Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions

Håti, Armend Gazmeno; Arnfinnsdottir, Nina Bjørk; Østevold, Camilla; Sletmoen, Marit; Etienne, Gianluca; Amstad, Esther; Stokke, Bjørn Torger

doi:10.1039/c6ra23945e

Cited by 10 publications

(5 citation statements)

References 66 publications

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“…Our mask printing source limits features to approximately 10 μm at their highest resolution, thus making it difficult to accommodate single drops with diameters <20 μm in features that allow for one drop per well. These same limitations make the fabrication of other drop arrays, such as floating arrays (Shim et al, 2009; Khorshidi et al, 2014; Håti et al, 2016), anchors (Abbyad et al, 2011), and hydrodynamic drop traps (Shi et al, 2008; Bai et al, 2010; Bithi and Vanapalli, 2010; Sun et al, 2011), unrealistic for small scale microfluidic drops.…”

Section: Discussionmentioning

confidence: 99%

“…The advantage of this method is in its simplicity. Compared to other microfluidic fabrication methods used to store drops on chip, such as glass fabrication (Boitard et al, 2012), pumps and valves to compartmentalize cells (Thorsen et al, 2002; Leung et al, 2012), multilayer PDMS devices with water reservoirs (Dewan et al, 2012; Leung et al, 2012), floating or sunken arrays (Shim et al, 2009; Khorshidi et al, 2014; Håti et al, 2016), or fabricating channels on a microfluidic device to trap single cells (Balaban et al, 2004; Rowat et al, 2009; Wang et al, 2010), the DropSOAC approach simply uses a tight-lidded Petri dish to maintain fluid phase equilibrium in the PDMS surrounding the drops. The enclosed, transportable, and compact DropSOAC capsule allows drops to remain stationary and maintain their size over long hours of time-lapse imaging.…”

Section: Discussionmentioning

confidence: 99%

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DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology

et al. 2019

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The physiological heterogeneity of cells within a microbial population imparts resilience to stresses such as antimicrobial treatments and nutrient limitation. This resilience is partially due to a subpopulation of cells that can survive such stresses and regenerate the community. Microfluidic approaches now provide a means to study microbial physiology and bacterial heterogeneity at the single cell level, improving our ability to isolate and examine these subpopulations. Drop-based microfluidics provides a high-throughput approach to study individual cell physiology within bacterial populations. Using this approach, single cells are isolated from the population and encapsulated in growth medium dispersed in oil using a 15 μm diameter drop making microfluidic device. The drops are arranged as a packed monolayer inside a polydimethylsiloxane (PDMS) microfluidic device. Growth of thousands of individual cells in identical microenvironments can then be imaged using confocal laser scanning microscopy (CLSM). A challenge for this approach has been the maintenance of drop stability during extended time-lapse imaging. In particular, the drops do not maintain their volume over time during incubation in PDMS devices, due to fluid transport into the porous PDMS surroundings. Here, we present a strategy for PDMS device preparation that stabilizes drop position and volume within a drop array on a microfluidic chip for over 20 h. The stability of water-in-oil drops is maintained by soaking the device in a reservoir containing both water and oil in thermodynamic equilibrium. This ensures that phase equilibrium of the drop emulsion fluids within the porous PDMS material is maintained during drop incubation and imaging. We demonstrate the utility of this approach, which we label DropSOAC (Drop Stabilization On A Chip), for time-lapse studies of bacterial growth. We characterize growth of Pseudomonas aeruginosa and its Δhpf mutant derivative during resuscitation and growth following starvation. We demonstrate that growth rate and lag time heterogeneity of hundreds of individual bacterial cells can be determined starting from single isolated cells. The results show that the DropSOAC capsule provides a high-throughput approach toward studies of microbial physiology at the single cell level, and can be used to characterize physiological differences of cells from within a larger population.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology

et al. 2019

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“…Beberapa peneliti sebelumnya telah melakukan pengamatan tentang penerapan prinsip prinsip Good Corporate Governance dalam meningkatkan kinerja karyawan menunjukkan hasil yang tidak konsisten (Hati & Arumrasmy, 2016). Menjelaskan bahwa prinsip independensi dan pertanggungjawaban berpengaruh terhadap kinerja karyawan, sementara prinsip yang lain: transparansi, akuntabilitas dan kewajaran tidak terpengaruh terhadap kinerja karyawan.…”

Section: Pendahuluanunclassified

Analisis Studi Literatur Jurnal Dampak Tata Kelola Perusahaan Terhadap Kebahagiaan Karyawan Dengan Kompensasi Finansial Sebagai Moderasi Menggunakan Metode Bibliometrik

Irfan,

Ismanto,

Sukasi

et al. 2024

COSTING

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Penerapan tata kelola Perusahaan dengan budaya kerja serta kompetensi sangat sering digunakan dalam pelaksanaan proyek konstruksi bangunan industri pertambangan maupun infrastruktur kota dengan berbagai macam metode seiring dengan tuntutan zaman dan teknologi, sehingga perlu dilakukan kajian mengenai dampak penerapan tata kelola perusahaan terhadap kebahagiaan karyawan dengan kompensasi finansial sebagai moderasi. tinjauan literatur bertujuan untuk secara sistematis mendapatkan informasi perkembangan penelitian ilmiah dan menganalisis dampak tata kelola perusahaan terhadap kebahagiaan karyawan yang di moderasi oleh kompensasi finansial sebagai acuan kajian ilmiah saat ini dan masa depan dengan menggunakan teknik tinjauan kuantitatif analisis bibliometrik, data diambil dari database Scopus dengan menggunakan software publish or Perish (PoP) selanjutnya difilter menggunakan software Mendeley dan dianalisis menggunakan software VOSviewer dari 300 artikel, hanya 220 yang terpilih, berdasarkan istilah dan kata yang paling relevan yang akan dianalisis. Hasil analisis menunjukkan bahwa dalam bidang Tata Kelola Perusahaan dan kebahagiaan karyawan yang di moderasi oleh Kompensasi finansial adalah topik yang menjadi perhatian yang diperoleh pada tahun 2014 hingga 2023 menunjukkan bahwa topik kebahagiaan karyawan merupakan penelitian terbaru sebelum masa pandemi. Secara keseluruhan penelitian ini dapat digunakan oleh peneliti sebagai dasar penelitian di masa depan topik yang memerlukan kajian lebih lanjut yaitu green culture serta budaya stempat sehingga menciptakan performance tenaga kerja yang baik di ukur dari tingkat loyalitas kerja dengan menggunakan teknologi terkini. Kata Kunci: Tata Kelola Perusahaan; Kebahagiaan Karyawan; Kompensasi Finansial; Budaya Kerja; Manajemen Konstruksi; Loyalitas Karyawan; Konstruksi Tambang; Biblometric Kabupaten Mimika.

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“…This ion exchange is sufficiently slow to initiate crosslinking of alginate only after drops have been collected, thereby mitigating the risk for microfluidic channels to clog. [19] Drops can also be solidified by physically crosslinking solutes. This can be achieved through solvent extraction, if the solvent has some solubility in the surrounding phase and thus partitions into it before it evaporates from the liquid-air interface.…”

Section: Solid Particles Made From Emulsion Dropsmentioning

confidence: 99%

Microfluidics: A Tool to Control the Size and Composition of Particles

Amstad¹

2017

Chimia

Self Cite

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Particles and capsules are used as containers for active ingredients to delay their degradation and control the location and kinetics of their release. Key to a successful application of these containers is a good control over their size, composition, and the release kinetics of encapsulants. These parameters can be tuned if containers are made from drops of a controlled size and composition; a method that enables formation of drops of a defined size is microfluidics. This review highlights some recent developments in the use of drops made with microfluidics to produce particles and capsules of controlled sizes, compositions, and structures.

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Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions

Abstract: Here, we present two array platforms for small (50–100 μm) cell-containing 3D compartments prepared by droplet-based microfluidics.

Cited by 10 publications

References 66 publications

DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology

DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology

Analisis Studi Literatur Jurnal Dampak Tata Kelola Perusahaan Terhadap Kebahagiaan Karyawan Dengan Kompensasi Finansial Sebagai Moderasi Menggunakan Metode Bibliometrik

Microfluidics: A Tool to Control the Size and Composition of Particles

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