Microbacterium suaedae sp. nov., isolated from Suaeda aralocaspica

Zhu, Zhi-Nan; Li, Yanru; Li, Yuqian; Xiao, Min; Han, Ming-Xian; Wadaan, Mohammed A. M.; Hozzein, Wael N.; An, Deng-Di; Li, Wenjun

doi:10.1099/ijsem.0.003162

“…As far as we know, the first isolation, description and taxonomic characterization of a novel species from S. aralocaspica was by Zhu et al [8]. In recent years, some novel species isolated from S. aralocaspica have also been reported [8][9][10][11]. Here we report the characterization of strain YZJH907-2 T , which was isolated from the stem of the halophyte and is the type strain of a novel species in the genus Bacillus.…”

mentioning

confidence: 78%

“…As far as we know, the first isolation, description and taxonomic characterization of a novel species from S. aralocaspica was by Zhu et al [8]. In recent years, some novel species isolated from S. aralocaspica have also been reported [8][9][10][11].…”

mentioning

confidence: 99%

Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China

Lei

¹

,

Ma

²

,

Li

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, designated YZJH907-2T, was isolated from the stem of Suaeda aralocaspica, collected from the southern edge of the Gurbantunggut desert, Xinjiang, PR China. Cells of strain YZJH907-2T were Gram-stain-positive, aerobic and rod-shaped. They formed white or colourless circular colonies with smooth convex surfaces. Strain YZJH907-2T grew at 4–50 °C (optimum, 28–30 °C), pH 7.0–10.0 (optimum, pH 8.0–9.0) and with 0–10 % (w/v) NaCl (optimum, 3–7 %). The genomic DNA G+C content of strain YZJH907-2T was 38.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the strain was most closely related to Bacillus alcalophilus DSM 485T (97.37 %), Bacillus kiskunsagensis B16-24T (96.87 %) and Bacillus bogoriensis LBB3T (96.71 %). Average nucleotide identity values between YZJH907-2T and B. alcalophilus DSM 485Tand B. bogoriensis LBB3T were 69.2 and 69.0 %, respectively. Digital DNA–DNA hybridization values of YZJH907-2T with B. alcalophilus DSM 485T and B. bogoriensis LBB3T were 19.6 and 20.4 %, respectively. The cell wall of strain YZJH907-2T contained meso-diaminopimelic acid, and the major and secondary isoprenoid quinones were MK-7 and MK-5, respectively. Results of fatty acids showed that anteiso-C15 : 0, iso-C15 : 0 and C16 : 0 were the predominant cellular fatty acids. Two-dimensional thin-layer chromatography analysis indicated that the polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and two unidentified glycolipids. Based on the genomic, phylogenetic and phenotypic analyses, strain YZJH907-2T represented a novel species of the genus Bacillus , and thus the name Bacillus suaedae sp. nov. is proposed. The type strain is YZJH907-2T (=CGMCC 1.18763T=KCTC 43335T).

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“…Growth at different temperatures (4,12,20,25,28,30,37,40, 42, 45 and 50 °C) and pH values (6.0-11.0, at intervals of 1.0 pH unit) were tested on TSB medium. NaCl tolerance (1-15 %, w/v, at intervals of 1.0 % unit) was tested using R2A agar as a basal medium.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…This research was supported by the National Natural Science Foundation of China (Grant No. U1703236), the United Foundation of Ocean [28]. †Data from Shivaji et al [29].…”

Section: Funding Informationmentioning

confidence: 99%

Microbacterium karelineae sp. nov. isolated from a halophyte plant in the Taklamakan desert

Zhu

¹

,

Yang

²

,

Luo

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain TRM 80801T grew at 4–50 °C, with optimum growth at 28 °C, and grew at pH 6.0–11.0 and 1–15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively. The DNA G+C content of TRM 80801T is 69.38 mol%. The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine. The acyl type of the peptidoglycan was glycolyl. Whole-cell sugars were ribose, mannose, glucose, rhamnose and galactose. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The predominant menaquinones were MK-10, MK-11 and MK-12. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol. The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %. On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium , for which the name Microbacterium karelineae sp. nov. is proposed. The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).

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“…In addition, herein, we found that the endophytic fungal diversity distinctively contrasted between the delta and the tidal flat areas. Furthermore, from the perspective of securing biological resources, a variety of novel species of endophytic microorganisms were reported in halophytes that are thriving in coastal ecotones [45][46][47][48]. This may represent the existence of a new microbial resource that has evolved and adapted to the particular selective pressures in the coastal ecotone zone, where changes are always extreme.…”

Section: Endophytic Distribution and Diversity Within Salt Marshes In...mentioning

confidence: 99%

Endophytic Fungi of Emersed Halophytes in River Deltas and Tidal Flats of the Korean Ramsar Wetlands

Park

¹

,

Hong

²

,

You

³

et al. 2021

JMSE

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This study aimed to obtain information on the diversity and distribution of the endophytic fungi in Ramsar wetlands. Vast salt marshes in Suncheon Bay, Korea, are formed by two types of ecotones (tidal flats and deltas) that are supported by the emersed halophytes Phragmites australis and Suaeda japonica. Overall, 324 endophytes were isolated from P. australis (six sampling points in the delta and five in the tidal flats) and S. japonica (six in tidal flats). Margalef’s, Menhinick’s, Shannon’s, and Simpson’s diversity indices significantly varied among the ecotones. In particular, higher variance in diversity value and unevenness was observed in the delta marsh compared with the tidal flat marsh. Further, morphological diversity in the delta salt marsh was 1.8 times higher than that of the tidal flat. Comprising several dominant genera (Aspergillus, Cladosporium, and Penicillium), Epicoccum, Paraconiothyrium, Septoriella, and Talaromyces were widely distributed regardless of the aquatic conditions or halophyte species. This study highlights the distinct distribution and diversity of marine endophytes in various ecotones that can physically protect the coastal areas. This data is of much value to secure a national culture collection for future restoration of the coastal wetlands and their ecosystems.

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Microbacterium suaedae sp. nov., isolated from Suaeda aralocaspica

Cited by 11 publications

References 33 publications

Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China

Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China

Microbacterium karelineae sp. nov. isolated from a halophyte plant in the Taklamakan desert

Endophytic Fungi of Emersed Halophytes in River Deltas and Tidal Flats of the Korean Ramsar Wetlands

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