Microarray analysis of differentially expressed genes in mouse bone marrow tissues after ionizing radiation

Abstract

Ionizing radiation (IR) affects the expression of a series of genes including genes involved in G1/S transition and the P53 pathway. Among those, reduction of SIRT1 was seen to be involved in transactivation of P53.

Identification of Hub Genes Related to the Recovery Phase of Irradiation Injury by Microarray and Integrated Gene Network Analysis

“…Therefore, we can conclude that the responses of irradiated bone marrow tissue are different at the stage of injury compared to recovery. For example, p53 and its downstream target genes (such as, cyclin G1 and Bax ) which induce cell-cycle arrest, DNA repair and apoptosis are up-regulated after IR [8], [32], but we observed no expression change of these genes during the recovery phase in our experiment.…”

“…Microarray analysis of irradiated mice at the recovery phase has not been report so far. Dai et al reported that in murine BM 6 h after 6.5 Gy gamma irradiation, the functional categories of differentially expressed genes are comprised of biological processes including DNA replication/repair, proliferation/apoptosis, cell cycle control and RNA processing [8]. Therefore, we can conclude that the responses of irradiated bone marrow tissue are different at the stage of injury compared to recovery.…”

“…At 3, 7, 11 and 21 days after irradiation (DAI) or 0 day (sham-irradiation), 0.5–1 ml of blood was collected and the animals were euthanized. Whole bone marrow cells from both tibias were collected using a previously published protocol [8], [46]. Six to eight mice were used for each time point to collect enough cells for RNA extraction.…”

“…identified 34 up-regulated and 69 down-regulated genes in murine bone marrow 6 h after 6.5 Gy gamma radiation when compared to the expression levels in untreated bone marrow. These genes participate in DNA replication/repair, proliferation/apoptosis, cell cycle control and RNA processing [8]. Previous groups have used microarray to determine the effect of acute high-dose or prolonged low-dose IR exposure on gene expression in various tissues, including: kidneys, testes and brain [9]–[13].…”

Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC−/−) mice

“…However, upon immune activation, Th polarization is shifted towards the Th 1 -type reactions and this evolves into a more effective immune response. Since the first days after irradiation are especially characterized by inflammatory processes [27], this altered immune milieu in HDC -/-mice could explain the more protective reactions observed during the early phase of extramedullary hematopoiesis.…”