Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation

Chen, Sanyang; Fang, Hongbo; Li, Jie; Shi, Jihua; Zhang, Jiakai; Wang, Zhihui; Han, Yang; Cao, Shengli; Zhang, Huapeng; Tang, Hongwei; Guo, Wenzhi; Zhang, Shuijun

doi:10.1155/2019/5604843

Cited by 15 publications

(16 citation statements)

References 59 publications

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“…MiR-135a-5p may also act as a tumor suppressor in the development of colorectal adenomas and promote apoptosis of colon cancer cells ( 38 ). Here, we demonstrated that miR-135a-5p also targeted ATF3 ( 39 ). Our results also revealed the importance of miR-135a-5p in BTZ drug sensitivity in MM.…”

Section: Discussionmentioning

confidence: 77%

Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis

et al. 2021

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Multiple myeloma (MM) is a malignant cancer with an increasing in incidence that can be alleviated through bortezomib (BTZ) treatment. Activating transcription factor 3 (ATF3) plays a major role in cancer development. Moreover, microRNAs (miRNAs) regulate carcinogenic pathways, apoptosis, and programmed necrotic cell death. However, the detailed mechanism by which ATF3 modulates BTZ drug sensitivity/resistance remains elusive. In the current study, expression of ATF3 was significantly increased under BTZ treatment in a dose-dependent manner in MM cell lines. In addition, ATF3 could regulate cell apoptosis under BTZ treatment. The effect of ATF3 was negatively regulated by its binding miRNA, miR-135a-5p. When either ATF3 was silenced or miR-135a-5p mimics were added to MM cells, they partially lost sensitivity to BTZ treatment. This was accompanied by low levels of Noxa, CHOP, and DR5, and a decrease in mitochondrial membrane potential. These results revealed the combinatorial regulatory patterns of ATF3 and miR-135a-5p in the regulatory protein interactome, which indicated a clinical significance of the miR-135a-5p-ATF3 protein interaction network in BTZ therapy. This study provides potential evidence for further investigation into BTZ resistance.

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Section: Discussionmentioning

confidence: 77%

Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis

et al. 2021

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“…The mRNA levels of NLRP3, caspase-1, caspase-11, IL-1β, and IL-18 were measured by RT-qPCR. Total RNA was extracted from tissues using the TRIzol (Thermo Fisher Scientific, Shanghai, China), and reverse transcription was performed as described previously (26). Primers were designed based on the gene sequences acquired from PubMed.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

“…The primers were synthesized by Invitrogen (Shanghai) Trading Co., Ltd., China, and are shown in Table 1. The primers were diluted appropriately, PCR amplification was performed, and RT-qPCR results were analyzed using a relative quantitative method as described previously (26).…”

Section: Reverse Transcription-quantitative Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

Caspase-1 Inhibitor Reduces Pyroptosis Induced by Brain Death in Kidney

et al. 2021

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Brain death (BD) induces an organ-level inflammatory response. However, the underlying mechanisms have not been fully elucidated. Here, we investigated the role of caspase-1-mediated pyroptosis in BD-induced kidney injury in rats. A BD model was established in Sprague-Dawley rats. The rats were intravenously injected with Z-YVAD-FMK 1 h before BD, and sham-operated rats served as controls. After 0, 1, 2, 4, and 6 h of BD, renal injury, and renal expression of the nod-like receptor family pyrin domain-containing 3 (NLRP3), caspase-1, caspase-11, gasdermin D (GSDMD), IL-1β, and IL-18 were assessed using quantitative reverse transcriptase-polymerase chain reaction, western blotting, and immunohistochemistry. Blood urea nitrogen and serum creatinine levels were measured. Additionally, renal tubular epithelial cells (NRK-52E) were subjected to 3 h of hypoxia followed by 6 h of reoxygenation and incubated with Z-YVAD-FMK before hypoxia and reoxygenation. Caspase-11 was knocked-down using small interfering RNA technology. Cell viability and levels of pyroptosis-associated proteins were assessed thereafter. NLRP3, caspase-1, GSDMD, IL-1β, and IL-18 expression levels were upregulated in BD rats. Treatment with Z-YVAD-FMK reduced mRNA and protein levels of caspase-1, GSDMD, IL-1β, and IL-18, improved renal function, and alleviated renal injury. Z-YVAD-FMK efficaciously reduced pyroptosis effects in kidneys in BD rats. Thus, it could be considered as a therapeutic target for BD-induced kidney injury.

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“…The mRNA levels of NLRP3, caspase-1, caspase-11, IL-1β, and IL-18 were measured by RT-qPCR. Total RNA was extracted from tissues using the TRIzol (Thermo Fisher Scienti c, Shanghai, China), and reverse transcription was performed as described previously [20]. Primers were designed based on the gene sequences acquired from PubMed.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

“…The primers were synthesized by Invitrogen (Shanghai) Trading Co., Ltd., China, and are shown in Table S1. The primers were diluted appropriately, PCR ampli cation was performed, and RT-qPCR results were analyzed using a relative quantitative method as described previously [20].…”

Section: Reverse Transcription-quantitative Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

Caspase-1 Inhibitor Reduces Pyroptosis in Kidney Injury Induced by Brain Death

Liu¹,

Yang²,

Shi³

et al. 2021

Preprint

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Brain death (BD) induces an organ-level inflammatory response, and BD donor kidneys show inferior survival and recovery rates. However, the underlying mechanisms have not been fully elucidated. Here, we investigated the role of caspase-1-mediated pyroptosis in BD-induced kidney injury in rats. A BD model was established in Sprague-Dawley rats. The rats were intravenously injected with a caspase-1 inhibitor (Z-YVAD-FMK) 1 h before BD, and sham-operated rats served as controls. After 0, 1, 2, 4, and 6 h of BD, renal function, renal injury, and renal expression of NLRP3, caspase-1, caspase-11, gasdermin D (GSDMD), IL-1β, and IL-18 were assessed using quantitative reverse transcriptase-polymerase chain reaction, western blotting, and immunohistochemistry. Blood urea nitrogen and serum creatinine levels were measured. Additionally, renal tubular epithelial cells (NRK-52E) were subjected to 3 h of hypoxia followed by 6 h of reoxygenation and incubated with Z-YVAD-FMK before hypoxia and reoxygenation. Caspase-11 was knocked-down using small interfering RNA technology. Cell viability and levels of pyroptosis-associated proteins were assessed thereafter. NLRP3, caspase-1, GSDMD, IL-1β, and IL-18 expression levels were upregulated in BD rats. Treatment with Z-YVAD-FMK reduced mRNA and protein levels, improved renal function, and alleviated renal injury. Z-YVAD-FMK effectively reduced pyroptosis in BD rats; however, it did not affect caspase-11 expression in vivo or in vitro. Thus, it could be considered as a therapeutic target for BD-induced kidney injury.

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Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation

Cited by 15 publications

References 59 publications

Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis

Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis

Caspase-1 Inhibitor Reduces Pyroptosis Induced by Brain Death in Kidney

Caspase-1 Inhibitor Reduces Pyroptosis in Kidney Injury Induced by Brain Death

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