Microbial activity and phylogeny in ice cores retrieved from Lake Paula, a newly detected freshwater lake in Antarctica

Sattler, Birgit; Waldhuber, Sebastian; Fischer, Hanno; Semmler, Hans; Sipiera, P. P.; Psenner, Roland

doi:10.1117/12.564554

Cited by 4 publications

(5 citation statements)

References 2 publications

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“…If infall material incorporated into the near-surface ice in the form of cryoconite sediments is relatively rich in organic material (Anesio et al, 2009) and microbial communities that include photosynthetic cyanobacteria (Priscu et al, 1998;Psenner et al, 1999;Sattler et al, 2004), then the availability of sunlight to provide both the heat energy necessary to melt the ice surrounding the assemblage and the energy for photosynthesis would confer a significant selective advantage on embedded cyanobacteria. Subsequent growth in the short Antarctic growing season should produce cyanobacteria-dominated assemblages that could be detected within the first few centimeters of the surface with fluorescence emission following excitation at 532 nm.…”

Section: Hypothesis and Objectivesmentioning

confidence: 99%

“…T he ices of Earth's polar regions and high mountains, once seen as sterile, harsh environments too poor in nutrients and liquid water to sustain life, are now known to harbor rich, complex microbial communities in the ice ecosystems of alpine and polar lakes, sea ice, glacier ice, and even the ice of supercooled cloud droplets (Priscu et al, 1998;Psenner and Sattler, 1998;Psenner et al, 1999;Sattler et al, 2001Sattler et al, , 2004Priscu and Christner, 2004). Central to the establishment of microbial communities in polar ice is the eolian transport and deposition of dust particles that contain both humic material and microbial life, including photosynthetic cyanobacteria (Psenner, 1999;Porazinska et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Laser-Induced Fluorescence Emission (L.I.F.E.):In SituNondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes

Storrie-Lombardi¹,

Sattler

2009

Astrobiology

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Laser-induced fluorescence emission (L.I.F.E.) images were obtained in situ following 532 nm excitation of cryoconite assemblages in the ice covers of annual and perennially frozen Antarctic lakes during the 2008 Tawani International Expedition to Schirmacher Oasis and Lake Untersee in Dronning Maud Land, Antarctica. Laser targeting of a single millimeter-scale cryoconite results in multiple neighboring excitation events secondary to ice/air interface reflection and refraction in the bubbles surrounding the primary target. Laser excitation at 532 nm of cyanobacteria-dominated assemblages produced red and infrared autofluorescence activity attributed to the presence of phycoerythrin photosynthetic pigments. The method avoids destruction of individual target organisms and does not require the disruption of either the structure of the microbial community or the surrounding ice matrix. L.I.F.E. survey strategies described may be of interest for orbital monitoring of photosynthetic primary productivity in polar and alpine glaciers, ice sheets, snow, and lake ice of Earth's cryosphere. The findings open up the possibility of searching from either a rover or from orbit for signs of life in the polar regions of Mars and the frozen regions of exoplanets in neighboring star systems.

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Section: Hypothesis and Objectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laser-Induced Fluorescence Emission (L.I.F.E.):In SituNondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes

Storrie-Lombardi¹,

Sattler

2009

Astrobiology

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“…The lack of light during the freezing period reduced the photosynthesis of algae, resulting in less organic matter in water and impacting the microbial community (Cross Sattler et al 2004). Except for cyanobacteria affected by specific environmental factors, the microbial community structure in the lakes in the cold-arid regions of northern China during the freezing period was similar to that in the Antarctic, with Actinomyces, Bacteroides, Proteus, and other anaerobic bacteria as the dominant species .…”

Section: Effects Of Lake Microorganisms On Dom Components During Free...mentioning

confidence: 99%

Eutrophication in cold-arid lakes: molecular characteristics and transformation mechanism of DOM under microbial action at the ice-water interface

Wang,

Feng,

Liu

et al. 2024

Carbon Res.

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During freezing periods, nutrients (carbon and organic matter, etc.) are enriched in the water and sediment of lakes in cold-arid regions, leading to potential algal bloom outbreaks and other health risks to the ecosystem. Particularly, dissolved organic matter (DOM) is a critical component of the nutrients and plays an important role in the global carbon cycle. However, the mechanisms of DOM transfer between ice and water remain unclear. This study analyzed the influence of microbial community on DOM composition using 16 s RNA, 3DEEM, and FT-ICR MS in Daihai Lake and Wuliangsuhai Lake in the Yellow River Basin, China. According to the spectral analysis, the content of endogenous organic matter, such as humus, accounted for 40% of the total DOM in water, while the content of tryptophan and tyrosine accounted for 80% of the total DOM in ice. The results of mass spectrometry showed that lignin was the main component, and the content of organic matter in the ice was less than that in the water. Molecular structures of seven DOM coexisting in the lake ice and water were elucidated with adapted Kendrick-analogous network visualization, which clearly illustrates that long-chain DOM molecules are derived from small molecules, while other heteroatoms are complexed with the side groups. The positive correlations between CHO, CHNO, CHOS, CHOS and Actinomyces indicate that DOM actively interacted with the microbial community. 44% of CHO compounds have the same molecular formula in water, the content of CHOS in the water of the two lakes was closed to 7% higher than that in the ice. Meanwhile, DOM dynamically migrate between ice and water via interstitial water because of the solubility changes under microbial transformation, which has been proved by the decrease in the contents of the humus and tryptophan-like substances in the ice from the bottom to the surface and lower contents of carbohydrate and unsaturated aromatic hydrocarbon in the water than the ice. This study helps to predict the composition and structure of DOM during the migration in lakes and provides a scientific basis for environmental remediation with high concentration of carbon.

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“…The lake-ice covers and glaciers of Earth’s cryosphere are home to complex microbial communities (Priscu and others, 1998; Psenner and Sattler, 1998; Psenner and others, 1999; Priscu and Christner, 2004; Sattler and others, 2004). Aeolian transport deposits dust particles onto these frozen surfaces containing humic material and microbial life, including photosynthetic cyanobacteria (Psenner, 1999; Sattler and others, 2001; Porazinska and others, 2004; Foreman and others, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Signal analysis of the LIFE images obtained from the ice cover of the perennially frozen Lake Untersee, Dronning Maud Land, East Antarctica, revealed no significant decrease in photosynthetic biomass in the first meter of the clear ice (Storrie-Lombardi and Sattler, 2009). If lake ice cryoconites are relatively rich in organic material, as in supraglacial environments (Anesio and others, 2009), and the associated microbial communities include photosynthetic cyanobacteria (Priscu and others, 1998; Psenner and others, 1999; Sattler and others, 2004), then the primary limiting factor for biomass will be the translucency of the ice and the availability of sunlight to provide both the energy necessary to melt the ice surrounding the assemblage and the energy for photosynthesis. Microbial communities dominated by cyanobacteria (Taton and others, 2003) conduct photosynthesis inside the ice cover during a few weeks in the austral summer (Fritsen and Priscu, 1998; Priscu and others, 2005).…”

Section: Introductionmentioning

confidence: 99%

Laser-induced fluorescence emission (LIFE) from Lake Fryxell (Antarctica) cryoconites

et al. 2010

Self Cite

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Laser-induced fluorescence emission (LIFE) images were obtained in situ from a 27 cm long ice core at Lake Fryxell, Antarctica. The excitation was accomplished with a simple 532 nm green laser pen light, and the fluorescence images were captured with a small compact digital camera. The targets for the experiment were mm-scale cryoconite assemblages found in the ice covers of this perennially frozen Antarctic lake. The fluorescence response originates from photo-pigments in cyanobacteria-dominated cryoconite assemblages with phycoerythrin (PE) exhibiting the optimal target cross section. This inexpensive, low-mass, low-energy method avoids manipulation of the in situ habitat and individual target organisms and does not disturb the microbial community or the surrounding ice matrix. We establish the correlation between fluorescence intensity and PE concentration. We show that cryoconite fluorescence response does not appear to decrease with depth in the ice cover, in agreement with similar findings at Lake Untersee, a perennially ice-covered lake in Dronning Maud Land, Antarctica. Optical reflection and refraction events at the air/ice interface can complicate quantitative estimates of total pigment concentrations. Laser targeting of a single mm-scale cryoconite can result in multiple neighboring excitation events secondary to reflection and refraction phenomena in the multiple air/ice interface of the bubbles surrounding the primary target.

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Microbial activity and phylogeny in ice cores retrieved from Lake Paula, a newly detected freshwater lake in Antarctica

Cited by 4 publications

References 2 publications

Laser-Induced Fluorescence Emission (L.I.F.E.):In SituNondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes

Laser-Induced Fluorescence Emission (L.I.F.E.):In SituNondestructive Detection of Microbial Life in the Ice Covers of Antarctic Lakes

Eutrophication in cold-arid lakes: molecular characteristics and transformation mechanism of DOM under microbial action at the ice-water interface

Laser-induced fluorescence emission (LIFE) from Lake Fryxell (Antarctica) cryoconites

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