Microbial and Enzymatic Biotransformations of Asphaltenes

Hernández-López, Edna L.; Ayala, Marcela; Vázquez-Duhalt, Rafael

doi:10.1080/10916466.2015.1014960

Cited by 31 publications

(14 citation statements)

References 65 publications

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“…Degradation of asphaltene is very difficult due to its complex structure. However, several microorganisms have been reported to degrade asphaltene including: 15.5% asphaltene biodegradation by Neosatorya fischeri after 11 weeks ; 15–40% biodegradation by some tropical fungal strains of Indonesia incubated for 30 days ; 48% by the mixed culture of five bacteria incubated for 60 days ; 77% by Pestalotiopsis sp. NG007 following incubation for 30 days .…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

et al. 2018

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Petroleum, as the major energy source, is indispensable from our lives. Presence of compounds resistant to degradation can pose risks for human health and environment. Basidiomycetes have been considered as powerful candidates in biodegradation of petroleum compounds via secreting ligninolytic enzymes. In this study a wood-decaying fungus was isolated by significant degradation ability that was identified as Daedaleopsis sp. by morphological and molecular identification methods. According to GC/MS studies, incubation of heavy crude oil with Daedaleopsis sp. resulted in increased amounts of C24 compounds. Degradation of asphaltene, anthracene, and dibenzofuran by the identified fungal strain was determined to evaluate its potential in biodegradation. After 14 days of incubation, Daedaleopsis sp. could degrade 93.7% and 91.2% of anthracene and dibenzofuran, respectively, in pH 5 and 40 °C in optimized medium, as revealed by GC/FID. Notably, analysis of saturates, aromatics, resins, and asphaltenes showed a reduction of 88.7% and 38% in asphaletene and aromatic fractions. Laccase, lignin peroxidase, and manganese peroxidase activities were enhanced from 51.3, 145.2, 214.5 U ml in the absence to 121.5, 231.4, and 352.5 U ml in the presence of heavy crude oil, respectively. This is the first report that Daedaleopsis sp. can degrade asphaltene and dibenzofuran. Moreover, compared to the reported results of asphaltene biodegradation, this strain was the most successful. Thus, Daedaleopsis sp. could be a promising candidate for biotransformation of heavy crude oil and biodegradation of recalcitrant toxic compounds.

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Section: Discussionmentioning

confidence: 99%

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

et al. 2018

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“…Resinous-asphaltene substances are hydrophobic, they envelope the plant roots blocking the water income, which results in plant drying. However, due to complicated biocenosis system (primarily to the content of hydrolyzing and oxidizing enzymes including polyphenol oxidases and peroxidases), the soils themselves are able to utilize a part of oil components by biochemical degradation and oxidative destruction to the point of conver- sion of certain part of them into organic matters consumed by plants [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Microbiological Oxidation of High Viscosity Bitumen in Soil

Filatov

Kopytov

Ovsyannikova

et al. 2018

Eurasian Chem. Tech. J.

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This paper presents the results of an investigation of microbiological oxidation in the model soil system of high viscosity bitumen from the Bayan-Erkhet deposit (Mongolia) with a high content of heteroelements. It is shown that bitumen, being a mixture of high molecular weight components, has no inhibitory effect on the indigenous soil microflora. Its active growth in the presence of oil products starts without adaptation and lasts for a good part of experiment resulting in 15-30 fold excess of microorganisms over its reference number. The enzymatic activity of the contaminated soil increases by a factor of 1.5-2.0, which indicates an assimilation of various hydrocarbon compounds. The weight analysis revealed that the biodegradation of oil products after 180 days of the experiment was 50% of the initial contamination at initial waste oil concentration 50 g/kg (5%). The analysis by IR spectroscopy revealed an accumulation of oxygen-containing compounds which are intermediate products of bio-oxidation of bitumen components. The method of chromatography-mass spectrometry (GC-MS) revealed the ability of aboriginal soil microflora to mineralize virtually all hydrocarbons contained in the bitumen under study. Their biodegradation ranges from 18 to 97%. It was shown by the GC-MS method that high-molecular heteroatomic components of bitumen (resins and asphaltenes) also undergo a microbial degradation, since their molecular structure changed after the destruction. Thus, the number of structural units in a hypothetical molecule and that of heteroatoms increased due to the high content of oxygen-containing structures. In addition, the ratio of hydrocarbons (oils), resins, and asphaltenes contained in the sample is also changed.

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“…Slow kinetics and an effective biodegradative molecular size limit of C44 has been observed in anaerobic reservoir conditions containing indigenous microbial communities 13,54,55 which have adapted to nutrient-deprived, growth arrested phenotypes over diagenesis 56 . Studies which have succeeded in biodegrading asphaltenes with microbial cultures have always done so under aerobic conditions 57–59 . Prior studies have offered hints that hydrolytic enzymes could potentially be used biodegrade petroleum compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Another researcher in China has reported the biodegradation of crude oil by a fungal enzyme mixture prepared from a pseudomonas species which reportedly contained lipase 64 . There are even a number of research groups currently biodegrading asphaltenes with a range of other enzymes which have been isolated and enriched from indigenous heavy oil degrading communities 59 including some which were chosen specifically for their lignocellulytic ability 65 .…”

Section: Resultsmentioning

confidence: 99%

Release of sugars and fatty acids from heavy oil biodegradation by common hydrolytic enzymes

Mislan¹,

Gates²

2019

Sci Rep

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In response to recent advances in understanding relating to the remarkable persistence of soil organic matter during burial and diagenesis, we examine the extent to which bitumen compositionally reflects the soil organic matter from which it was derived. Through a simple set of experiments, exposure of bitumen to lipase and cellulase, two enzymes effective in the biodegradation of soil organic matter, resulted in the release of glycerin, palmitic and oleic fatty acids from lipase digestion in addition to the release of glucose, alkylphenols and acyclic polyols from fermentation with cellulase, consistent with the products expected these enzymes. These results are significant in that they suggest that heavy oils are more similar to their soil precursor than previously thought, that biodegradation of bitumen can be accelerated using common over the counter enzymes in aerobic conditions and that heavy oils, which are 1000 times more abundant than coal, can release similar biomolecules as those generated in bioreactor culture or biomass harvest, using two of the most abundantly produced enzymes presently available.

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Microbial and Enzymatic Biotransformations of Asphaltenes

Cited by 31 publications

References 65 publications

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Microbiological Oxidation of High Viscosity Bitumen in Soil

Release of sugars and fatty acids from heavy oil biodegradation by common hydrolytic enzymes

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