Microbial activities in deep subsurface environments

Phelps, Tommy J.; Raione, E. G.; White, David C.; Fliermans, C.B.

doi:10.1080/01490458909377851

Cited by 129 publications

(60 citation statements)

References 20 publications

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“…Nutrients, pollutants or electron donors reach the microorganisms and the Terminal Electron Accepting Processes can take place because of this porosity (Chapelle, 1993 andFredrickson et al, 1997). In fact, several studies show a greater bacterial abundance and activity in sandy rather than clayey sediments Hicks and Fredrickson, 1989;Chapelle and Lovley, 1990and Phelps et al, 1989.…”

Section: Microbial Heterogeneitymentioning

confidence: 99%

Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history

Puigserver

Carmona

Cortés

et al. 2013

Journal of Contaminant Hydrology

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Section: Microbial Heterogeneitymentioning

confidence: 99%

Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history

Puigserver

Carmona

Cortés

et al. 2013

Journal of Contaminant Hydrology

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“…After 20 sec of mixing, samples were filtered (using a 0.2 pm-pore-diameter PVDF filter from Whatman, Clifton, New Jersey) and the concentration of Fe (11) in the filtrate was measured at A562. The anaerobic water/ferrozine extraction method used here underestimated the amount of Fe (11) produced, because it did not extract solid Fe (11) compounds such as FeCO3; consequently, the mass balances of iron were typically < 50%.…”

Section: Bacterial Strain Selection and Growthmentioning

confidence: 99%

“…No significant reduction (c 3%) of these metals was detected at 75OC in pure anaerobic water (data not shown). The initial Fe (11) concentration was about 0.3 mh4 when Fe (111)-citrate (final concentration of 14 mM) was added. This Fe (11) probably resulted from reduction of Fe (111)-citrate during autoclaving of the stock solution.…”

Section: @I and Cr (Vi) Reductionmentioning

confidence: 99%

“…Experiments for abiotic and biotic metal reduction were performed using 15-mL pressure tubes (from Bellco Glass Inc., Vineland, New Jersey) that contained a medium having the following ingredients (g/L): NaCl, 5-70; MgC12 -6H20,0.2; CaC12 * 2H20,O.l; W C l , 1; 3-(N-morpho1ino)-propanesulfonic acid, 0.1 ; NaHCO3, 2.5; yeast extract, 1.0-1.5; peptone, 1.0; and trace mineral and vitamin solutions (11). The medium was prepared anaerobically under a nitrogen gas atmosphere.…”

Section: Medium Preparation For Abiotic and Biotic Metal Reductionmentioning

confidence: 99%

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Enhancement of Fe(III), Co(III), and Cr(VI) reduction at elevated temperatures and by a thermophilic bacterium

Zhang

Shi

Logan

et al. 1996

Appl Biochem Biotechnol

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An unusual thermophilic bacterium has been isolated from deep subsurface sediments and tested for its ability to enhance Fe (m), Co (m>, and Cr (Vi) reduction. Without the * bacterium, abiotic metal reduction was insignrfcant at temperams below 45OC but became a major process at 75OC. Addition of the bacterium enhanced the reduction of these metals up to fourfold. This study demonstrates abiotic and biotic metal reduction under organicrich thermic conditions and suggests that thermally and/or biologically enhanced metal reduction may provide an alternative for remediating metal contamination.

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“…Cl 2 , 10 mL/L of trace mineral solution, 1.0 ml/L of 10X vitamin solution, 0.1 g/L of MOPS, 10 mM of NaSO 4 , 40 mM of CH 3 COONa, and 1.0 ml/L of 0.1 percent resazurin solution; Phelps et al, 1989). The redox state was adjusted by addition of 2 percent Cysteine-HCl as needed.…”

Section: Generation Of Reduced Rifle Aquifer Sedimentmentioning

confidence: 99%

In‐well sediment incubators to evaluate microbial community stability and dynamics following bioimmobilization of uranium

Baldwin

Peacock²,

Gan

et al. 2009

Remediation Journal

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An in-well sediment incubator (ISI) was developed to investigate the stability and dynamics of sediment-associated microbial communities to prevailing subsurface oxidizing or reducing conditions. Herein we describe the use of these devices at the Old Rifle Uranium Mill Tailings Remedial Action (UMTRA) site. During a seven-month period in which oxidized Rifle Aquifer background sediment (RABS) were deployed in previously biostimulated wells under ironreducing conditions, cell densities of known iron-reducing bacteria, including Geobacteraceae, increased significantly, showing the microbial community response to local subsurface conditions. Phospholipid fatty acid (PLFA) profiles of RABS following in situ deployment were strikingly similar to those of adjacent sediment cores, suggesting ISI results could be extrapolated to the native material of the test plots. Results for ISI deployment with laboratory-reduced sediments showed only slight changes in community composition and pointed toward the ability of the ISI to monitor microbial community stability and response to subsurface conditions. INTRODUCTIONEnhanced bioremediation, whether for petroleum hydrocarbons, chlorinated solvents, or radionuclides, frequently involves subsurface injection of an amendment (electron acceptor or donor) to stimulate microbial activity. Following injection, temporal groundwater monitoring programs are commonly instituted to track contaminant concentrations, geochemical parameters, and changes in microbial community composition. Although groundwater monitoring can provide valuable insight into biological processes, important microbial populations, and contaminant transformations may be strongly associated with the subsurface matrix (Bekins et al., 1999;Hazen et al., 1991;Thomas et al., 1998) as opposed to the planktonic communities. Collection of sediment cores allows interrogation of sediment-associated transformations. However, repeated drilling events are expensive and not always practical, especially in deeper aquifer systems. To combat this problem, a number of researchers have employed in situ microcosms in which native sediment or a sediment surrogate is deployed and recovered from existing monitoring wells (Bennett et al., 2000;Hendricks et al., 2005;Reardon et al., 2004). The in situ microcosm approach, however, is potentially limited by the extent to which the results relate to the native sediment and the aquifer as a whole (Bennett et al., 2000). Therefore, the cornerstone of the in-well sediment incubator approach is that analysis of recovered microcosms provides interpretable results consistent with sediment core samples. In aerobic aquifers typical of many Department of Energy (DOE) legacy waste sites, uranium is present in the oxidized U(VI) form, which is more soluble and, thus, more mobile (Anderson et al., 2003;Istok et al., 2004;Wall & Krumholz, 2006). A wide variety of dissimilatory metal-reducing bacteria, most notably of the genera Geobacter and Shewanella, have been shown to enzymatically reduce U(VI) to less ...

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Microbial activities in deep subsurface environments

Cited by 129 publications

References 20 publications

Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history

Subsoil heterogeneities controlling porewater contaminant mass and microbial diversity at a site with a complex pollution history

Enhancement of Fe(III), Co(III), and Cr(VI) reduction at elevated temperatures and by a thermophilic bacterium

In‐well sediment incubators to evaluate microbial community stability and dynamics following bioimmobilization of uranium

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