Microarray-in-a-tube system in the function of detection human papillomavirus genotypes

Abstract

Patients infected human papillomavirus (HPV) is increasing all over the world, in particular the community of woman. Microarray-in-a-tube system is a novel method of integrating polymerase chain reaction (PCR) with the microarray technique and a simple and high-throughput detection system. In this work, the microarray-in-a-tube system used in the function of detection human papillomavirus genotypes. Detection human papillomavirus genotypes of a microarray-in-a -tube system was composed of the preparation arrays, PCR, hybridization on chip, fluorescence detection, containing general GP5+/GP6+ premier, 30 targets. These results suggest that signal-noise ratio (SNR) of this system was set as SNR ≥ 2.0 at 100 copies. The system was suitable for other disease diagnosis in future.