Microarray expression profiles of genes in lung tissues of rats subjected to focal cerebral ischemia-induced lung injury following bone marrow-derived mesenchymal stem cell transplantation

Abstract

Ischemia-induced stroke is the most common disease of the nervous system and is associated with a high mortality rate worldwide. Cerebral ischemia may lead to remote organ dysfunction, particular in the lungs, resulting in lung injury. Nowadays, bone marrow-derived mesenchymal stem cells (BMSCs) are widely studied in clinical trials as they may provide an effective solution to the treatment of neurological and cardiac diseases; however, the underlying molecular mechanisms remain unknown. In this study, a model of permanent focal cerebral ischemia-induced lung injury was successfully established and confirmed by neurological evaluation and lung injury scores. We demonstrated that the transplantation of BMSCs (passage 3) via the tail vein into the lung tissues attenuated lung injury. In order to elucidate the underlying molecular mechanisms, we analyzed the gene expression profiles in lung tissues from the rats with focal cerebral ischemia and transplanted with BMSCs using a Gene microarray. Moreover, the Gene Ontology database was employed to determine gene function. We found that the phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, transforming growth factor-β (TGF-β) and platelet-derived growth factor (PDGF) were downregulated in the BMSC transplantation groups, compared with the control group. These results suggested that BMSC transplantation may attenuate lung injury following focal cerebral ischemia and that this effect is associated with the downregulation of TGF-β, PDGF and the PI3K-AKT pathway.

MiR224-5p Inhibitor Restrains Neuronal Apoptosis by Targeting NR4A1 in the Oxygen-Glucose Deprivation (OGD) Model

“…As previously reported, stroke could lead to neurological dysfunction (Sun et al, 2010;Hu et al, 2017;Jain et al, 2017), and the process of stroke had the participation of neuronal apoptosis (Li et al, 1999). In this study, we found that brain infarction was obvious with TTC staining and Longa score confirmed the neurological deficit in MCAO rats.…”

Microarray Expression Profiles of lncRNAs and mRNAs in Postoperative Cognitive Dysfunction

“…Microarray was used to investigate differentially expressed lncRNAs and mRNAs which then become the main data for the subsequent bioinformatics analyses of the study. This working strategy has been applied and validate in studying many diseases including cancer ( Han et al, 2018 ; Liang et al, 2018 ; Lou et al, 2018 ), cerebral ischemia-induced lung injury ( Hu et al, 2017 ), and traumatic brain injury ( Lyu et al, 2017 ). In our study, microarray was used to study the changes in expression of lncRNAs and mRNAs in POCD group.…”

“…GO analysis has illuminated the functions of genes through biological process, cellular component, and molecular function. While KEGG is normally used to study the possible roles of expressed RNA with correlation to cell signaling pathways ( Hu et al, 2017 ).…”

Screening Key Pathogenic Genes and Small Molecule Compounds for PNET

“…This technology can quickly and accurately analyze high-level information, and it provides the important theoretical basis for sequence analysis, gene expression, and genome research. 7 In recent years, various public free chip databases can be used by researchers on the Internet for in-depth research. However, single PNET chip analysis may result in statistical deviation, and then lead to results that are not accurate enough.…”

“…At present, gene chip technology has been widely used in the diagnosis and treatment of diseases. This technology can quickly and accurately analyze high-level information, and it provides the important theoretical basis for sequence analysis, gene expression, and genome research 7. In recent years, various public free chip databases can be used by researchers on the Internet for in-depth research.…”