Microarray analysis reveals the involvement of beta-2 microglobulin (B2M) in human osteoarthritis

Zhang, H.; Liew, Choong Chin; Marshall, K. W.

doi:10.1053/joca.2002.0850

Cited by 51 publications

(39 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Results from a recent study indicated that B2M was highly expressed in OA cartilage compared to normal and it may have a role in OA pathogenesis [20]. In this study, although B2M levels in OA were significantly higher those found in normal synovial fluid, there was no significant difference in B2M levels between the different OA stages according to a method grading OA severity at arthroscopy.…”

Section: Discussioncontrasting

confidence: 60%

Relationship between radiographic grading of osteoarthritis and serum beta-2 microglobulin

2008

View full text Add to dashboard Cite

show abstract

Section: Discussioncontrasting

confidence: 60%

Relationship between radiographic grading of osteoarthritis and serum beta-2 microglobulin

2008

View full text Add to dashboard Cite

show abstract

“…Taken together these results indicate that MMP2 and PCOLCE levels are connected to each other possibly due to a protective feedback loop. B2M has previously been proposed to be involved in OA pathogenesis as B2M is expressed in OA cartilage and may influence chondrocyte gene expression as well as inhibit proliferation of chondrocytes [39]. The medially up-regulated B2M levels found in the LM scored individual could potentially balance the proliferative stress signals that may be initiated by normal joint usage and/or be the early response to a low grade OA and thus a potential early marker of OA.…”

Section: Discussionmentioning

confidence: 99%

“…FSTL1 has been demonstrated to be correlated to the severity of OA disease as well as CHI3L1 [43-45]. SPARC and B2M are correlated to OA while the IGFBP7 has not been correlated to OA previously [30,39,46]. IGFBP4 is suggested to inhibit the canonical Wnt signaling pathway, which makes elevated levels of IGFBP4 on the medial side in the LM scored specimen an interesting finding as stimulated Wnt signaling is known to be involved in OA pathogenesis [47-50].…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients

et al. 2013

View full text Add to dashboard Cite

BackgroundOsteoarthritis (OA) is a destructive joint disease and there are no known biomarkers available for an early diagnosis. To identify potential disease biomarkers and gain further insight into the disease mechanisms of OA we applied quantitative proteomics with SILAC technology on the secretomes from chondrocytes of OA knees, designated as high Mankin (HM) scored secretome. A quantitative comparison was made between the secretomes of the medial and lateral femur condyle chondrocytes in the same knee since the medial femur condyle is usually more affected in OA than the lateral condyle, which was confirmed by Mankin scoring. The medial/lateral comparison was also made on the secretomes from chondrocytes taken from one individual with no clinically apparent joint-disease, designated as low Mankin (LM) scored secretome.ResultsWe identified 825 proteins in the HM secretome and 69 of these showed differential expression when comparing the medial and lateral femoral compartment. The LM scored femoral condyle showed early signs of OA in the medial compartment as assessed by Mankin score. We here report the identification and relative quantification of several proteins of interest for the OA disease mechanism e.g. CYTL1, DMD and STAB1 together with putative early disease markers e.g. TIMP1, PPP2CA and B2M.ConclusionsThe present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms.

show abstract

“…27 DNA microarray has revealed 20 genes that are regulated by beta-2 microglobulin in osteoarthritic chondrocytes. 28 Lorenz et al 29 used a combinatorial analysis of proteome and transcriptome to compare rheumatoid arthritis and OA patients, and characterized each pathology by evaluation of each approach.…”

Section: O S T E O a R T H R I T I S P A T H O L O G Ymentioning

confidence: 99%

Molecular network of cartilage homeostasis and osteoarthritis

Hashimoto

Nakasa

Hikata

et al. 2007

Medicinal Research Reviews

104

View full text Add to dashboard Cite

This review article presents the current understanding of the molecular basis of articular cartilaginous homeostasis, and outlines potential areas to focus on within the developing field of therapeutics for cartilage disorders. Articular cartilage, an integral component of joints in extremities and the vertebral column, is essential for locomotion. Disturbance of joint development or cartilage homeostasis causes congenital osteocartilaginous dysplasia or osteoarthritic diseases, respectively. Symptomatic treatments and surgical replacement of joints are effective but can also be problematic in terms of quality of life over time. Recently, new insights into the molecular biological basis of chondrocyte differentiation and cartilage homeostasis have been reported. While joint formation is regulated by several growth factors such as Wnts (wingless-related MMTV integration site) and Gdfs (growth and differentiation factors), the pathology of osteoarthritis is now interpreted as the disruption of balance between anabolic and catabolic signals. Current findings in molecular biology on joint development are reviewed concisely to aid in the understanding of the molecular network that governs articular cartilage development and homeostasis.

show abstract

Microarray analysis reveals the involvement of beta-2 microglobulin (B2M) in human osteoarthritis

Abstract: These results indicate that B2M is highly expressed in OA cartilage and synovial fluid compared to normal, and suggest that B2M may have effects on chondrocyte function that could contribute to OA pathogenesis. Published by Elsevier Science Ltd.

Cited by 51 publications

References 35 publications

Relationship between radiographic grading of osteoarthritis and serum beta-2 microglobulin

Relationship between radiographic grading of osteoarthritis and serum beta-2 microglobulin

Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients

Molecular network of cartilage homeostasis and osteoarthritis

Contact Info

Product

Resources

About