Microarchitectonic of Decellularized Bovine Pericardium Matrix

Abstract

The use of xenotissue for the needs of regenerative and cardiac medicine is a promising area of tissue engineering. The decellularization process provides complete purification of the elastin-collagen matrix of the bovine pericardium from cells and their components. The use of high concentrations of sodium dodecyl sulfate and glutaraldehyde can lead to a damage of the matrix architectonics. Therefore, the purpose of this study was to study the microarchitectonics of the decellularized matrix using a low concentration of sodium dodecyl sulfate (0.1% solution) and avoiding glutaraldehyde. Further stabilization and fixation of the matrix was carried out using 10 mM 1-Ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride and 10 mM N-Hydroxysuccinimide. The effect of decellularization was assessed by staining the samples with hematoxylin-eosin and by scanning electron microscopy. The research results confirmed the absence of structural changes in the collagen-elastin fibers of the matrix. Thus, the efficiency of using the proposed technology for obtaining a decellularized matrix based on the bovine pericardium was confirmed.