Microbial Analysis of Endodontic Infections in Root-filled Teeth with Apical Periodontitis before and after Irrigation Using Pyrosequencing

Zandi, Homan; Kristoffersen, Anne Karin; Ørstavik, Dag; Rôças, Isabela N.; Siqueira, José F.; Enersen, Morten

doi:10.1016/j.joen.2017.11.019

Cited by 72 publications

(73 citation statements)

References 37 publications

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“…reported a series of abundant genera among the 152 OTUs identified, such as Fusobacterium (12.7%), Streptococcus (9.8%), Actinomyces (8.2%), Desulfobulbus (5.2%), Fretibacterium (3.6%), Treponema (2.3%), or Prevotella (2%) along with Enterococcus . Although Enterococcus was present in only two of ten samples in this study, it represented the most abundant genera (13.9%) . These results concur with Bouillaguet et al.…”

Section: Resultssupporting

confidence: 92%

“…Although NGS results somewhat confirm that E. faecalis is preferentially detected in SAPs, the species was detected in significantly lower frequencies; four of 10 SAPs (40%) in Siqueira et al., two of ten SAPs (20%) in Zandi et al., and seven of 22 SAPs (32%) in Bouillaguet et al. (Table ) . Interestingly, despite lower prevalence values, the species could reach fairly high average abundances; 14% in Zandi et al.…”

Section: Discussionmentioning

confidence: 89%

“…In addition to NECs, negative controls serve to assess the contamination background specific to the sampling site . Herein, 12 of 18 studies have included negative controls mainly taken from endodontic access cavities or external root surfaces after disinfection, to verify the relative “sterility” of the site prior to sampling . Among these 12 studies, four used vital teeth as negative controls, which could also be regarded as biological/healthy controls .…”

Section: Discussionmentioning

confidence: 99%

“…Among these 12 studies, four used vital teeth as negative controls, which could also be regarded as biological/healthy controls . However, ten of 12 studies have assessed their negative controls solely by means of culture or PCR, whereas only two of 12 studies have actually sequenced their negative controls …”

Section: Discussionmentioning

confidence: 99%

“…Further studies that relied on FISH confirmed the presence of Synergistetes taxa in close proximity to apical lesions, more abundantly in SAPs, but were unable to assign lower taxonomy . Interestingly, clones BA121 and W090 respectively belong to the genera currently named Pyramidobacter and Fretibacterium , both of which have been abundantly detected in SAPs by several NGS reports included here (Table ) …”

Section: Discussionmentioning

confidence: 99%

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A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

Manoil

Al‐Manei

Belibasakis

2020

Proteomics Clinical Apps

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Next-generation sequencing (NGS) has now been applied for a decade to characterize the microbiota composition of infected dental root canals associated with apical periodontitis. Here, the study aims at systematically and critically reviewing these reports within the outcome of interest selected; the microbiota composition in different endodontic infection types. Standard methodological guidelines as stated by the PRISMA and the Joanna Briggs Institute are followed, including a risk of bias assessment. A literature search is conducted using the PubMed Advanced-Search Builder on April 8, 2019; only original research articles that investigated the microbiota of infected root-canals by means of NGS are screened. Among the 26 articles initially identified, 18 are included and evaluated for the following parameters; sampling protocol, sequencing strategy, and microbiota composition. The endodontic infections include primary apical periodontitis (PAP), secondary apical periodontitis (SAP), and apical abscess (AA). All infection types are associated with a highly diverse microbiota. Although some taxa appear differentially abundant between PAPs, SAPs, and AAs, no evident clustering of the microbiota by infection type is observed. These studies collectively formulate a comprehensive map of the taxa associated with endodontic infections and provide evidence of compositionally unspecific, yet abundance differentiates, community profiles according to clinical diagnosis.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 89%