Microarray Analysis of LIF/Stat3 Transcriptional Targets in Embryonic Stem Cells

Sekkaı̈, Dalila; Gruel, Gaëtan; Herry, Magali; Moucadel, Virginie; Constantinescu, Stefan N.; Albagli, Olivier; Roux, Diana Tronik‐Le; Vainchenker, William; Bennaceur‐Griscelli, Annelise

doi:10.1634/stemcells.2005-0182

Cited by 62 publications

(47 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2, 3A). These results are consistent with DNA microarray data of others who reported Brf1 (Zfp36L1) downregulation after LIF removal [41,42]. That the effect in some clones was less intense than that following LIF removal suggests that not all effects on gene expression by LIF removal are mediated by Stat3.…”

Section: Discussionsupporting

confidence: 92%

A Cassette System to Study Embryonic Stem Cell Differentiation by Inducible RNA Interference

et al. 2007

View full text Add to dashboard Cite

Although differentiation of pluripotent embryonic stem cells is restricted by a hierarchy of transcription factors, little is known about whether post-transcriptional mechanisms similarly regulate early embryoid differentiation. We developed a system where small hairpin (sh)RNAs can be induced in embryonic stem (ES) cells from a defined locus following integration by Flp recombinase-mediated DNA recombination. To verify the system, the key transcription factor Stat3, which maintains pluripotency, was downregulated by shRNA, and the expected morphological and biochemical markers of differentiation were observed. Induction of shRNA specific for the post-transcriptional regulator Brf1 (Zfp36L1) amplified the cardiac markers with strong stimulation of cardiomyocyte formation within embryoid bodies. These findings identify Brf1 as a novel potential regulator of cardiomyocyte formation and suggest that post-transcriptional mechanisms are of importance to early development and, possibly, to regenerative medicine. The inducible RNA interference system presented here should also allow assignment of function for candidate genes with suspected roles in ES cell development.

show abstract

Section: Discussionsupporting

confidence: 92%

A Cassette System to Study Embryonic Stem Cell Differentiation by Inducible RNA Interference

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Recently, microarray analysis was carried out to isolate the LIF/STAT3 transcriptional targets in mES cells, and Aes (amino-terminal enhancer of split) gene, one of the targets, was found to be downregulated by LIF removal (Sekkaï et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Identification of Jmjd1a as a STAT3 Downstream Gene in mES Cells

Kang

Lee

et al. 2006

Cell Struct. Funct.

View full text Add to dashboard Cite

ABSTRACT. Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. To look for STAT3-downstream target genes, we performed DD-RT PCR in the presence or absence of LIF. Through further confirmation, we finally selected 8 genes whose expressions were significantly dependent upon the presence of LIF. Among them, Jmjd1a was down-regulated after LIF withdrawal, and it was selected for further investigation. Its expression started to decrease 1 day after the removal of LIF, and disappeared on day 3. It was also shown that STAT3 could bind to the promoter region of Jmjd1a gene. These data demonstrate that Jmjd1a might be a critical signaling molecule underlying the maintenance of pluripotency in mES cells.

show abstract

“…They may also predict and/or analyze activation events in relation to others, and may provide temporal delineation of these events. Predictions of interactions, and perhaps outcomes, of complex signaling cascades have been modeled in response to various stimuli (22), and the use of microarray-based technologies have yielded astonishing amounts of data describing transcription factor complexes and genes which are induced as a result of Stat3 activation in specific cellular systems, including stem cells (23,24). The perturbation of levels of Stat3 protein in one study related to the placenta permits the description of pathways which are potentially activated in the presence and absence of Stat3, and indicates that several transcription factors coordinately regulate groups of genes which potentially determine functional output (25).…”

Section: Cellular Contextmentioning

confidence: 99%

STATus and Context within the Mammalian Nervous System

Rajan

2011

Mol Med

View full text Add to dashboard Cite

Effective manipulation of human disease processes may be achieved by understanding transcriptional, posttranscriptional and epigenetic events that orchestrate cellular events. The levels of activation of specific molecules, spatial distribution and concentrations of relevant networks of signaling molecules along with the receptiveness of the chromatin to these signals are some of the parameters which dictate context. Effects elicited by the transcription factor signal transducers and activator of transcription 3 (Stat3) are discussed with respect to the context within which Stat3-mediated effects are elicited within the developing and adult mammalian nervous system. Stat3 signals are pivotal to the proliferation and differentiation of neural stem cells. They also participate in neuronal regeneration and cancers of the nervous system. An analysis of the context in which Stat3 activation occurs in these processes provides a potential predictive paradigm with which novel methods for intervention may be designed.

show abstract

Microarray Analysis of LIF/Stat3 Transcriptional Targets in Embryonic Stem Cells

Cited by 62 publications

References 49 publications

A Cassette System to Study Embryonic Stem Cell Differentiation by Inducible RNA Interference

A Cassette System to Study Embryonic Stem Cell Differentiation by Inducible RNA Interference

Identification of Jmjd1a as a STAT3 Downstream Gene in mES Cells

STATus and Context within the Mammalian Nervous System

Contact Info

Product

Resources

About