1996
DOI: 10.1093/clinchem/42.11.1805
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Microassay of amiodarone and desethylamiodarone in serum by capillary electrophoresis with head-column field-amplified sample stacking

Abstract: Binary-system capillary electrophoresis (CE) with head-column field-amplified sample stacking permits determination of amiodarone and desethylamiodarone in 20-microL serum samples. The assay is characterized by a detection limit for both compounds of 80 nmol/L and by excellent linear response over the recommended therapeutic range for amiodarone (1.5-4 mumol/L). Intra- and interday reproducibilities (CVs) between 3% and 6% and run times of approximately 10 min are comparable with those for conventional HPLC. B… Show more

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Cited by 58 publications
(15 citation statements)
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“…Head‐column FASS is based upon the presence of a short zone of low conductivity (mostly water is used and the zone is referred to as water plug) at the capillary inlet end across which an electric field much larger than that employed in normal CE is established and which permits charged analytes to be injected at high velocity. During this electrokinetic injection process, analytes are stacked at the interface between the low‐conductivity zone and the running buffer . In this paper, enantioselective CE with head‐column FASS for analysis of threo ‐methylphenidate in extracts of oral fluid is reported and applied to the determination of these enantiomers in oral fluid of a patient who was treated with different formulations of racemic threo ‐methylphenidate.…”
Section: Introductionsupporting
confidence: 91%
See 1 more Smart Citation
“…Head‐column FASS is based upon the presence of a short zone of low conductivity (mostly water is used and the zone is referred to as water plug) at the capillary inlet end across which an electric field much larger than that employed in normal CE is established and which permits charged analytes to be injected at high velocity. During this electrokinetic injection process, analytes are stacked at the interface between the low‐conductivity zone and the running buffer . In this paper, enantioselective CE with head‐column FASS for analysis of threo ‐methylphenidate in extracts of oral fluid is reported and applied to the determination of these enantiomers in oral fluid of a patient who was treated with different formulations of racemic threo ‐methylphenidate.…”
Section: Introductionsupporting
confidence: 91%
“…In the assay, threo ‐methylphenidate enantiomers are determined after liquid/liquid extraction at alkaline pH followed by electrokinetic injection from a slightly acidified low‐conductivity extract across a short water plug placed at the head of the capillary. This effect provides the required nanogram per milliliter sensitivity for this drug . S ‐ketamine (p K a : 7.5 ; log P : 3.1 ; for chemical structure see Fig.…”
Section: Resultsmentioning
confidence: 77%
“…Efficiency of electrokinetic injection of cations is dependent on sample composition, particularly conductivity and pH, properties which should both be low [, ]. Thus, addition of 45.5, 90.9, 228, and 455 μM of phosphoric acid to the sample was assessed for the analytes of interest using the configuration with a 50 mM plug buffer concentration, a 1 psi 20 s plug, and analyte injection at 6 kV for 15 s. Small amounts of phosphoric acid provided increased sensitivity.…”
Section: Resultsmentioning
confidence: 99%
“…Many of the applied methods include electrokinetic sample injection, a process that requires proper control of injection parameters in order to achieve acceptable quantitative repeatability . For analysis of drugs and metabolites in microliter amounts of body fluids, the combination of electrokinetic injection from extracts with low conductivity and field‐amplified sample stacking at the capillary tip provides the basis for ppb (ng/mL) detection limits and robust assays .…”
Section: Introductionmentioning
confidence: 99%
“…Nevertheless, few works have been found with accuracy studies. A validated assay for the determination of the anti-arrhythmic amiodaron and its main metabolites in human serum has been described [5]. Authors say that because of the variations in peak size obtained in head-column field-amplified sample stacking (FASS) and in extraction, typical intra-and interday imprecisions observed with external calibration were 10-20% and this is not to be recommended.…”
Section: Introductionmentioning
confidence: 99%