Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

Licker, Monica; Anghel, Andrei; Moldovan, Roxana; Hogea, Elena; Muntean, Delia; Horhat, Florin George; Șeclăman, Edward; Tamas, L.; Anghel, Mariana; Bădițoiu, Luminița

doi:10.37358/rc.17.11.5925

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…In the Gram-positive group, a higher degree of resistance of MRSA was found to be against penicillin (95.37%), erythromycin (79.62%), clindamycin (78.70%), tetracycline (70.37%) and ciprofloxacin (65.74%), consistent with other findings [1,10]. A prospective study performed in Romania by Licker at al, identified 66.51% MDR and 20.18% XDR S.aureus strains [24], in the conditions in which MRSA has been the most important cause of antimicrobial-resistant healthcare-associated infections worldwide, with higher percentages in the southern and south-eastern parts of Europe [15]. The most active antibiotic against MRSA (table 5) was linezolid, with almost all strains (105) being susceptible (table 5).…”

Section: Resultssupporting

confidence: 82%

Antibiotic Resistance Pattern of Bacterial Pathogens in Elderly Patients Admitted in the Intensive Care Unit

Golli¹,

Niţu²,

Bălăşoiu³

et al. 2019

Rev. Chim.

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To identify and to determine the resistance pattern of bacterial pathogens involved in infections of the elderly patients (� 65 years) admitted in the intensive care unit (ICU) at County Emergency Clinical Hospital Craiova, Romania. A retrospective study of bacterial pathogens was carried out on 463 elderly patients (� 65 years) admitted to the ICU, from January to December 2017. The analysis of the resistance patterns for the action of the appropriate antibiotics was performed using Vitek 2 Compact system and diffusion method. In this study there were analyzed 617 samples from 463 elderly patients (� 65 years). A total of 776 bacterial isolates were obtained, of which 175 strains of Klebsiella spp. (22.55%), followed by MRSA - Methicillin-Resistant Staphylococcus Aureus (108 -13.91%) and Escherichia coli (99 -12.75%). The most common isolates were from respiratory tract (572 isolates -73.71%). High rates of MDR were found for Pseudomonas (73.07%), MRSA (62.03%) and Klebsiella (44.57%). The study revealed an alarming pattern of antibiotic resistance in the majority of ICU isolates from elderly patients (�65 years), which draws attention to the need for judicious use of antibiotics and for careful monitoring of the drug resistance of patients.

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Section: Resultssupporting

confidence: 82%

Antibiotic Resistance Pattern of Bacterial Pathogens in Elderly Patients Admitted in the Intensive Care Unit

Golli¹,

Niţu²,

Bălăşoiu³

et al. 2019

Rev. Chim.

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“…Most patients enrolled in this study received antibiotic therapy prior to various surgeries. It was found that antibiotic prophylaxis was not effective against the isolated pathogenic strains, particularly MRSA, enterococcus and Gram-negative bacillus species, additional prophylactic measures being necessary [5].…”

Section: Resultsmentioning

confidence: 99%

Antibioresistance of Microbial Strains Implicated in the Etiology of Sepsis with Oro-Maxillofacial Portal of Entry

Constantinescu¹,

Plesca²,

Postolache³

et al. 2018

Rev. Chim.

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Methicillin-resistance phenomenon regarding Staphylococcus aureus which is often met as etiologic agent of severe systemic infections with oral-maxillofacial portal of entry imposes the first-line therapeutic schemes readjustment in patients with significant risk factors. Minimum inhibitory concentration (MIC) determination for every isolated S.aureus strain is useful for the antibiotherapy guiding, in order to choose the appropriate antimicrobial substances and to avoid the selection of resistant mutants. There have been studied and tested 9036 bacterial strains isolated from patients hospitalized in the Sf.Spiridon Emergency County Hospital between 2013-2016. Minimum inhibitory concentrations (MIC), MIC 50 and MIC 90 values were determined for the following antibiotics: Penycilline, Erithromycin, Oxacylline, Tetracycline, Gentamycin, Tobramycine, Kanamycin, Ryfampicyn, Trimethoprim-Sulfamethoxazole, Ofloxacine, Ciprofloxacine and Vancomycin.The classification of each identified bacterial strain into sensitive or resistant was accomplished according to the breakpoints recommended by CLSI 2016 (Clinical and Laboratory Standard Institute). We considered intermediately susceptible isolates as being resistant. S.aureus antibioresistance was high to tetracycline, erythromycin and kanamycin, with elevated MIC 90 values (64�g). The rate of resistance to penicillin in the case of S.aureus was 94.7%. The lowest MIC values regarding Pseudomonas aeruginosa were for imipenem, meropenem and colistin and the highest ones for piperacillin-tazobactam, ceftazidime and amikacin. Third generation cephalosporins demonstrated their inefficiency in the staphylococcal infections� treatment as a consequence of an increasing resistance to this category of betalactams. Vancomycin remains a saving in-hospital therapeutic option in the case of MRSA implication, next to teicoplanin and linezolid.

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“…In other studies conducted with the aim of highlighting the MDR strains which caused other types of infections besides BSI, Klebsiella ranks first among isolated microorganisms, 12 , 14 , 15 with the same findings in other researchers’ studies. 16 …”

Section: Discussionmentioning

confidence: 99%

Prevalence of Multidrug-Resistant Pathogens Causing Bloodstream Infections in an Intensive Care Unit

Golli¹,

Cristea²,

Zlatian³

et al. 2022

IDR

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Introduction Bloodstream infections are the most severe infections that cause the highest mortality rate, especially in patients admitted to the intensive care unit (ICU). In this study, we aimed to analyze the distribution, resistance patterns and prevalence of MDR (multidrug-resistant) pathogens isolated in blood samples collected from patients with severe invasive infections hospitalized in the ICU. Methods A retrospective study of bacterial pathogens was performed on 490 patients admitted to the ICU between 2017 and 2020. The resistance patterns were analyzed using Vitek 2 Compact system. Results In total, 617 bacterial isolates were obtained. Four hundred and twenty-seven isolates (69.21%) were Gram positive and 190 isolates (30.79%) were Gram negative bacteria. The most frequently isolated micro-organisms identified in the blood samples for the entire period (2017–2020) were Coagulase-negative staphylococci (CoNS) (318–51.54%), followed by Klebsiella pneumoniae (70–11.34%), Methicillin-Resistant Staphylococcus aureus (MRSA) (58–9.40%), Acinetobacter baumannii (45–7.29%) and Enterococcus faecalis (42–6.80%). The number of Klebsiella pneumoniae strains significantly increased in 2020, compared to the previous year (p < 0.05). The Acinetobacter baumannii prevalence was significantly higher in the age group of 20–64 years (10.89%) and over 65 years (3.53%) (p < 0.001). The difference between the prevalence of CoNS in the elderly (67.84%) and in adults (20–64 years) (52.47%) was also statistically significant (p < 0.001). High rates of MDR were found for Acinetobacter baumannii (97.77%), Pseudomonas aeruginosa (65%), Klebsiella pneumoniae (50%), Enterococcus faecalis (47.61%) and MRSA (46.55%). More than 60% of the Klebsiella pneumoniae strains were found to be resistant to carbapenems. Conclusion The study revealed an alarming prevalence of MDR strains isolated in blood samples of the patients admitted to the ICU, indicating the necessity of consistent application of the measures to control.

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Microarray Technology for the Detection of Antimicrobial Resistance Genes Present in Hospital and Community Acquired Methicillin Resistant S.aureus Strains

Cited by 4 publications

References 14 publications

Antibiotic Resistance Pattern of Bacterial Pathogens in Elderly Patients Admitted in the Intensive Care Unit

Antibiotic Resistance Pattern of Bacterial Pathogens in Elderly Patients Admitted in the Intensive Care Unit

Antibioresistance of Microbial Strains Implicated in the Etiology of Sepsis with Oro-Maxillofacial Portal of Entry

Prevalence of Multidrug-Resistant Pathogens Causing Bloodstream Infections in an Intensive Care Unit

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