Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension

Miao, Ran; Wang, Ying; Wan, Jun; Dong, Liubing; Gong, Juanni; Li, Jifeng; Liang, Yan; Zhai, Zhenguo; Yang, Yuanhua

doi:10.1097/md.0000000000007354

Cited by 63 publications

(63 citation statements)

References 40 publications

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“…However, due to the advances in biological technology, especially high-throughput sequencing technology and bioinformatics, their abundance in diverse cells has recently been discovered. [25][26][27][28] A number of studies have reported that circRNAs play a critical role in many diseases, [8][9][10][11] including cancers. [12][13][14][15] However, the functions of circRNAs in CRC are unclear.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 Emerging studies have revealed numerous potential functions of circRNAs including acting as microRNA (miRNA) sponges, regulating transcription and splicing, sequestrating RNA binding proteins, and protein-coding capacity. [4][5][6][7] Based on the above functions of circRNAs above, a number of studies have revealed that circRNAs correlate with diverse diseases, [8][9][10][11] including cancers. [12][13][14][15] Thus, circRNAs hold the potential to function as therapeutic targets and diagnostic biomarkers of cancers.…”

Section: Introductionmentioning

confidence: 99%

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<p>Circular RNA circHUWE1 Is Upregulated and Promotes Cell Proliferation, Migration and Invasion in Colorectal Cancer by Sponging miR-486</p>

Chen

et al. 2020

OTT

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Background: Emerging studies have revealed that circular RNAs (circRNAs) correlate with diverse diseases including cancers. However, little is known about the functions of circRNAs in colorectal cancer (CRC). In our previous research, downregulation of hsa_circ_0140388 (circHUEW1) has been detected in CRC tissues through highthroughput sequencing. However, the underlying mechanism by which circHUWE1 regulates the proliferation and apoptosis in CRC has not been investigated. Materials and Methods: The levels of circHUWE1 in 58 pairs of CRC tissues and corresponding adjacent healthy tissues were detected by RT-qPCR. In addition, the effects of circHUWE1 on cell proliferation, apoptosis migration and invasion were evaluated by cell proliferation assays, flow cytometry, and transwell assays in HCT116 and SW480 cell lines respectively. Meanwhile, the dual-luciferase reporter system assay was used to explore the interaction between circHUWE1 and miR-486 (hsa-miR-486-5p). Results: In this study, we demonstrate that the expression of circHUEW1 is upregulated in CRC tissues. High expression of circHUEW1 was significantly associated with lymphovascular invasion (P =0.036), lymph node metastasis (P =0.017), distant metastasis (P =0.024), and TNM stage (P =0.009). Moreover, the area under the curve (AUC) of the receiver operating characteristic (ROC) curve was 0.732, which indicated that circHUWE1 could serve as a potential biomarker in the detection of CRC. Silencing circHUWE1 significantly inhibited the proliferation, migration and invasion capacity of CRC cells in vitro. Mechanistically, we demonstrated that circHUWE1 could sponge miR-486 and the downregulation of miR-486 could reverse the cancer suppressive effects caused by silencing circHUWE1. Conclusion: In this study, our results revealed that circHUWE1 may be a potential therapeutic target and diagnostic biomarker for CRC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Circular RNA circHUWE1 Is Upregulated and Promotes Cell Proliferation, Migration and Invasion in Colorectal Cancer by Sponging miR-486</p>

Chen

et al. 2020

OTT

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“…Numerous publications have demonstrated that aberrant expression of circRNAs has been shown to occur in various human diseases. Studies reported the association of hsa_circ_0002062 with pulmonary hypertension, the increased expression of hsa_circRNA_104871 with rheumatoid arthritis diagnosis and HIF1α‐associated circRNA promoting the proliferation of breast cancer cells . These findings indicate that circRNAs may be significant biological molecules to understand disease mechanisms and to identify biomarkers for disease diagnosis and therapy.…”

Section: Introductionmentioning

confidence: 99%

Dysregulated circRNAs in plasma from active tuberculosis patients

Gao

et al. 2018

J Cellular Molecular Medi

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Endogenous circular RNAs (circRNAs) have been reported in various diseases. However, their role in active TB remains unknown. The study was aimed to determine plasma circRNA expression profile to characterize potential biomarker and improve our understanding of active TB pathogenesis. CircRNA expression profiles were screened by circRNA microarrays in active TB plasma samples. Dysregulated circRNAs were then verified by qRT‐PCR. CircRNA targets were predicted based on analysis of circRNA‐miRNA‐mRNA interaction. GO and KEGG pathway analyses were used to predict the function of circRNA. ROC curve was calculated to evaluate diagnostic value for active TB. A total of 75 circRNAs were significantly dysregulated in active TB plasma. By further validation, hsa_circRNA_103571 exhibited significant decrease in active TB patients and showed potential interaction with active TB‐related miRNAs such as miR‐29a and miR‐16. Bioinformatics analysis revealed that hsa_circRNA_103571 was primarily involved in ras signalling pathway, regulation of actin cytoskeleton, T‐ and B‐cell receptor signalling pathway. ROC curve analysis suggested that hsa_circRNA_103571 had significant value for active TB diagnosis. Circulating circRNA dysregulation may play a role in active TB pathogenesis. Hsa_circRNA_103571 may be served as a potential biomarker for active TB diagnosis, and hsa_circRNA_103571‐miRNA‐mRNA interaction may provide some novel mechanism for active TB.

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“…To explore the function of lncRNAs in the ceRNA network, we performed enrichment analyses of the DEmRNAs involved in the network to reveal the potential biological functions and pathways ( p-value < 0.05) [20] in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and then visualized the results using the clusterPro ler package in R [21,22].…”

Section: Functional Enrichment Analysesmentioning

confidence: 99%

Construction of a lncRNA-Associated ceRNA Network and Identification of A Potential Six-lncRNA Prognostic Signature for Esophageal Cancer

Wang

Liu

et al. 2020

Preprint

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BACKGROUND: Mounting evidence has shown that long noncoding RNAs (lncRNAs) can function as competing endogenous RNAs (ceRNAs) which participate in the initiation and progression of cancers. In the ceRNA network, lncRNAs, microRNAs (miRNAs) and mRNAs, communicate with and co-regulate each other. Rarely there is a systematic lncRNA-mediated ceRNA network and potential specific ceRNA pairs or triples of esophageal cancer (EC). In this study, we investigate the lncRNA-mediated ceRNA network in EC and screen the potential prognostic lncRNA biomarkers.METHODS: We obtained mRNA, miRNA, and lncRNA expression data and relevant clinical features on patients with EC from The Cancer Genome Atlas (TCGA), and used the edgR package to identify differentially expressed mRNAs, lncRNAs and miRNAs between EC samples and normal samples. The EC ceRNA network was constructed based on miRNA target prediction through the databases of miRcode, miRDB, miRTarBase and TargetScan. And then Pearson’s correlation analysis was adopted to identify co-expression mRNA-lncRNA pairs. Finally, the robust likelihood-based survival analysis and Cox regression models were used to identify prognosis-related lncRNAs, which was evaluated by Kaplan-Meier and receiver operating characteristic (ROC) curve analysis.RESULTS: A total of 3,200 mRNAs, 131 miRNAs and 1,338 lncRNAs were identified as significantly differentially expressed in EC, of which, 30 mRNAs, 15 lncRNAs, and 8 miRNAs were incorporated in the ceRNA network. According to the ceRNA network node degrees, lncRNA MAGI2-AS3, hsa-mir-93 and TGFBR2 were the key genes. Also, the ceRNA network revealed some important ceRNA pairs and triples, such as SNX29P2-TGFBR2 and MAGI2-AS-hsa-mir-143-COL1A1. Finally, we developed a six-lncRNA signature (ZNF341-AS1, AC130324.2, AC027271.1, AL591212.1, AL732314.4 and LOC105372352), with improved diagnostic potential for EC with the area under the ROC curve of 0.93.CONCLUSIONS: our present work sheds new light on the tumorigenesis roles of lncRNA-mediated ceRNA network in EC and identifies a six‐lncRNA model that could be used as candidate prognostic signature.

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Microarray expression profile of circular RNAs in chronic thromboembolic pulmonary hypertension

Abstract: Supplemental Digital Content is available in the text

Cited by 63 publications

References 40 publications

<p>Circular RNA circHUWE1 Is Upregulated and Promotes Cell Proliferation, Migration and Invasion in Colorectal Cancer by Sponging miR-486</p>

<p>Circular RNA circHUWE1 Is Upregulated and Promotes Cell Proliferation, Migration and Invasion in Colorectal Cancer by Sponging miR-486</p>

Dysregulated circRNAs in plasma from active tuberculosis patients

Construction of a lncRNA-Associated ceRNA Network and Identification of A Potential Six-lncRNA Prognostic Signature for Esophageal Cancer

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