Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells

Jeynes, Jonathan C. G.; Bailey, Michael; Coley, Helen M.; Kirkby, K.J.; Jeynes, C.

doi:10.1016/j.nimb.2010.02.042

Cited by 13 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data indicate that copper transporters might contribute to platinum drug resistance; however, we did not identify a significant correlation between the copper concentration and the effect of chemotherapy in multivariate analysis; thus, further investigation is needed. In addition, it was reported that the distribution of Zn was inversely correlated with that of platinum in ovarian and lung cancer cells, but we observed no such correlation.…”

Section: Discussioncontrasting

confidence: 90%

“…Although the platinum concentration of tumor epithelium was not significantly correlated with clinical or histological response, the higher concentration of accumulated platinum in the CC3‐positive degenerated area than in the CC3‐negative viable tumor epithelium in our study could support this notion. These findings suggest that l ‐OHP‐based chemotherapy was effective in patients when the drugs were sufficiently delivered to cancer cells, as reported previously . In their studies using cell lines or bulk tissues, it is difficult to investigate the effect of the tumor stroma, which constitutes a distinctive microenvironment, thus further study investigating the in situ accumulation of platinum in the tumor epithelium and stroma as well as chronological change of its distribution after administration of chemotherapeutic agent is mandatory.…”

Section: Discussionsupporting

confidence: 60%

“…7 Tanaka et al 8 reported that cisplatin-sensitive esophageal squamous cancer cells accumulated more platinum than resistant cells in vitro. Similarly, Jeynes et al 9 reported platinum accumulation in sensitive ovarian cancer cells. Moreover, Kim et al 10 identified a positive correlation between the tissue platinum concentration and response to platinum-based neoadjuvant chemotherapy in advanced nonsmall cell lung cancer.…”

Section: Introductionmentioning

confidence: 84%

“…These findings suggest that L-OHPbased chemotherapy was effective in patients when the drugs were sufficiently delivered to cancer cells, as reported previously. [8][9][10] In their studies using cell lines or bulk tissues, it is difficult to investigate the effect of the tumor stroma, which constitutes a distinctive microenvironment, thus further study investigating the in situ accumulation of platinum in the tumor epithelium and stroma as well as chronological change of its distribution after administration of chemotherapeutic agent is mandatory.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative evaluation of the intratumoral distribution of platinum in oxaliplatin‐treated rectal cancer: In situ visualization of platinum via synchrotron radiation X‐ray fluorescence spectrometry

Koba

Fujita

Nishibori

et al. 2019

Intl Journal of Cancer

View full text Add to dashboard Cite

Oxaliplatin (l‐OHP), a platinum‐based drug, is a key chemotherapeutic agent for colorectal cancer (CRC), but drug resistance and toxic effects have been major limitations of its use. Synchrotron radiation X‐ray fluorescence spectrometry (SR‐XRF) is a rapid, nondestructive technique for monitoring the distribution of metals and trace elements in cells or tissue samples. We applied SR‐XRF to visualize the distribution of platinum and other elements in 30 rectal cancer specimens resected from patients who received l‐OHP‐based preoperative chemotherapy and quantified platinum concentration in the tumor epithelium and stroma, respectively, using calibration curves. The platinum concentration in rectal cancer tissue ranged 2.85–11.44 ppm, and the detection limit of platinum was 1.848 ppm. In the tumor epithelium, the platinum concentration was significantly higher in areas of degeneration caused by chemotherapy than in nondegenerated area (p < 0.001). Conversely, in the tumor stroma, the platinum concentration was significantly higher in patients with limited therapeutic responses than in those with strong therapeutic responses (p < 0.001). Furthermore, multivariate analysis illustrated that higher platinum concentration in the tumor stroma was an independent predictive factor of limited histologic response (odds ratio; 19.99, 95% confidence interval; 2.04–196.37, p = 0.013). This is the first study to visualize and quantify the distribution of platinum in human cancer tissues using SR‐XRF. These results suggest that SR‐XRF analysis may contribute to predicting the therapeutic effect of l‐OHP‐based chemotherapy by quantifying the distribution of platinum.

show abstract

Section: Discussioncontrasting

confidence: 90%

Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative evaluation of the intratumoral distribution of platinum in oxaliplatin‐treated rectal cancer: In situ visualization of platinum via synchrotron radiation X‐ray fluorescence spectrometry

Koba

Fujita

Nishibori

et al. 2019

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Because the energy loss and irradiation effect of hundred-keV ions on the surface are considerably stronger than those of keV and MeV ions, microsized or nanosized ion beams with hundred-keV energies have broad applications, ranging from microsurgery in living cells 20 21 22 to the controlled nanopatterning 23 24 25 of material surfaces, e.g., forming regular nanopore arrays on graphene 26 27 .…”

mentioning

confidence: 99%

Transmission of Hundred-keV Protons through Insulating Nanocapillaries: Charge-patch-assisted Specular Reflections

Wang

Shao

Song

et al. 2015

Sci Rep

View full text Add to dashboard Cite

In this work, we measured the time evolution of the transmission features of 10–100 keV protons transmitted through nanocapillaries in a polycarbonate (PC) membrane. After reaching equilibrium, transmitted particles with an incident energy of 100 keV were located around the direction along the incident beam but not along the capillary axis, indicating that the transport mechanism of the 100 keV ion was distinct from that of keV-energy ions. The simulation results indicated that charge-patch-assisted collective scatterings on the surface are the main transport mechanism for the hundred-keV ions in nanocapillaries. This scenario fills in the gap in the previous understanding of ion transmission in nanocapillaries from keV to MeV energies.

show abstract

Particle‐InducedX‐Ray Emission

Reis¹,

Campbell²

2012

Characterization of Materials

View full text Add to dashboard Cite

Particle‐Induced X‐Ray Emission (PIXE) is an analytical technique established in the decade of 1970s, which makes use of MeV ion beams (mainly proton beams) to induce x‐ray emission from materials. It is used both in macro mode, where beam spots are of the order of millimeters, and in micro mode, where ion beams are focused to micrometer size (or even below this, since 100 nm spots are already available for PIXE). It has major advantages for the analysis of sensitive and/or complex samples because it is essentially a non‐destructive technique. For example, some laboratories are equipped with facilities where the beam is extracted into the atmosphere, allowing the analysis of paintings and other cultural heritage samples. Recent developments also make possible the analysis of samples that are not homogeneous but composed of different layers. In this case, PIXE has large advantages relative to electron microprobe analysis (EPMA) because the particle beam scattering is very small, such that the micro‐beam remains focused along several micrometers into the sample. Finally, the use of x‐ray detection systems having high resolution (resolving power of the order of 100 or more) is presently moving from pure fundamental work towards applications as more complex samples need to be analyzed.

show abstract

Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells

Cited by 13 publications

References 16 publications

Quantitative evaluation of the intratumoral distribution of platinum in oxaliplatin‐treated rectal cancer: In situ visualization of platinum via synchrotron radiation X‐ray fluorescence spectrometry

Quantitative evaluation of the intratumoral distribution of platinum in oxaliplatin‐treated rectal cancer: In situ visualization of platinum via synchrotron radiation X‐ray fluorescence spectrometry

Transmission of Hundred-keV Protons through Insulating Nanocapillaries: Charge-patch-assisted Specular Reflections

Particle‐InducedX‐Ray Emission

Contact Info

Product

Resources

About