Microarray Analysis of Normal and Abnormal Chick Ventricular Myocardial Development

Abstract

The left and right ventricle originate from distinct parts of the cardiac tube, and several genes are known to be differentially expressed in these compartments. The aims of this study were to determine developmental differences in gene expression between the left and right ventricle, and to assess the effect of altered hemodynamic loading. RNA was extracted from isolated left and right normal chick embryonic ventricles at embryonic day 6, 8, and 10, and from day 8 left atrial ligated hearts with hypoplastic left and dilated right ventricles. cRNA was hybridized to Affymetrix Chicken Genome array according to manufacturer protocols. Microarray analysis identified 302 transcripts that were differentially expressed between the left and right ventricle. Comparative analysis detected 91 genes that were different in left ventricles of ligated hearts compared to age-matched ventricles, while 66 were different in the right ones. A large number of the changes could be interpreted as a delay of normal maturation. The approach described in this study could be used as one of the measures to gauge success of surgical procedures for congenital heart disease and help in determining the optimal time frame for intervention to prevent onset of irreversible changes.

Mechanosensitive Pathways in Heart Development: Findings from Chick Embryo Studies

“…Since LAL is a side specific surgery mainly affecting the left side of the heart, it was reported that it alters hemodynamics in a side-specific manner [25]. Krejčí et al conducted a study which unraveled differential gene expression between control and LAL hearts at ED8 [45]. The microarray analyses identified 91 genes that were differently expressed between control and LAL hearts, while only 66 were different between right ventricles and left ventricles.…”

Myocardial Biomechanics and the Consequent Differentially Expressed Genes of the Left Atrial Ligation Chick Embryonic Model of Hypoplastic Left Heart Syndrome

“…A previous study have also attempted to characterize the gene expressions in the LV after LAL through microarray analysis, to assist in pinpointing the mechanobiological pathways that may be causing LV hypoplasia [ 22 ]. A total of 91 differentially express genes in the LV and 66 gene in the right ventricle were reported.…”