Microarray detection of fungal infection in pulmonary tuberculosis

Osman, Nehad M.; Gomaa, Ashraf A.; Sayed, Nehad; Aziz, Ashraf Abdel

doi:10.1016/j.ejcdt.2013.02.002

Cited by 21 publications

(21 citation statements)

References 20 publications

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“…A. fumigatus was the dominant Aspergillus species detected in CPA patients followed by A. niger and A. flavus . Previous studies from Africa and Asia demonstrated the same distribution of Aspergillus species among TB patients, with A. fumigatus most common but with significant presence of A. niger and A. flavus [ 4 , 23 , 24 , 25 ]. This is different from the culture result of CPA patients from the UK, which was dominated by A. fumigatus (91%) [ 13 ].…”

Section: Discussionmentioning

confidence: 97%

Chronic Pulmonary Aspergillosis in Post Tuberculosis Patients in Indonesia and the Role of LDBio Aspergillus ICT as Part of the Diagnosis Scheme

Rozaliyani

Rosianawati

Handayani

et al. 2020

JoF

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Chronic pulmonary aspergillosis (CPA) is a common sequela of pulmonary tuberculosis (TB). The diagnosis of CPA is difficult and often misdiagnosed as smear-negative TB in endemic settings. Aspergillus IgG detection is the cornerstone of CPA diagnosis. There are a lack of studies on the prevalence of CPA in GeneXpert/smear-negative TB patients in Indonesia, despite a high number of TB cases. This study aims to determine the CPA rate in HIV-negative, GeneXpert-negative patients presenting with symptoms following completion of TB therapy and to evaluate the performance of LDBio Aspergillus immunochromatographic technology (ICT) lateral flow assay in the diagnosis of CPA. CPA was diagnosed on the basis of symptoms for ≥3 months, characteristic chest imaging and positive Aspergillus culture. Twenty (22%) out of 90 patients met the criteria for CPA. The LDBio test was positive in 16 (80%) CPA patients and in 21 (30%) non-CPA patients (p < 0.001) with 80% sensitivity and 70% specificity. Logistic regression revealed a positive LDBio Aspergillus ICT result, smoking history and diabetes to be important predictors of CPA diagnosis. Although CPA is an unrecognised disease in Indonesia, this study suggests that more than one in five GeneXpert negative patients with persistent symptoms following completion of TB therapy may have CPA.

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Section: Discussionmentioning

confidence: 97%

Chronic Pulmonary Aspergillosis in Post Tuberculosis Patients in Indonesia and the Role of LDBio Aspergillus ICT as Part of the Diagnosis Scheme

Rozaliyani

Rosianawati

Handayani

et al. 2020

JoF

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“…A. fumigatus, Aspergillus niger, H. capsulatum, C. neoformans and C. albicans are often responsible for causing severe secondary infections in tuberculosis patients. (10,56,58,59) Reactivation of latent cytomegalovirus (CMV) can lead to the development of concomitant or sequential fungal infections in immunosuppressed patients. Both solid organ and autologous stem cell transplant recipients, with a history of CMV infection, have higher risk for developing IFD.…”

Section: Invasive Candidiasismentioning

confidence: 99%

“…A. fumigatus , Aspergillus niger , H. capsulatum , C. neoformans and C. albicans are often responsible for causing severe secondary infections in tuberculosis patients. 10 , 56 , 58 , 59 …”

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confidence: 99%

Invasive fungal disease in humans: are we aware of the real impact?

Firacative

2020

Mem. Inst. Oswaldo Cruz

129

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Despite the medical advances and interventions to improve the quality of life of those in intensive care, people with cancer or severely immunocompromised or other susceptible hosts, invasive fungal diseases (IFD) remain severe and underappreciated causes of illness and death worldwide. Therefore, IFD continue to be a public health threat and a major hindrance to the success of otherwise life-saving treatments and procedures. Globally, hundreds of thousands of people are affected every year with Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, Pneumocystis jirovecii, endemic dimorphic fungi and Mucormycetes, the most common fungal species causing invasive diseases in humans. These infections result in morbidity and mortality rates that are unacceptable and represent a considerable socioeconomic burden. Raising the general awareness of the significance and impact of IFD in human health, in both the hospital and the community, is hence critical to understand the scale of the problem and to raise interest to help fighting these devastating diseases.

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“…The main risk factors of primary and secondary fungal pulmonary coinfection in TB patients include immunodeficiency due to mucosal or cutaneous barrier disruption, using broad-spectrum antibiotics and steroids, premature birth, hemodialysis, cytotoxic chemotherapy, tissue transplantation, chronic diseases, neutropenia, defects in the function of innate or cell-mediated immunity, metabolic syndrome, solid and hematologic malignancies, advanced age , recent radiation therapy, burns, prolonged hospitalization, severe trauma, bacterial infections, recent surgery, and implanted central intravascular access devices [7].…”

Section: Introductionmentioning

confidence: 99%

Candida Coinfection among Patients with Pulmonary tuberculosis in the World; A Systematic Review and Meta-analysis of Cross-Sectional Studies

Ghazalibina¹,

Shakerimoghaddam²,

Khaledi³

2019

Preprint

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Background Diagnosis of fungal co-infections in patients with pulmonary tuberculosis has critical importance. In this review, we aimed to determine the prevalence of candida coinfection in patients with pulmonary tuberculosis.Methods The present systematic review of cross-sectional studies was conducted based on the Preferred Reporting Items for Systematic reviews and Meta-analysis (PRISMA) Protocol. Studies published online in English from January 2001 to March 2019 were assessed. Literature search was performed in Web of Science, MEDLINE/PubMed, and Scopus databases using keywords combinations of “pulmonary fungal”, “pulmonary coinfection”, OR “pulmonary mycosis”, “pulmonary fungal infections/agents”, OR “polymicrobial infection”, OR “secondary infection”, OR “mixed infections”, “pulmonary candidiasis”, “fungi coinfection”, “fungal co-colonization”, AND “pulmonary tuberculosis”, OR “pulmonary TB”. Data was analyzed using Comprehensive Meta-Analysis software. Heterogeneity between studies was evaluated by Cochran's Q, and I 2 tests.Results The pooled global prevalence of candida coinfection among patients with pulmonary tuberculosis was 25.7% (95% CI: 23.7-27.9). C. albicans was the most prevalent Candida spp. with a pooled prevalence of 65.8% (95% CI: 54.3-75.7). Risk factors of candida coinfection included smoking, diabetes, advanced age, and low body mass index.Conclusion The present review showed the high rate of candida coinfection among patients suffering from pulmonary tuberculosis. Adequate measures are necessary to early diagnose and treat these infections.

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Microarray detection of fungal infection in pulmonary tuberculosis

Cited by 21 publications

References 20 publications

Chronic Pulmonary Aspergillosis in Post Tuberculosis Patients in Indonesia and the Role of LDBio Aspergillus ICT as Part of the Diagnosis Scheme

Chronic Pulmonary Aspergillosis in Post Tuberculosis Patients in Indonesia and the Role of LDBio Aspergillus ICT as Part of the Diagnosis Scheme

Invasive fungal disease in humans: are we aware of the real impact?

Candida Coinfection among Patients with Pulmonary tuberculosis in the World; A Systematic Review and Meta-analysis of Cross-Sectional Studies

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