Microanalysis of DNA by stripping transfer voltammetry

Jelen, František; Kouřilová, Alena; Pečínka, Petr; Paleček, Emil

doi:10.1016/j.bioelechem.2003.10.021

Cited by 40 publications

(54 citation statements)

References 11 publications

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“…The AdTSV technique has been primarily developed to analyse large biomacromolecules using their strong adsorption on the electrode surface [32,43]. Later we applied AdTSV also to low-molecular complexes between nucleic acid bases in connection with the mercury electrode [6,40]. Unlike AdSV, AdTSV can be compared with the medium exchange technique used in electrochemical measurements under microfluidic control where after the electrodeposition step the sample cell solution is replaced by a deaerated solution composed of an electrolyte [44].…”

Section: Electrochemical Behaviour Of Cu(i)-purinementioning

confidence: 99%

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

et al. 2010

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Electrochemical oxidations of aminopurines (adenine, 2-aminopurine, 2,6-diaminopurine) and their complexes with Cu(I) on a pencil graphite electrode were investigated by means of linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS). The anodic process of the Cu(I)-aminopurine complex, corresponding to the oxidation of Cu(I) to Cu(II), takes place in the potential range between 0.4 and 0.5 V (vs. Ag/AgCl/3 M KCl). At more positive potentials the aminopurines provide voltammetric peaks resulting from the oxidation of the purine ring. The stability of the accumulated complex layer was investigated by the adsorptive transfer stripping technique.

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Section: Electrochemical Behaviour Of Cu(i)-purinementioning

confidence: 99%

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

et al. 2010

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“…The Os,L complexes form stable covalent compounds with thymine and to a much less extent with cytosine; there is practically no reaction with adenine or guanine [10,12,14]. Pyrimidine residues in the B-form of dsDNA do not react with those complexes.…”

Section: Introductionmentioning

confidence: 97%

Voltammetric Behavior of Osmium-Labeled DNA at Mercury Meniscus-Modified Solid Amalgam Electrodes. Detecting DNA Hybridization

et al. 2006

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The complex of osmium tetroxide with 2,2'-bipyridine has been utilized as a probe of DNA structure and an electroactive marker of DNA in DNA hybridization sensors. It produces several voltammetric signals, the most negative of them has been observed only at mercury electrodes. This signal is of catalytic nature affording a high sensitivity of DNA determination. The catalytic current due to evolution of hydrogen in voltammetry of DNA modified by complex of osmium tetroxide with 2,2'-bipyridine (DNA-Os,bipy) was studied. Solid amalgam electrodes (modified with mercury menisci) of silver (m-AgSAE), copper (m-CuSAE), gold, and of combined bismuth and silver, were used as possible substitutes for mercury electrodes. Besides the hanging mercury drop electrode (HMDE), the catalytic current was observed only on m-AgSAE and m-CuSAE. Electrodes of gold and bismuth amalgams did not give the catalytic current. The detection limit of DNA-Os,bipy on HMDE was 0.1 ng mL À1 (RSD ¼ 2.3 %, N ¼ 11), and on m-AgSAE 0.2 ng mL À1 (RSD ¼ 3.1%, N ¼ 11). The m-AgSAE was successfully applied as a detection electrode in double-surface DNA hybridization experiments offering highly specific discrimination between complementary (target) and nonspecific DNAs, as well as determination of the length of a repetitive DNA sequence. The m-AgSAE has proved a convenient alternative to the HMDE or carbon electrodes used for similar purposes in previous work.

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“…Among these approaches, DNA osmium labeling appears to be most suitable for pyrimidine-rich DNAs because of the reactivity of Os, bipy towards (predominantly) thymine (while direct DNA voltammetry at carbon electrodes and CSV are better suited for purine-rich DNA strands). Moreover, as purines do not considerably react with Os, bipy [20,21], modification of DNAwith this complex does not abolish DNA hybridization via homopurine stretches. Target DNAs containing (A) n stretches can thus be osmium-labeled prior to capturing at DBT [25,27] (we have observed that the same was true when DNA was hybridized via other homopurine sequences [M. Fojta et al, unpublished]) (Fig.…”

Section: Dna Hybridization At Magnetic Beads and Dna Osmium Labelingmentioning

confidence: 99%

“…This feature makes Os, bipy and some other Os,L excellent DNA structural probes [20,23]. At the mercury or carbon electrodes, Os,L as well as Os,L-modified DNA (DNA-Os,L) undergo several (quasi)reversible faradaic processes corresponding to consecutive reduction/oxidation of osmium atom [19,21,22,24]. We have recently shown [22] that DNA-Os, bipy yields a specific peak (peak a) at the pyrolytic graphite electrode (PGE) whose potential significantly differs from that of a corresponding signal of free Os, bipy reagent.…”

Section: Introductionmentioning

confidence: 99%

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

et al. 2003

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Target DNAs, including a 71-mer oligonucleotide, a PCR product and a plasmid DNA, all containing oligo(A) stretches, were hybridized at magnetic Dynabeads oligo(dT) 25 (DBT). The hybridization events were detected using a technique based on chemical modification of the target DNA with a complex of osmium tetroxide with 2,2'-bipyridine (Os, bipy) and voltammetric detection at carbon electrodes. DNA was modified with Os, bipy prior to capture at DBT, at the beads, or after release from the beads. In the latter case, DNA-Os, bipy was detected in the reaction mixture using adsorptive transfer stripping voltammetry involving extraction of unreacted Os, bipy from the electrode by organic solvents. Pre-labeling of the target plasmid DNA and the PCR product with Os, bipy significantly increased the yield of DNA captured at the beads. Tens of femtomoles of both short (the 71-mer oligonucleotide) and long (the 3-kilobase plasmid) target DNAs in a 20-microliter hybridization sample can be easily detected by means of these techniques. Various carbon electrode materials, including pyrolytic graphite (PGE), highly oriented pyrolytic graphite (HOPGE), carbon paste (CPE), glassy carbon and pencil graphite, were tested regarding their suitability for the detection of osmium-labeled DNA. Among them, PGE and HOPGE appeared usable in the measurements of both purified DNA-Os, bipy and its mixtures with unreacted Os, bipy while CPE was suitable for the detection purified osmium-labeled DNA.

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Microanalysis of DNA by stripping transfer voltammetry

Cited by 40 publications

References 11 publications

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

Voltammetric Study of Aminopurines on Pencil Graphite Electrode in the Presence of Copper Ions

Voltammetric Behavior of Osmium-Labeled DNA at Mercury Meniscus-Modified Solid Amalgam Electrodes. Detecting DNA Hybridization

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

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