Microarray Spotter and Printing Technologies

Datar, Akshata; Lee, Dong Woo; Jeon, Sang Youl; Lee, Moo‐Yeal

doi:10.1007/978-3-319-46805-1_2

Cited by 4 publications

(6 citation statements)

References 19 publications

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“…Clogging causes interruption in flow and consequent unevenness in the print, and may require the replacement of the nozzle during a print. 12,13…”

Section: Introductionmentioning

confidence: 99%

“…Clogging causes interruption in flow and consequent unevenness in the print, and may require the replacement of the nozzle during a print. 12,13 The parameter space involved in bioprinting is large and many factors contribute to preventing clogging and achieving a successful print. Leaving aside biological considerations, such as the presence and survival of cells, bioinks must have proper rheological properties to flow through small channels without clogging while also providing sufficient structural support to retain an appropriate shape upon leaving the channel.…”

Section: Introductionmentioning

confidence: 99%

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In situ polymer gelation in confined flow controls intermittent dynamics

Smith,

Hashmi

2024

Soft Matter

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“…Clogging causes interruption in flow and consequent unevenness in the print, and may require the replacement of the nozzle during a print. 12,13…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In situ polymer gelation in confined flow controls intermittent dynamics

Smith,

Hashmi

2024

Soft Matter

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show abstract

“…An example of failure is nozzle clogging, where some obstruction prevents the flow of ink through the nozzle. Clogging causes interruption in flow and consequent unevenness in the print, and may require the replacement of the nozzle during a print [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…Aside from the rheology of the solution, clogs can also be caused by cells which stick to the wall of a nozzle and accumulate to block the channel [19][20][21]. This is a particular problem in cases of inhomogeneity in the ink or in situations where the particles readily sediment [13,20,22,23]. In both of these effects, the nature and degree of polymer cross-linking strongly influence the rheology and homogeneity of the ink.…”

Section: Introductionmentioning

confidence: 99%

In situ polymer gelation in confined flow controls intermittent dynamics

Smith,

Hashmi

2023

Preprint

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Polymer flows through pores, nozzles and other small channels govern engineered and naturally occurring dynamics in many processes, from 3D printing to oil recovery in the earth’s subsurface to a wide variety of biological flows. The crosslinking of polymers can change their material properties dramatically. In engineered flows, polymer crosslinking is often a situation to be avoided. For instance, in 3D printing it is greatly preferred for crosslinking to occur upon impact with a substrate rather than prior to exiting a nozzle. In blood flow, however, polymer crosslinking can either be advantageous, as in wound healing, or pathological, as in thromboembolism formation. In either of these situations, and others, it is advantageous to know a priori whether or not crosslinking polymers will lead to clogged channels or cessation of flow. In this study, we investigate the flow of a common biopolymer, alginate, while it undergoes crosslinking by the addition of a crosslinker, calcium, driven through a microfluidic channel at constant flow rate. While quantifying the limits on flowability and clogging in situ in this crosslinking polymer system, we observe a remarkable phenomenon in which the crosslinked polymer intermittently clogs the channel. We observe a pattern of deposition and removal of a crosslinked gel that is simultaneously highly reproducible, long-lasting and controllable by a variety of parameters. We map this behavior as a function of flow rate, polymer concentration, and crosslinker concentration. Our results pave the way for future investigations of the dynamics and control of intermittent flows in crosslinking polymers.

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“…Aside from the rheology of the solution, clogs can also be caused by cells which stick to the wall of a nozzle and accumulate to block the channel [17][18][19]. This is a particular problem in cases of inhomogeneity in the ink or where the particles readily sediment [18,[20][21][22]. Finally, evaporation of solvent at the nozzle outlet can cause precipitation of the polymers and cells, the build-up of which can block flow [19,23,24].…”

Section: Introductionmentioning

confidence: 99%

In situ gelation in confined flow controls intermittent dynamics

Smith,

Hashmi

2023

Preprint

View full text Add to dashboard Cite

Polymer flows through pores, nozzles and other small channels govern both engineered and naturally occurring dynamics in many processes, from 3D printing to oil recovery in the earth's subsurface to a wide variety of biological flows. The crosslinking of polymers can change their material properties dramatically. In engineered flows, polymer crosslinking is often a situation to be avoided. For instance, in 3D printing it is greatly preferred for crosslinking to occur upon impact with a substrate rather than prior to exiting a nozzle. In blood flow, on the other hand, polymer crosslinking can either be advantageous, as in wound healing, or pathological, as in thromboembolism formation. In either of these situations, and in others, it is advantageous to know a priori whether or not crosslinking polymers will lead to clogged channels or cessation of flow. In this study, we investigate the flow of a common biopolymer, alginate, while it undergoes crosslinking by the addition of a crosslinker, calcium, driven through a microfluidic channel at constant flow rate. In addition to quantifying the limits on flowability and clogging in situ in this crosslinking polymer system, we observe a remarkable phenomenon in which the crosslinked polymer intermittently clogs the channel. We observe a pattern of deposition and removal of a crosslinked gel that is simultaneously highly reproducible, long-lasting and controllable by a variety of parameters. We map this behavior as a function of flow rate, polymer concentration, and crosslinker concentration. Our results pave the way for future investigations of the phase diagram controlling intermittent flows in crosslinking polymer systems. Furthermore, the dynamics of the deposition and ablation process suggests an intriguing possibility that the route to clogging failure exhibits signatures of chaotic dynamics.

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Microarray Spotter and Printing Technologies

Cited by 4 publications

References 19 publications

In situ polymer gelation in confined flow controls intermittent dynamics

In situ polymer gelation in confined flow controls intermittent dynamics

In situ polymer gelation in confined flow controls intermittent dynamics

In situ gelation in confined flow controls intermittent dynamics

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