Microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes

Banér, Johan; Gyarmati, Péter; Yacoub, Alia; Hakhverdyan, Mikhayil; Stenberg, Johan A.; Ericsson, Olle; Nilsson, Mats; Landegren, Ulf; Belák, Sándór

doi:10.1016/j.jviromet.2007.03.004

Cited by 41 publications

(38 citation statements)

References 21 publications

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“…The other reagents involved in the reaction are standard molecular assay chemicals. Although the equipment used is currently uncommon in many diagnostic laboratories and the lack of automation of the assay is a certain limitation in this area, similar approaches are becoming widely accepted (5,7,8,16,22,24).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes

et al. 2008

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A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes

et al. 2008

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“…Upon the hybridization to target DNA, the probes become circularized by enzymatic ligation, while if there is no target DNA present, the padlock probes remain linear. Padlock probes have previously been used for genotyping (9,13), gene copy number (23), expression analysis (14), target sequencing (5), and mRNA splicing (3), as well as for detection of bacteria and other infectious pathogens (1,8,15). Only the circularized padlock probes are then amplified by the exponential rolling-circle amplification (RCA)-based C2CA reaction (2,4).…”

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Colorimetric Nucleic Acid Testing Assay for RNA Virus Detection Based on Circle-to-Circle Amplification of Padlock Probes

Zorzet

Göransson

et al. 2011

J Clin Microbiol

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We developed a molecular diagnostic method for detection of RNA virus based on padlock probes and colorimetric readout. The feasibility of our approach was demonstrated by using detection of Crimean-Congo hemorrhagic fever (CCHF) virus as a model. Compared with conventional PCR-based methods, our approach does not require advanced equipment, involves easier assay design, and has a sensitivity of 10 3 viral copies/ml. By using a cocktail of padlock probes, synthetic templates representing different viral strain variants could be detected. We analyzed 34 CCHF patient samples, and all patients were correctly diagnosed when the results were compared to those of the current real-time PCR method. This is the first time that highly specific padlock probes have been applied to detection of a highly variable target sequence typical of RNA viruses.

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“…?show "fnote_aff1"$^! "content-markup(./author-grp [1]/aff|./author-grp [1]/dept-list)> Vesicular stomatitis (VS) is an endemic disease in Mexico, Central America, and northern South America with periodic outbreaks observed in the southwestern United States. 7,10,[13][14][15]20 Although Vesicular stomatitis virus (VSV; order Mononegavirales, family Rhabdoviridae, genus Vesiculovirus) can infect horses, clinical signs in cattle and swine mimic that of foot-and-mouth disease (FMD) and include vesicular lesions and ulcerations of the tongue, mouth, and coronary bands of infected animals.…”

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“…To determine the exact nature of the problem, all From the National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada (Hole, Clavijo), and the Comisió n México-Estados Unidos para la Prevenció n de la Fiebre Aftosa y otras Enfermedades Exoticas de los Animales, Cuajimalpa, Mexico (Velazques-Salinas). 1 Corresponding Author: Alfonso Clavijo, National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg MB R3E 3M4, Canada. aclavijo@tvmdl.tamu.edu the strains were tested in the original real-time RT-PCR assay described previously 5 and assessed both by the realtime assay (Table 1) and by agarose gel electrophoresis of the real-time RT-PCR product.…”

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confidence: 99%

Improvement and Optimization of a Multiplex Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Detection and Typing of Vesicular Stomatitis Virus

2010

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Abstract. An improvement to a previously reported real-time reverse transcription polymerase chain reaction (real-time RT-PCR) assay for the detection of Vesicular stomatitis virus (VSV) is described. Results indicate that the new assay is capable of detecting a panel of genetically representative strains of VSV present in North, Central, and South America. The assay is specific for VSV and allows for simultaneous differentiation between Vesicular stomatitis Indiana virus and Vesicular stomatitis New Jersey virus. This realtime RT-PCR is able to detect current circulating strains of VSV and can be used for rapid diagnosis of VSV and differentiation of VSV from other vesicular diseases, such as foot-and-mouth disease.

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Microarray-based molecular detection of foot-and-mouth disease, vesicular stomatitis and swine vesicular disease viruses, using padlock probes

Cited by 41 publications

References 21 publications

Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes

Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes

Colorimetric Nucleic Acid Testing Assay for RNA Virus Detection Based on Circle-to-Circle Amplification of Padlock Probes

Improvement and Optimization of a Multiplex Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Detection and Typing of Vesicular Stomatitis Virus

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