorus, sodium, potassium, magnesium and calcium at concentrations of 0.03 mg/l, 0.0006 mg/l, 0.08 mg/l, 0.03 mg/l, 62.93 mg/l, 5.46 mg/l, 34.52 mg/l and 48.78 mg/l respectively and microelements such as copper, zinc, iron, andmanganese at concentrations less than 0.2 mg/l in all microelements. The identified morphotypes, according to literature, have lipid contents ranging from 2 to 90%; this is of biotechnological importance for the production of biodiesel.

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“…To overcome the poor biomass accumulation, open ponds that mimic natural environments of the microalgae are alternative option for the most commercial microalgae [5]. These cultivation systems present relatively lower construction and operating costs and large ones can be constructed on nonagricultural lands [6].…”

Section: Introductionmentioning

confidence: 99%

Systems biology approach identifies functional modules and regulatory hubs related to secondary metabolites accumulation after transition from autotrophic to heterotrophic growth condition in microalgae

2020

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Heterotrophic growth mode is among the most promising strategies put forth to overcome the low biomass and secondary metabolites productivity challenge. To shedding light on the underlying molecular mechanisms, transcriptome meta-analysis was integrated with weighted gene co-expression network analysis (WGCNA), connectivity analysis, functional enrichment, and hubs identification. Meta-analysis and Functional enrichment analysis demonstrated that most of the biological processes are up-regulated at heterotrophic growth condition, which leads to change of genetic architectures and phenotypic outcomes. WGNCA analysis of meta-genes also resulted four significant functional modules across logarithmic (LG), transition (TR), and production peak (PR) phases. The expression pattern and connectivity characteristics of the brown module as a non-preserved module vary across LG, TR, and PR phases. Functional analysis identified Carotenoid biosynthesis, Fatty acid metabolism and Methane metabolism as enriched pathways in the non-preserved module. Our integrated approach was applied here, identified some hubs, such as a serine hydroxymethyltransferase (SHMT1), which is the best candidate for development of metabolites accumulating strains in microalgae. Current study provided a new insight into underlying metabolite accumulation mechanisms and opens new avenue for the future applied studies in the microalgae field. OPEN ACCESSCitation: Panahi B, Farhadian M, Hejazi MA (2020) Systems biology approach identifies functional modules and regulatory hubs related to secondary metabolites accumulation after transition from autotrophic to heterotrophic growth condition in microalgae. PLoS ONE 15(2): e0225677. https://

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Microalgal diversity fosters stable biomass productivity in open ponds treating wastewater

Cited by 68 publications

References 41 publications

Biodiversity improves the ecological design of sustainable biofuel systems

Biodiversity improves the ecological design of sustainable biofuel systems

Evaluation of the population dynamics of microalgae isolated from the state of Chiapas, Mexico with respect to the nutritional quality of water

Systems biology approach identifies functional modules and regulatory hubs related to secondary metabolites accumulation after transition from autotrophic to heterotrophic growth condition in microalgae

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