Microarray-based screening of the microRNAs associated with caryopsis development in Oryza sativa

Liu, H.; Shen, Deyan; Jia, Shenghua; Li, W.; Li, J.; Liu, J.; Han, Shengcheng; Wang, Y.

doi:10.1007/s10535-012-0270-4

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…According to previous studies, miR164 abundance differs among various rice genotypes and among seeds at different developmental stages. For example, microarray analysis identified 27 miRNAs, excluding miR164 , in developing caryopses of the japonica cultivar Nipponbare at 5 and 12 d after heading (Liu et al ). In developing seeds of rice Baifeng B (an indica landrace), 21 conserved and 91 nonconserved miRNA families were identified using customized miRNA chips; moreover, miR164 transcripts increased rapidly from 2 to 6,871 reads from the soft‐dough stage (3–17 d after fertilization) to the hard‐dough stage (18–20 d after fertilization) (Lan et al ).…”

Section: Discussionmentioning

confidence: 99%

miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa‐miR164c was transcriptionally upregulated, while osa‐miR168a was downregulated in artificially aged seeds; this was verified by quantitative real‐time PCR analysis. Under the same aging condition, osa‐miR164c overexpression in OE164c transgenic seeds and osa‐miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa‐miR164c silencing in MIM164c and osa‐miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild‐type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa‐miR164c target genes (OsPM27 and OsPSK5) and osa‐miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa‐miR164c and osa‐miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.

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Section: Discussionmentioning

confidence: 99%

miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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“…In plants, miRNAs control a variety of biological processes, such as development and responses to phytohormones and stresses (Liu and Chen 2009, Sunkar et al 2012, Eldem et al 2013, Jin et al 2013, Liu et al 2013. In particular, a number of studies show that miRNAs are associated with a drought stress response (Zhou et al 2010, Khraiwesh et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava

Phookaew¹,

Netrphan²,

Sojikul³

et al. 2014

Biologia plant.

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Cassava (Manihot esculenta Crantz) is an important crop and it is significantly affected by water stress. The computational analysis of cis-regulatory elements in promoter regions of 21 drought-responsive miRNA gene families and 35 miRNA-target genes in cassava indicated some elements relevant to drought stress responses. To investigate the role of miRNAs and target genes in responses to a water deficit in cassava in more detail, in vitro plantlets were subjected to an imitated water deficit by 40 % polyethylene glycol. Using RT-qPCR, the differential expression of the cassava miR164/MesNAC and miR167/MesARF6/8 were observed to be associated with changes in the leaf shape, stomatal closure, and relative water content. The modified 5'-RNA ligase-mediated rapid amplification of cDNA-end (5'RLM-RACE) experiment confirmed MesNAC and MesARF8 as the in vivo-target genes of miR164 and miR167, respectively, in cassava leaf. The possible functions of miR164 and miR167-target genes in response to water deficit are discussed.

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Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants

2016

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MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs (sRNA) that play an essential role in the regulation of target mRNAs expressed during plant development and in response to stress. MicroRNA expression profiling has helped to identify miRNAs that regulate a range of processes, including the plant's defence response to pathogens. In this study, differential miRNA expression in own-rooted Vitis vinifera cv. Cabernet Sauvignon plants infected with grapevine leafroll-associated virus 3 was investigated with microarrays and next-generation sequencing (NGS) of sRNA and mRNA. These high-throughput approaches identified several differentially expressed miRNAs. Four miRNAs, identified by both approaches, were validated by stemloop RT-PCRs. Three of the predicted targets of the differentially expressed miRNAs were also differentially expressed in the transcriptome data of infected plants, and were validated by RT-qPCR. Identification of these miRNAs and their targets can lead to a better understanding of host-pathogen interactions involved in grapevine leafroll disease and the identification of possible targets for virus resistance.

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Microarray-based screening of the microRNAs associated with caryopsis development in Oryza sativa

Cited by 4 publications

References 45 publications

miR164c and miR168a regulate seed vigor in rice

miR164c and miR168a regulate seed vigor in rice

Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava

Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants

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