Microalgal Systems Biology Through Genome-Scale Metabolic Reconstructions for Industrial Applications

Hong, Seong-Joo; Lee, Choul‐Gyun

doi:10.1016/b978-0-12-800776-1.00023-6

Cited by 5 publications

(2 citation statements)

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“…This approach is also used to investigate the molecular mechanisms underlying the adaptation of algae from diverse habitats to various biotic and abiotic stress factors (Sun et al ). As summed up by Hong and Lee (), transcriptomic analyses are essential for systems biology because de novo and comparative transcriptomic analyses can provide a framework for metabolic reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Optimization of high quality total RNA isolation from the microalga, Chlorella sp. (Trebouxiophyceae, Chlorophyta) for next‐generation sequencing

Poong

Lim

Lai

et al. 2017

Phycological Research

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The advent of high-throughput next-generation sequencing (NGS) has enabled more comprehensive transcriptome analyses to obtain gene expression data and understand metabolic pathways essential for functional analysis and systems biology. The isolation of high purity and intact RNA of sufficient quality and yield is crucial to the success of NGS sequencing. In green microalgae, extraction of nucleic acids is hindered by high concentration of lipids and polysaccharides that co-precipitate with nucleic acids thus resulting in reduced yield and poor quality extracts. The present study compared the performance of standard and modified total RNA isolation protocols using different cell disruption techniques combined with TRIzol reagent or a commercially available kit, in meeting the stringent requirements for downstream NGS application of a green freshwater microalga, Chlorella sp. The protocols were evaluated for (i) the yield of RNA, (ii) integrity of RNA as determined by the RNA integrity number and (iii) the purity of RNA as determined by the A 260 /A 280 and A 260 /A 230 ratios. In general, higher yields were obtained by cell disruption via flash freezing and homogenizing with liquid nitrogen followed by lysis with TRIzol reagent. We recommend the incorporation of a salt precipitation step to improve the purity and integrity of RNA isolated. Results of this study served as a simple and low-cost practical guide for researchers working on isolation of high quality total RNA from microalgae, considering that most relevant publications do not provide a detailed methodology for total RNA isolation or use more expensive methods (e.g. bead-beater).

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Section: Introductionmentioning

confidence: 99%

Optimization of high quality total RNA isolation from the microalga, Chlorella sp. (Trebouxiophyceae, Chlorophyta) for next‐generation sequencing

Poong

Lim

Lai

et al. 2017

Phycological Research

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“…galactose e xylose, na parede celular (Yokoyama e Honda, 2007;Hong et al, 2011;Hong e Lee, 2015). Em geral, as enzimas específicas para a sua digestão dos polissacarídeos não amiláceos estão ausentes ou presentes em baixas concentrações em diversas espécies de peixe (Yokoyama and Honda, 2007, Hong and Lee, 2015, Hong et al, 2011, Kuz'mina, 1996. No entanto, apesar das células da FSZ estarem íntegras, foi registrada uma boa utilização dos nutrientes do ingrediente, com CDAs acima de 80%.…”

Section: Farinha Deunclassified

Microalgas como ingrediente e suplemento dietético para tilápia <i>Oreochromis niloticus</i>: valor biológico, desempenho e composição da carcaça

Barone¹

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Microalgae as a dietary supplement for tilapia (Oreochromis niloticus): biological value, performance and carcass composition There is a great potential for expand the microalgae production as by the variety of it uses as for the products and processes which may derive from them. Amongst such uses, nutritional and functional characteristics provide them a high biological value for use in human and animal nutrition. Nevertheless, few studies have evaluated their potential for fish nutrition more specifically tilapia, which is one of the main aquaculture products in the world. The aim of this research was to evaluate the potential use of two microalgae and the optimal level of inclusion of Schizochytrium in diets for tilápia. Therefore, tests were conducted to evaluate the apparent digestibility coefficient (ADC) of microalgae with high protein content Chlorella sorokiniana and another with high energy, Schizochytrium sp. and also performance assay and feed digestibility containing inclusion levels (0%, 1%, 2.5%, 5%, 7.5% and 10%) of Schizochytrium in order to determine the optimum addition level. Both species were very digestible for tilapia with ADCs for protein of 90.51 and 97.20 and for energy 84.22 and 82.55 to Chlorella and Schizochytrium respectively. The increasing levels of inclusion of Schizochytrium changed the digestibility of feed for the protein, through a ADC from 88.07 to 86.01 and for energy from 74.19 to 67.35 ADC. In the growth test as the inclusion of Schizochytrium increased there was an increase in feed intake and feed conversion ratio but any other growth parameters were changed. Regarding the carcass composition, there were no changes in macronutrients composition however, the increasing levels of Schizochytrium altered the fatty acid profile by increasing the amount of omega 3 mainly docosahexaenoic acid (DHA) and reduced the n-6/n-3 ratio. Thus, it is possible to use the Schizochytrium to modify the fatty acid profile of tilápia by incorporating omega 3, with little loss in performance parameters

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Microalgae, a Biological Resource for the Future

Kim

2019

Essentials of Marine Biotechnology

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Microalgal Systems Biology Through Genome-Scale Metabolic Reconstructions for Industrial Applications

Cited by 5 publications

References 123 publications

Optimization of high quality total RNA isolation from the microalga, Chlorella sp. (Trebouxiophyceae, Chlorophyta) for next‐generation sequencing

Optimization of high quality total RNA isolation from the microalga, Chlorella sp. (Trebouxiophyceae, Chlorophyta) for next‐generation sequencing

Microalgas como ingrediente e suplemento dietético para tilápia <i>Oreochromis niloticus</i>: valor biológico, desempenho e composição da carcaça

Microalgae, a Biological Resource for the Future

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