2014
DOI: 10.3390/ijms15045907
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Microarray-Based Gene Expression Profiling to Elucidate Effectiveness of Fermented Codonopsis lanceolata in Mice

Abstract: In this study, the effect of Codonopsis lanceolata fermented by lactic acid on controlling gene expression levels related to obesity was observed in an oligonucleotide chip microarray. Among 8170 genes, 393 genes were up regulated and 760 genes were down regulated in feeding the fermented C. lanceolata (FCL). Another 374 genes were up regulated and 527 genes down regulated without feeding the sample. The genes were not affected by the FCL sample. It was interesting that among those genes, Chytochrome P450, Dmb… Show more

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Cited by 3 publications
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“…All these commonly used methods have shown shortcomings, such as Northern blot being an experience-requiring and time-consuming technique [ 15 ], while the selection of appropriate internal standard RNAs significantly affects the accuracy of competitive RT-PCR experiments [ 16 ]. To ensure the veracity and repeatability of experiments, high-throughput methods, such as microarrays or RNA-seq, are always expensive and not only require high quality, but also a significant amount of RNA samples [ 17 ]. Compared to these methods, qRT-PCR has many advantages, such as high sensitivity, high specificity, high accuracy, ease of operation, and lower consumption of supplies.…”
Section: Introductionmentioning
confidence: 99%
“…All these commonly used methods have shown shortcomings, such as Northern blot being an experience-requiring and time-consuming technique [ 15 ], while the selection of appropriate internal standard RNAs significantly affects the accuracy of competitive RT-PCR experiments [ 16 ]. To ensure the veracity and repeatability of experiments, high-throughput methods, such as microarrays or RNA-seq, are always expensive and not only require high quality, but also a significant amount of RNA samples [ 17 ]. Compared to these methods, qRT-PCR has many advantages, such as high sensitivity, high specificity, high accuracy, ease of operation, and lower consumption of supplies.…”
Section: Introductionmentioning
confidence: 99%
“…Currently, the assessment of gene expression patterns in plants is commonly accomplished using techniques such as Northern blotting, competitive reverse transcription-polymerase chain reaction (RT-PCR), microarray analysis, or quantitative reverse transcription-polymerase chain reaction (qRT-PCR) 26 . But, these techniques have their own liitations 28,29,30 . Instead, qRT-PCR offers numerous advantages, including heightened sensitivity, enhanced specificity, remarkable accuracy, userfriendly operation and reduced consumption of resources 31,32 .…”
mentioning
confidence: 99%