Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens

Kano, Rika; Konnai, Satoru; Onuma, Misao; Ohashi, Kazuhiko

doi:10.1292/jvms.71.603

Cited by 19 publications

(17 citation statements)

References 40 publications

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“…The CD8α gene was significantly up-regulated at 10dpi in the MD-resistant chicken line (line 6 3 ) and RCS-M, but down-regulated in the MD-susceptible chicken line (line 7 2 ). In chickens vaccinated against MDV an increase of CD8α cells was found after MDV infection compared to unvaccinated chickens [32]. The vaccinated birds were phenotypically similar to line 6 3 and hence, this result is consistent with our finding.…”

Section: Discussionsupporting

confidence: 91%

Temporal transcriptome changes induced by MDV in marek's disease-resistant and -susceptible inbred chickens

Luo

Mitra

et al. 2011

BMC Genomics

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BackgroundMarek's disease (MD) is a lymphoproliferative disease in chickens caused by Marek's disease virus (MDV) and characterized by T cell lymphoma and infiltration of lymphoid cells into various organs such as liver, spleen, peripheral nerves and muscle. Resistance to MD and disease risk have long been thought to be influenced both by genetic and environmental factors, the combination of which contributes to the observed outcome in an individual. We hypothesize that after MDV infection, genes related to MD-resistance or -susceptibility may exhibit different trends in transcriptional activity in chicken lines having a varying degree of resistance to MD.ResultsIn order to study the mechanisms of resistance and susceptibility to MD, we performed genome-wide temporal expression analysis in spleen tissues from MD-resistant line 63, susceptible line 72 and recombinant congenic strain M (RCS-M) that has a phenotype intermediate between lines 63 and 72 after MDV infection. Three time points of the MDV life cycle in chicken were selected for study: 5 days post infection (dpi), 10dpi and 21dpi, representing the early cytolytic, latent and late cytolytic stages, respectively. We observed similar gene expression profiles at the three time points in line 63 and RCS-M chickens that are both different from line 72. Pathway analysis using Ingenuity Pathway Analysis (IPA) showed that MDV can broadly influence the chickens irrespective of whether they are resistant or susceptible to MD. However, some pathways like cardiac arrhythmia and cardiovascular disease were found to be affected only in line 72; while some networks related to cell-mediated immune response and antigen presentation were enriched only in line 63 and RCS-M. We identified 78 and 30 candidate genes associated with MD resistance, at 10 and 21dpi respectively, by considering genes having the same trend of expression change after MDV infection in lines 63 and RCS-M. On the other hand, by considering genes with the same trend of expression change after MDV infection in lines 72 and RCS-M, we identified 78 and 43 genes at 10 and 21dpi, respectively, which may be associated with MD-susceptibility.ConclusionsBy testing temporal transcriptome changes using three representative chicken lines with different resistance to MD, we identified 108 candidate genes for MD-resistance and 121 candidate genes for MD-susceptibility over the three time points. Genes included in our resistance or susceptibility genes lists that are also involved in more than 5 biofunctions, such as CD8α, IL8, USP18, and CTLA4, are considered to be important genes involved in MD-resistance or -susceptibility. We were also able to identify several biofunctions related with immune response that we believe play an important role in MD-resistance.

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Section: Discussionsupporting

confidence: 91%

Temporal transcriptome changes induced by MDV in marek's disease-resistant and -susceptible inbred chickens

Luo

Mitra

et al. 2011

BMC Genomics

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“…Many MDV-induced downregulated genes are critical for an effective antiviral immune response. Kano et al [13] found that the expression of T-cell receptor 1-related genes was upregulated in vaccinated-challenged chickens compared to unvaccinated chickens during the latent phase of infection. However, a comprehensive gene expression in response to MDV infection in the thymuses of chickens at different stages of viral pathogenesis - including the cytolytic, latent, and transformation phases - remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Analysis of Host Responses to Marek's Disease Virus Infection in Chicken Thymus

Qin²,

et al. 2015

Intervirology

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Marek's disease virus (MDV) is a cell-associated alpha-herpesvirus that causes T-cell lymphomas and nervous disorders in chickens. Different from other lymphoid organs, the thymus is the site of T-cell maturation and differentiation. However, the transcriptional response to MDV infection in the chicken thymus is still not known. In this study, we performed genome-wide expression analysis in thymus tissues of RB1B-infected chickens at different time points to investigate the molecular mechanisms of MDV pathogenesis. The number of differentially expressed genes with 2-fold or higher changes (>2) are as follows: 1,250 genes (7 dpi), 834 genes (14 dpi), 1,958 genes (21 dpi), and 2,306 genes (28 dpi). Gene ontology enrichment analysis revealed that the upregulated genes were involved in immune and inflammatory response at 7 dpi; angiogenesis, cytoskeleton organization, cell adhesion, and signal transduction showed different expressions at 21 and 28 dpi. The expression pattern of 18 randomly selected genes was confirmed by real-time RT-PCR. Several differently expressed host genes associated with tumor development are discussed. We identified the global host-gene expression pattern in the thymus of chickens that responded to MDV infection. The present data may provide groundwork for future investigation in the biology and pathogenesis of MDV.

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“…Several studies have been performed to identify the gene expression profile in in-vivo and in-vitro MDV infections using microarray analysis (Yonash et al, 1999;Morgan et al, 2001;Sarson et al, 2006;Kano et al, 2009;Heidari et al, 2010;Smith et al, 2011;Lian et al, 2012, Subramaniam et al, 2013.. The molecular mechanism of the host response to GX0101∆LTR MDV was elucidated in the current study.…”

Section: Discussionmentioning

confidence: 95%

Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Marek’s Disease Virus of GX0101 ∆ LTR

Cui

et al. 2017

Rev. Bras. Cienc. Avic.

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Marek's disease (MD), a lymphoproliferative disorder of chickens caused by the MD virus (MDV), is economically significant. The resistance/susceptibility to MD is controlled by host genetics. The host response to different virus strains varies. The pathogenicity of REV-LTR deleted GX0101∆LTR MDV has been previously reported. However, the precise molecular mechanism of the response of chickens to GX0101∆LTR remains unclear. The current study aimed at identifying the genes and pathways involved in the response to GX0101∆LTR virus infection in specific pathogen-free chicken embryo fibroblast cells using global transcriptome analysis. A total of 1,633 genes associated with GX0101∆LTR infection were identified. Functional analysis showed that the cytokine-cytokine receptor interaction plays an important role in the response to GX0101∆LTR infection.

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Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens

Cited by 19 publications

References 40 publications

Temporal transcriptome changes induced by MDV in marek's disease-resistant and -susceptible inbred chickens

Temporal transcriptome changes induced by MDV in marek's disease-resistant and -susceptible inbred chickens

Transcriptional Analysis of Host Responses to Marek's Disease Virus Infection in Chicken Thymus

Transcriptome Analysis of Chicken Embryo Fibroblast Cell Infected with Marek’s Disease Virus of GX0101 ∆ LTR

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