Microarray profiling of gene expression patterns in glomerular cells of astaxanthin-treated diabetic mice: A nutrigenomic approach

Naito, Yuji; Uchiyama, Kazuhiko; Mizushima, Katsura; Kuroda, Masaaki; Akagiri, Satomi; Takagi, Tomohisa; Handa, Osamu; Kokura, Satoshi; Yoshida, Norimasa; Ichikawa, Hiroshi; Takahashi, Jun; Yoshikawa, Toshikazu

doi:10.3892/ijmm.18.4.685

Cited by 14 publications

(19 citation statements)

References 20 publications

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“…Evidence of renal oxidative stress in db/db mice has been shown previously in reports of increased kidney lipid peroxidation (22), nitrotyrosine expression (13), and decreased glutathione levels in renal cortex (13). In addition, treatment of db/db mice with astaxanthine, which exhibits antioxidant activity, significantly reduced the diabetes-induced increase in mesangial area and urinary albumin (27). Considered together, these reports and our data indicate an adaptive renal response to increased oxidative stress occurs in diabetes.…”

Section: Discussionsupporting

confidence: 73%

“…Blot was reprobed for ␤-actin to demonstrate equal loading of protein between lanes. identified in the proteome in 13-wk-old db/db mice (27). Although concurrent reports for gene expressions of these 12 proteins in this age group are not known, protein expression of 3 of the proteins is upregulated in db/db mice used in the present study.…”

Section: Discussionmentioning

confidence: 86%

“…The present study is the first to analyze global protein expression changes in glomeruli with diabetes. Genomic expression profiling of glomeruli from 5-to 7-wk-old (23) and 13-wk-old (27) db/db mice has identified 116 and 779 differentially regulated genes, and 343 genes of kidneys from 150-day-old db/db mice were regulated (40). In addition, renal cortex from db/db mice demonstrates differential expression of genes during initial and overt phases of DN (24).…”

Section: Discussionmentioning

confidence: 97%

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Proteomic analysis defines altered cellular redox pathways and advanced glycation end-product metabolism in glomeruli ofdb/dbdiabetic mice

Barati

Merchant²,

Kain³

et al. 2007

American Journal of Physiology-Renal Physiology

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To attain a profile of protein expression during diabetes, we applied proteomic analysis to glomeruli of 160-day-old db/db diabetic and db/m nondiabetic mice. Glomerular proteins were extracted and separated by two-dimensional gel electrophoresis to construct a proteome map. Matrix-assisted laser desorption and ionization-time of flight mass spectrometry and peptide mass fingerprinting were used to identify 190 proteins. Of 105 analyzed spots, expression of 40 proteins, including the antioxidative enzymes peroxiredoxin 1 and 3, glutathione peroxidase 1, and SOD-1, was increased with diabetes, suggesting an adaptive response to oxidative stress associated with this diabetic model. However, activity of glutathione peroxidase and SOD was unaltered in glomeruli of diabetic mice. Expression of glyoxalase I was increased in glomeruli of diabetic mice. Because the cofactor for glyoxalase I, glutathione, is decreased in renal cortex of db/db mice, renal cortical glyoxalase I activity was measured in vitro with fixed amounts of exogenous glutathione. Glyoxalase I activity was decreased in renal cortex of db/db mice. These data indicate that diabetes-induced decreases in glyoxalase I activity are likely to be due to glutathione-dependent and -independent mechanisms and that increased expression of glyoxalase I may represent an insufficient adaptive response to increased methylglyoxal formation.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 97%

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Proteomic analysis defines altered cellular redox pathways and advanced glycation end-product metabolism in glomeruli ofdb/dbdiabetic mice

Barati

Merchant²,

Kain³

et al. 2007

American Journal of Physiology-Renal Physiology

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“…In addition, the importance of the transforming growth factor-b signaling in the pathophysiology of diabetic nephropathy was confirmed by Naito et al [67], who determined the gene expression patterns in the glomerular cells of the diabetic mouse kidney and investigated the effects of astaxanthin on the expression of these genes, and found that long-term treatment with astaxanthin significantly decreased the expression of upregulated probes, including those genes associated with oxidative phosphorylation, oxidative stress, and the transforming growth factor-bcollagen synthesis system.…”

Section: Diabetic Nephropathymentioning

confidence: 53%

Potential health‐promoting effects of astaxanthin: A high‐value carotenoid mostly from microalgae

Yuan

Peng

Yin

et al. 2010

Molecular Nutrition Food Res

555

309

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The ketocarotenoid astaxanthin can be found in the microalgae Haematococcus pluvialis, Chlorella zofingiensis, and Chlorococcum sp., and the red yeast Phaffia rhodozyma. The microalga H. pluvialis has the highest capacity to accumulate astaxanthin up to 4-5% of cell dry weight. Astaxanthin has been attributed with extraordinary potential for protecting the organism against a wide range of diseases, and has considerable potential and promising applications in human health. Numerous studies have shown that astaxanthin has potential health-promoting effects in the prevention and treatment of various diseases, such as cancers, chronic inflammatory diseases, metabolic syndrome, diabetes, diabetic nephropathy, cardiovascular diseases, gastrointestinal diseases, liver diseases, neurodegenerative diseases, eye diseases, skin diseases, exercise-induced fatigue, male infertility, and HgCl₂-induced acute renal failure. In this article, the currently available scientific literature regarding the most significant activities of astaxanthin is reviewed.

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“…Naito et al [64] compared gene expression of glomerular cells harvested from kidneys of db/db and db/m mice by laser capture microdissection. They then performed pathway analysis of 550 upregulated probes using the Ingenuity signal analysis.…”

Section: Serial Analysis Of Gene Expression and Dna Microarraymentioning

confidence: 99%

Discovery of Genes Related to Diabetic Nephropathy in Various Animal Models by Current Techniques

Wada

Sun²,

Kanwar³

2011

Contributions to Nephrology

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One of the major problems facing clinical nephrology currently throughout the world is an exponential increase in patients with end-stage renal disease (ESRD), which is largely related to a high incidence of diabetic nephropathy. The latter is characterized by a multitude of metabolic and signaling events following excessive channeling of glucose, which leads to an increased synthesis of extracellular matrix (ECM) glycoproteins resulting in glomerulosclerosis, interstitial fibrosis and ultimately ESRD. With the incidence of nephropathy at pandemic levels and a high rate of ESRD, physicians around the world must treat a disproportionately large number of diabetic patients with up-to-date innovative measures. In this regard, identification of genes that are crucially involved in the progression of diabetic nephropathy would enhance the discovery of new biomarkers and could also promote the development of novel therapeutic strategies. Over the last decade, we focused on the recent methodologies of high-throughput and genome-wide screening for identification of relevant genes in various animal models, which included the following: (1) single nucleotide polymorphism-based genome-wide screening; (2) the transcriptome approach, such as differential display reverse transcription polymerase chain reaction (DDRT-PCR), representational difference analysis of cDNA (cDNA-RDA)/suppressive subtractive hybridization, SAGE (serial analysis of gene expression) and DNA Microarray; and (3) the proteomic approach and 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectroscopic analysis. Several genes, such as Tim44 (translocase of inner mitochondrial membrane-44), RSOR/MIOX (renal specific oxidoreductase/myo-inositol oxygenase), UbA52, Rap1b (Ras-related GTPase), gremlin, osteopontin, hydroxysteroid dehydrogenase-3β isotype 4 and those of the Wnt signaling pathway, were identified as differentially expressed genes in kidneys of diabetic rodents. Functional analysis of these genes and the subsequent translational research in the clinical settings would be very valuable in the prevention and treatment of diabetic nephropathy. Future trends for identification of the biomarkers and therapeutic target genes should also include genome scale DNA/histone-methylation profiling, metabolomic approaches (e.g. metabolic phenotyping by 1H spectroscopy) and lectin microarray for glycan profiling along with the development of robust data-mining strategies.

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Microarray profiling of gene expression patterns in glomerular cells of astaxanthin-treated diabetic mice: A nutrigenomic approach

Cited by 14 publications

References 20 publications

Proteomic analysis defines altered cellular redox pathways and advanced glycation end-product metabolism in glomeruli ofdb/dbdiabetic mice

Proteomic analysis defines altered cellular redox pathways and advanced glycation end-product metabolism in glomeruli ofdb/dbdiabetic mice

Potential health‐promoting effects of astaxanthin: A high‐value carotenoid mostly from microalgae

Discovery of Genes Related to Diabetic Nephropathy in Various Animal Models by Current Techniques

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