Microassay for rapid measurement of 7-ethoxyresorufin-O-deethylase activity in intact fish hepatocytes

Behrens, Anja; Schirmer, Kristin; Bols, Niels C.; Segner, Helmut

doi:10.1016/s0141-1136(97)00039-1

Cited by 48 publications

(27 citation statements)

References 11 publications

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“…Induction of 7-ethoxyresorufin-O-deethylase was measured in the CYP1A expressing permanent fish liver cell line RTL-W1 (from rainbow trout, O. mykiss) with slight modifications to a method previously published elsewhere (Behrens et al 1998;Gustavsson et al 2004). RTL-W1 cells (Lee et al 1993) were obtained from Drs.…”

Section: Erod Assaymentioning

confidence: 99%

Some heterocyclic aromatic compounds are Ah receptor agonists in the DR-CALUX assay and the EROD assay with RTL-W1 cells

Hinger

Brinkmann

Bluhm

et al. 2011

Environ Sci Pollut Res

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Section: Erod Assaymentioning

confidence: 99%

Some heterocyclic aromatic compounds are Ah receptor agonists in the DR-CALUX assay and the EROD assay with RTL-W1 cells

Hinger

Brinkmann

Bluhm

et al. 2011

Environ Sci Pollut Res

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“…2.9 7-Ethoxyresorufin-O-deethylase assay Induction of EROD was measured in the CYP1A-expressing cell line RTL-W1, with slight modifications to the method described by Behrens et al (1998). RTL-W1 cells were kindly provided by Drs.…”

Section: Gpc2d Assaymentioning

confidence: 99%

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

Wölz

Engwall

Maletz

et al. 2008

Environ Sci Pollut Res

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As a consequence of climate change, flood events will increase in frequency and intensity at least in some regions such as Central Europe. Thus, it is crucial to identify the potential hazard of (re-)mobilized contaminants from reservoirs dislocated via floods and threatening especially aquatic organisms and cattle grazing in flood plains. Since other less persistent compounds seem to be more relevant to explain AhR-mediated activities in flood SPM, nonconventional PAHs and more polar compounds also need to be considered for risk assessment. Effect-directed analysis using broad-range fractionation methods taking into account compounds from polar to nonpolar should be applied for identification of pollutants causing biological effects, thus integrating biological and chemical parameters.

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“…Whereas vertebrate-based in vitro systems have frequently been used for the investigation of aqueous samples (Behrens et al 1998, Brack et al 2000, Castano et al 1994, Ekwall et al 1998, Giesy et al 2002, Hilscherova et al 2000a, b , Hollert et al 2002a, b, Machala et al 2001, Nehls et al 1998, Segner and Segner Braunbeck 1997, there is a significant lack of whole sediment assays (Ensenbach 1998).…”

Section: Jss -J Soilsmentioning

confidence: 99%

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Hollert

Keiter

König

et al. 2003

J Soils & Sediments

201

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (Danio rerio) embryos. Soils and Sediments, http://dx.doi.org/10.1065/jss2003.09.085 Journal ofAccess to the published version may require subscription. Conclusions.A modified protocol of the zebrafish embryo test allows the assessment of sediment toxicity in both aqueous extracts and native sediments. The isolated investigation of pore waters may result in a clear-cut underestimation of the bioavailability of lipophilic particle-bound substances (as determined by native sediments). Recommendations and Perspectives.The zebrafish embryo test with native (whole, solid phase) sediments appears very promising for the evaluation of the bioavailable fraction of lipophilic particle-bound substances and can therefore be recommended for the evaluation of vertebrate toxicity in tiered sediment test strategies and dredging directives such as the HABAB-WSV. Whereas acetone extracts may be tested as a rough estimation of embryotoxicity, native sediment samples will provide a more comprehensive and realistic insight into the bioavailable hazard potential. (2) to compare the hazard potential of solid phase sediments to the effects of corresponding pore waters and organic extracts in order to characterize the bioavailability of the particle-bound pollutants; and (3) to relatively evaluate the embryotoxic effects of sediments from the catchment areas of the rivers Rhine, Neckar and Danube. Keywords Methods (or Main Features).To investigate the toxicity of sediment samples on vertebrates, the standard embryo toxicity test with the zebrafish (Danio rerio; Hamilton-Buchanan 1922) according to DIN 38415-6 was modified with respect to exposure scheme and toxicological endpoints. Sediments from the catchment area of the Neckar River were assessed using pore waters, acetonic extracts and native sediments in order to get inside into the potential bioavailability of particle-bound pollutants. A comprehensive test protocol for the investigation of native sediments in the embryo toxicity test with the zebrafish is presented.Results and Discussion. The fish embryo assay with Danio rerio can be carried out with both aqueous and organic sediment extracts as well as native (whole, solid phase) sediment samples. Elongation of exposure time from 48 to up to 196 h significantly increased the mortality. Using the fish egg assay with native sediments, a broad range of embryotoxic effects could be elucidated, including clear-cut dose-response curves for the embryotoxic effects of contaminated sediments; in contrast, absence of embryotoxic effects could be demonstrated even for the highest test concentrations of unpolluted sediments. With native sediments, embryotoxicity was clearly higher than with corresponding pore waters, thus corroborating the view that -at least for fish eggs -the bioavailability of particle-bound lipophilic substances in native sediments is higher than generally assumed. The relative ranking of sediment toxicity was identical using both nati...

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Microassay for rapid measurement of 7-ethoxyresorufin-O-deethylase activity in intact fish hepatocytes

Cited by 48 publications

References 11 publications

Some heterocyclic aromatic compounds are Ah receptor agonists in the DR-CALUX assay and the EROD assay with RTL-W1 cells

Some heterocyclic aromatic compounds are Ah receptor agonists in the DR-CALUX assay and the EROD assay with RTL-W1 cells

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

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