Microarray fabrication techniques for multiplexed bioassay applications

Aggarwal, Roshan Tosh; Lai, Leyun; Li, Huiyan

doi:10.1016/j.ab.2023.115369

Cited by 2 publications

(2 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More detailed SEM images reveal small defects on the 30 × 30 μm PDMS replica ( Figure 1 , bottom image), which may have already occurred during the photoresist pattern production process or casting. These defects can be caused by several factors, such as insufficient or too long exposure time or poor removal of the photoresist when a residual layer remains [ 86 , 87 ]. Tiny defects were observed to a much lesser extent on the 50 × 50 μm 2 sample, indicating that their formation had already occurred before PDMS casting.…”

Section: Resultsmentioning

confidence: 99%

“…Microfluidic applications [67,68] PE CVD Applications that require stable hydrophilic surface property in PDMS [69] Photografting of PEG Protein adsorption and cell adhesion [71] Imidazolium antimicrobial compound Catheter fabrication [73] UV-light curing Drug delivery [75] Soft lithography Replication of cardiovascular flow conditions [80] Bulk oxygen, nitrogen, or argon + collagen Adhesive free skin substitutes [82] Bulk plasma treatment and collagen Bone marrow-derived stromal cells [83] Hot embossing Photonic devices [85][86][87] Different bulk/ surface treatments Biomedical applications [88,89] Surface plasma treatment and collagen Mesenchymal stem cells [90] Different bulk/surface treatments Wettability and recyclability applications [91][92][93] Plasma/collagen treatment Tissue engineering-different cell types [94][95][96][97][98] Bulk modification Cell mechanobiology in muscle and nerve [99] The promising results of this study suggest the potential for reconstructing threedimensional vascularized tissue suitable for implantation. Photolithographic masks were designed to emulate the shape, size, and orientation of muscle fibers.…”

Section: Plasma Treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Slepičková Kasálková,

Juřicová,

Fajstavr

et al. 2024

IJMS

View full text Add to dashboard Cite

We focused on polydimethylsiloxane (PDMS) as a substrate for replication, micropatterning, and construction of biologically active surfaces. The novelty of this study is based on the combination of the argon plasma exposure of a micropatterned PDMS scaffold, where the plasma served as a strong tool for subsequent grafting of collagen coatings and their application as cell growth scaffolds, where the standard was significantly exceeded. As part of the scaffold design, templates with a patterned microstructure of different dimensions (50 × 50, 50 × 20, and 30 × 30 μm2) were created by photolithography followed by pattern replication on a PDMS polymer substrate. Subsequently, the prepared microstructured PDMS replicas were coated with a type I collagen layer. The sample preparation was followed by the characterization of material surface properties using various analytical techniques, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). To evaluate the biocompatibility of the produced samples, we conducted studies on the interactions between selected polymer replicas and micro- and nanostructures and mammalian cells. Specifically, we utilized mouse myoblasts (C2C12), and our results demonstrate that we achieved excellent cell alignment in conjunction with the development of a cytocompatible surface. Consequently, the outcomes of this research contribute to an enhanced comprehension of surface properties and interactions between structured polymers and mammalian cells. The use of periodic microstructures has the potential to advance the creation of novel materials and scaffolds in tissue engineering. These materials exhibit exceptional biocompatibility and possess the capacity to promote cell adhesion and growth.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Plasma Treatmentmentioning

confidence: 99%

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Slepičková Kasálková,

Juřicová,

Fajstavr

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Concave Magnetic-Responsive Hydrogel Discs for Enhanced Bioassays

Ghaffarzadeh Bakhshayesh,

2024

Biosensors

View full text Add to dashboard Cite

Receptor-based biosensors often suffer from slow analyte diffusion, leading to extended assay times. Moreover, existing methods to enhance diffusion can be complex and costly. In response to this challenge, we presented a rapid and cost-effective technique for fabricating concave magnetic-responsive hydrogel discs (CMDs) by straightforward pipetting directly onto microscope glass slides. This approach enables immediate preparation and customization of hydrogel properties such as porosity, magnetic responsiveness, and embedded particles and is adaptable for use with microarray printers. The concave design increased the surface area by 43% compared to conventional hemispherical hydrogels, enhancing diffusion rates and accelerating reactions. By incorporating superparamagnetic particles, the hydrogels become magnetically responsive, allowing for stirring within reagent droplets using magnets to improve mixing. Our experimental results showed that CMDs dissolved approximately 2.5 times faster than hemispherical ones. Numerical simulations demonstrated up to a 46% improvement in diffusion speed within the hydrogel. Particles with lower diffusion coefficients, like human antibodies, benefited most from the concave design, resulting in faster biosensor responses. The increased surface area and ease of fabrication make our CMDs efficient and adaptable for various biological and biomedical applications, particularly in point-of-care diagnostics where rapid and accurate biomarker detection is critical.

show abstract

Microarray fabrication techniques for multiplexed bioassay applications

Cited by 2 publications

References 76 publications

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Concave Magnetic-Responsive Hydrogel Discs for Enhanced Bioassays

Contact Info

Product

Resources

About